Isolation and partial characterization of serum immunoglobuline in two species of sturgeons, Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser stellatus)

Yıl 2018, Cilt: 7 Sayı: 1, 1 - 3, 30.06.2018

Azadeh Yektaseresht Zahra Hemati Amin Gholamhosseini

https://doi.org/10.33714/masteb.375727

Öz

The immunoglobulins (Igs) were isolated and purified respectively from serum
of Siberian sturgeon (Acipenser baerii) and Stellate sturgeon (Acipenser
stellatus) by using 50% saturated ammonium sulfate precipitation. Analysis of the
purified Ig of the two species of fishes on sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE) under reducing conditions revealed two bands,
heavy chains and light chains respectively. The molecular weight of the heavy
chains of two fishes was about 70 kD and the molecular weight of the light chains
was 27kD for Siberian sturgeon and 27.5 kD for Stellate sturgeon. The results
suggested that the isolated and purified serum Immunoglobuline in two species of
sturgeons, Siberian sturgeon (A. baerii) and Stellate sturgeon (A. stellatus) can
be used to produce polyclonal and monoclonal antibodies against sturgeon Ig for
developing diagnostic tests against a wide variety of pathogens.

Anahtar Kelimeler

Immunoglobulins, serum, Acipenser baerii, Acipenser stellatus

Kaynakça

• Adkison, M.A., Basurco, B. & Hedrick, R.P. (1996). Humoral immunoglobulins of the white sturgeon, Acipenser transmontanus: partial characterization of and recognition with monoclonal antibodies. Developmental and Comparative Immunology, 20(4), 285-298.
• Al-Harbi, A. H., Truax, R. & Thune, R. L. (2000). Production and characterization of monoclonal antibodies against tilapia Oreochromisniloticus immunoglobulin. Aquaculture, 188, 219-227.
• Bag, M.R., Makesh, M., Rajendran, K.V. & Mukherjee, S.C. (2009). Characterization of IgM of Indian major carps and their cross-reactivity with anti-fish IgM antibodies. Fish & Shellfish Immunology, 26, 275-278.
• Bourmaud, C. A., Romestand, B. & Bouix, G. (1995). Isolation and partial characterization of IgM-like seabass (Dicentrarchuslabrax L. 1758) immunoglobulins. Aquaculture, 132, 53-58.
• Buchmann, K., Ostergaard, L. & Glamann, J. (1992). Affinity purification of antigen-specific serum immunoglobulin from the European eel (Anguilla anguilla). Scandinavian Journal of Immunology, 36, 89-98.
• Choudhury, M. & Pani Prasad, K. (2011). Isolation and characterization of immunoglobulin M of Asian sea bass, Latescalcarifer and its level in serum. Central European journal of biology, 6(2), 180-187.
• Crosbie, P. B. B. & Nowak, B. F. (2002). Production of polyclonal antisera against barramundi (Latescalcarifer Bloch) serum immunoglobulin derived from affinity columns containing mannan-binding protein or staphylococcal protein A. Aquaculture, 211, 49-63.
• Ellis, A. E. 1982. Differences between the immune mechanisms of fish and higher vertebrates. In Microbial diseases of fish, Eds., Roberts, R. J. Academic Press, London. 1-29 pp:
• Havarstein, L.S., Aasjord, P.M., Ness, S. & Endresen, C. (1988). Purification and partial characterization of an IgM like serum immunoglobulin from atlantic salmon (Salmosalar). Developmental and Comparative Immunology, 12,773-785.
• Hedrick, R.P., McDowell, T.S., Ahne, W., Torhy, C. & Kinkelin, P. (1992). Proerties of three iridovirus-like agents associated with systemic infections of fish. Diseases of Aquatic Organisms, 13, 203-209.
• Kofod, H., Pedersen, K., Larsen, J.L. & Buchmann, K. (1994). Purification and characterization of IgM- like immunoglobulin from turbot (Scophthalmusmaximus L.). Acta Veterinaria Scandinavica, 35, 1-10.
• Koumansvandiepen, J. C. E., Egberts, E., Peixoto, B. R., Taverne, & N. Rombout, J. H. W. M. (1995). B-cell and immunoglobulin heterogeneity in carp (Cyprinus carpio L.) an immuno(cyto)chemical study. Developmental and Comparative Immunology, 19, 97–108.
• Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature, 227, 680-685.
• LaPatra, S.E., Groff, J.M., Jones, G.R., Munn, B., Patterson, T.L., Holt, R.A., Hauck, A.K. & Hedrick, R.P. (1994). Occurrence of white sturgeon iridovirus infections among cultured white sturgeon in the Pacific Northwest. Aquaculture, 126, 201-210.
• Magnadottir, B., Kristjansdottir, H. & Gudmundsdottir, S. (1996). Characterization of monoclonal antibodies to separate epitopes on salmon IgM heavy chain. Fish & Shellfish Immunology, 6, 185-198.
• Palenzuela, O., Sitja-Bobadilla, A. & Alvarez-Pellitero, P. (1996.) Isolation and partial characterization of serumimmunoglobulins from sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparusaurata L.). Fish & Shellfish Immunology, 6, 81-94.
• Pilstrom, L. & Petersson, A. (1991). Isolation and partial characterization of immunoglobulin from cod (Gadusmorhua L.). Developmental and Comparative Immunology, 15, 143-152.
• Rathore, G., Swaminathan, T. R., Sood, N., Mishra, B. N. & Kapoor, D. (2006). Affinity purification and partial characterization of serum immunoglobulin of Clariasgariepinus. Indian Journal of Experimental Biology, 44, 1018-1021.
• Rathore, G., Kumar, G., Sood, N., Kapoor, D. & Lakra, W.S. (2008). Development of monoclonal antibodies to rohu (Labeorohita) immunoglobulins for use in immunoassays. Fish & Shellfish Immunology, 25, 761-774.
• Soltani, M. & Kalbassi, M.R. (2001). Protection of Persian sturgeon (Acipenser persicus) fingerling against Aeromonas hydrophila septicemia using three different antigens. Bulletin of the European Association of Fish Pathologists, 21, 235-239.
• Swain, T., Mohanty, J. & Sahu, A.K. (2004). One step purification and partial characterization of serum immunoglobulin from Asiatic catfish (Clariasbatrachus L.). Fish & Shellfish Immunology, 17, 397-401.
• Uchida, D., Hirose, H., Chang, P.K., Aranishi, F., Hirayabu, E., Mano, N., Mitsuya, T., Prayitno, S.B. & Natori, M., (2000). Characterization of Japanese eel immunoglobulin M and its level inserum. Comparative Biochemistry and Physiology, 127, 525-532.
• Vesely, T., Reschova, S., Pokorova, D., Hulova, J. & Nevorankova, Z. (2006). Production of monoclonal antibodies against immunoglobulin heavy chain in common carp (Cyprinus carpio L.). Veterinarni medicina, 51, 296–302.
• Watson, L.R., Yun, S.C., Groff, J.M. & Hedrick, R.P. (1995). Characteristics and pathogenicity of a novel herpesvirus isolated from adult and subadult white sturgeon Acipenser transmontaneus. Diseases of Aquatic Organisms, 22, 199-210.
• Watson, L.R., Groff, J.M. & Hedrick, R.P. (1998). Replication and pathogenesis of white sturgeon iridovirus in experimentally infected white sturgeon Acipenser transmontaneus juveniles and sturgeon cell lines. Diseases of Aquatic Organisms, 32, 173-184.
• Watts, M., Munday, B. L. & Burke, C. M. (2001). Isolation andpartial characterisation of immunoglobulin from southern bluefin tuna ThunnusmaccoyiiCastelnau. Fish & Shellfish Immunology,11,491-503.