Genomic and Transcriptomic Sequencing and Analysis Approaches

Yıl 2018, Cilt: 4 Sayı: 1, 34 - 42, 20.04.2018

Ümmügülsüm Tanman Zıplar Demet Cansaran-duman Mine Türktaş

https://doi.org/10.19127/mbsjohs.370490

Öz

In
this review, we explained that genomic and transcriptomic sequences and
analysis assays. First of all, we detailed information of genomic and
transcriptomic terms and related analyses. Genomics is aimed to elucidate the
structural and functional properties of the genomes. Transcriptomics are used
to express quantities of transcripts in a physiological state and specific
developmental stage. The methods of genomic and transcriptomic analyses imply
highly productive sequence analysis or microarray hybridization analysis as
well as bioinformatics analyses. The sequencing technologies include set of methods
such as preparing template, sequencing, imaging and data analysis. Firstly, the
nucleotide information peculiar to DNA and RNA is obtained by means of the
chosen technology in accordance with the goal and scope of the study. The
obtained sequences are aligned with respect to a familiar reference sequence,
or are combined as de novo. Subsequently,
it is determined whether the distinct genomic sets are connected with the other
genomic sets by overlapping distinct genomic sets, such as aligned sequence
readings, gene annotation, EST, genetic
polymorphism, and mobile elements. So, the structural variant to which the
obtained sequence data are peculiar is determined. Within the scope of the study,
giving information about sequencing technologies and the methods of analyses of
the obtained sequences is aimed for researchers work on this subject.

Anahtar Kelimeler

Genomics, Transcriptomics, Bioinformatics

Kaynakça

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