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Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey

Yıl 2017, Cilt: 30 Sayı: 1, 27 - 33, 04.04.2017

Öz

Chickpea (Cicer arietinum L.) is an important food legume crop and Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is one of the most important diseases of chickpea in Turkey. Fusarium redolens is known to cause wilt-like disease of chickpea in other countries, but has not been reported from Turkey. Accurate identification of pathogen species and races is important for managing the disease in developing and deploying resistant cultivars. Forty five Fusarium spp. isolates caused wilt of chickpea were obtained from 10 provinces in Turkey from 2007 to 2009. Species level identification of these isolates was done using PCR primers specific for F. oxysporum f. sp. ciceris and F. redolens along with sequencing of the Translation Elongation Factor 1-α (Ef-1α) gene region. Among the isolate collection, eight isolates were identified as F. redolens, and eleven isolates as F. oxysporum f. sp. ciceris, based on PCR with species-specific primers. Seven of the eleven F. oxysporum f. sp. ciceris isolates were further identified as race 0, based on PCR with race-specific primers. Moreover, sequence data of Ef-1α region were used to identify any isolate that did not give amplification with F. oxysporum f. sp. ciceris race specific primers and F. redolens specific primers. This is the first report of F. redolens causing chickpea wilt in Turkey.




Kaynakça

  • Al-Taae AK, Hadwan HA, Al-Jobory SAE (2013) Physiological races of Fusarium oxysporum f. sp. ciceris in Iraq. Journal of Life Sciences 7: 1070-1075.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403-410.
  • Armstrong GM, Armstrong JK (1981) Formae speciales races of Fusarium oxysporum causing wilt diseases. In: Nelson PE, Toussoun TA, Cook RJ, editors. Fusarium: Diseases, Biology and Taxonomy, The Pennsylvania State University Press, University Park, p. 391-399.
  • Asan A. (2011) Checklist of Fusarium species reported from Turkey. Mycotaxon 116: 479.
  • Baayen RP, Van Dreven F, Krijger MC, Waalwijk C (1997) Genetic diversity in Fusarium oxysporum f. sp. dianthi and Fusarium redolens f. sp. dianthi. European Journal of Plant Pathol 103: 395–408.
  • Baayen RP, O’Donnell K, Bonants PJM. Cigelnik E, Kroon LPN, Roebroeck EJA, Waalwijk C (2000) Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and non-monophyletic formae speciales causing wilt and rot diseases. Phytopathol 90: 891-900.
  • Baayen RP, O’Donnell K, Breeuwsma S, Geiser DM, Waalwijk C (2001) Molecular relationships of fungi within the Fusarium redolens–F. hostae clade. Phytopathol, 91: 1037-1044.
  • Bayraktar H, Dolar FS, Maden S (2008) Use of RAPD and ISSR Markers in Detection of Genetic Variation and Population Structure among Fusarium oxysporum f. sp. ciceris Isolates on Chickpea in Turkey. J Phytopathol, 156: 146–154.
  • Bayraktar H, Dolar FS (2009) Genetic diversity of wilt and root rot pathogens of chickpea, as assessed by RAPD and ISSR. Turk. J. Agric. For., 33: 1-10.
  • Bayraktar H, Dolar FS (2012) Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pak. J. Bot., 44: 821-823.
  • Bienapfl JC, Malvick DK, Percich JA (2010) First report of Fusarium redolens causing root rot of soybean in Minnesota. Plant Dis, 94: 1069.
  • Bogale M, Wingfield BD, Wingfield MJ, Steenkamp ET (2006) Characterization of Fusarium oxysporum isolates from Ethiopia using AFLP, SSR, and DNA sequence analyses. Fungal Divers, 23: 51-66.
  • Bogale M, Wingfield BD, Wingfield MJ, Steenkamp MJ (2007) Species-specific primers for Fusarium redolens and a PCR-RFLP technique to distinquish among three clades of Fusarium oxysporum. FEMS Microbiol Lett 271: 27-32.
  • Booth C (1971) The Genus Fusarium. Commonwealth Mycological Institute, Kew.
  • Booth C (1977) Fusarium Laboratory Guide to the Identification of the Major Species. CMI, UK.
  • Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D. (1994) Laboratory Manual of Fusarium Research. Departmen of Crop Sciences, University of Sydney, Sydney, Australia.
  • Cabrera J, Colina DL, Trapero-Casas A, Jimenz-Diaz RM (1989) Races of Foc in Andalusia, South Spain. International Chickpea Newsletter 13: 24-26.
  • Chen W (2011) Fusarium wilt of chickpea. In: Chen W, Sharma HC, Muehlbauer FJ (eds.) Compendium of Chickpea and Lentil Diseases and Pests. APS Press, St. Paul, p. 16-20.
  • Clarkson JDS (1978) Pathogenicity of Fusarium spp. associated with foot-rots of peas and beans. Plant Pathol 27: 110-117.
  • Dolar FS (1997) Determination of the races of Fusarium oxysporum f. sp. ciceris in Ankara province of Turkey. Journal of Turkish Phytopathogy 26: 11-15.
  • Dubey SC, Priyanka K, Upadhyay BK (2014) Development of molecular markers and probes based on TEF-1α, β-tubulin and ITS gene sequences for quantitative detection of Fusarium oxysporum f. sp. ciceris by using real-time PCR. Phytoparasitica 42: 355-366.
  • Edel V, Steinberg C, Avelange I, Laguerre G, Alabouvette C (1995) Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology 85: 579-585.
  • Edel-Hermann V, Gautheron N, Steinberg C (2012) Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathol 61: 787-800.
  • Gambhir A, Lamppa RS, Rasmussen JB, Goswami RS (2010) Evaluation of aggressiveness and host range of Fusarium acuminatum and Fusarium redolens associated with root rot of dry beans. Phytopathology 100: 185.
  • Geiser DM, Jiménez-Gasco MM, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K (2004) FUSARIUM-Iv. 1.0: a DNA sequence database for identifying Fusarium. Eur J Plant Pathol, 110: 473-479.
  • Gurjar G, Barve M, Giri A, Gupta V (2009) Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia, 101: 484-495.
  • Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis. Nucleic Acids Symposium Series, 41: 95-8.
  • Halila HM, Strange RN (1996) Identification of the causal agent of wilt of chickpea in Tunisia as Fusarium oxysporum f. sp. ciceris race 0. Phytopathologia Mediterranea, 35: 67-74.
  • Haware MP, Nene YL (1982) Races of Fusarium oxysporum f. sp. ciceri. Plant Dis, 66: 809-810.
  • Jiménez-Díaz RM, Alcalá-Jiménez AR, Hervás A, Trapero-Casas JL. (1993) Pathogenic variability and hosts resistance in the Fusarium oxysporum f. sp. ciceris / Cicer arietinum pathosystem. In: Proc. 3rd Eur. Semin. Fusarium Mycotoxins, Taxonomy, Pathogenicity and Host Resistance, Hodowsla Ròslin Aklimatyazacja i Nasiennictwo. Plant Breeding and Acclimatization Institute, Radzikóv, Poland, p. 87-94.
  • Jiménez-Gasco MM, Perez-Artes E, Jimenez-Diaz RM (2001) Identification of pathogenic races 0 1B/C, 5 and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). Eur J Plant Pathol 51: 72-77.
  • Jiménez-Gasco MM, Jiménez-Díaz RM (2003) Development of a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5, and 6. Phytopathology, 93: 200-209.
  • Jimenez-Fernandez D, Navas-Cortes JA, Montes-Borrego M, Jiménez-Díaz RM, Landa BB (2011) Molecular and pathogenic characterization of Fusarium redolens, a new causal agent of Fusarium yellows in chickpea. Plant Dis 95: 860-870.
  • Kraft JM, Haware MP, Hussein MM (1988) Root rot and wilt diseases of food legumes. In: Summerfield RJ (ed) World Crops: Cool Season Food Legumes, Kluwer Academic Publishers, Dordrecht, p. 565-575.
  • Leisso R, Miller Z, Jacobsen B, Burrows M (2011) Pathogenicity of Fusarium spp. to chickpea seed and seedling (Cicer arietinum L.). Can J Plant Pathol 33: 400-409.
  • Leslie JF, Zeller KA, Summerell BA (2001) Icebergs and species in populations of Fusarium. Physiol. Mol. Plant Pathol., 59: 107-117.
  • Michelse CB, Rep M (2009) Pathogen profile update: Fusarium oxysporum. Mol Plant Pathol 10: 311–324.
  • Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park.
  • O’Donnell K, Kistler HC, Cigelink E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. PNAS USA, 95: 2044-2049.
  • O'Donnell K, Gueidan C, Sink S, Johnston PR, Crous PW, Glenn A, et al. (2009) A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genet Biol 46: 936-938.
  • Phillips JC (1988) A distinct race of chickpea wilt in California. International Chickpea Newsletters 18: 19–20.
  • Rahman ML, Haware MP, Mian IH, Akanda AM (1998) Races of Fusarium oxysporum f. sp. ciceris causing chickpea wilt in India. Bangladesh Journal of Plant Pathology, 14: 33–36
  • Riccioni L, Haegi A, Valvassori M (2008) First report of vascular wilt caused by Fusarium redolens on lentil in Italy. Plant Dis 92: 1132.
  • Sharma KD, Chen W, Muehlbauer FJ (2005) Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Dis 89: 385-390.
  • Sharma KD, Muehlbauer FJ (2007) Fusarium wilt of chickpea: physiological specialization, genetics of resistance and resistance gene tagging. Euphytica, 157: 1-14.
  • Tekeoglu M, Tullu A, Kaiser WJ, Muehlbauer FJ (2000) Inheritance and linkage of two genes that confer resistance to Fusarium wilt in chickpea. Crop Sci, 40: 1247-1251.
  • Visser C, Wingfield MJ, Wingfield BD, Yamaoka Y (1995) Ophiostoma polonicum is a species of Ceratosystis sensu stricto. Syst App Microbiol, 18: 403-409.
  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.) PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., New York, pp. 315-322.

Türkiye’de nohutta solgunluğa neden olan Fusarium spp.’nin moleküler tanımlaması ve Fusarium redolens’in ilk raporu

Yıl 2017, Cilt: 30 Sayı: 1, 27 - 33, 04.04.2017

Öz



Nohut
(Cicer arietinum L.) önemli bir
yemeklik baklagil türüdür ve Fusarium
oxysporum
f. sp. ciceris
tarafından neden olunan Fusarium solgunluğu Türkiye’de en önemli nohut
hastalıklarından biridir. Fusarium
redolens
’in bazı ülkelerde nohutta solgunluk benzeri hastalığa neden olduğu
bilinmektedir, ancak Türkiye’den rapor edilmemiştir. Patojen tür ve ırklarının
doğru tanımlanması, hastalık yönetimi için dayanıklı çeşit geliştirme ve
kullanımında önemlidir. Nohutta solgunluğa neden olan kırkbeş Fusarium spp. izolatı 2007’den 2009’a
kadar Türkiye’deki 10 ilden elde edilmiştir. Bu izolatların tür seviyesinde
tanımlamaları F. oxysporum f. sp. ciceris ve F. redolens için spesifik PCR primerleri ve Translation Elongation Factor 1-α (Ef-1α) gen bölgesinin sekanslanması ile yapılmıştır. İzolat
koleksiyonunda, tür spesifik primerlerle yapılan PCR’a göre, sekiz izolat F. redolens ve onbir izolat F. oxysporum f. sp. ciceris olarak tanımlanmıştır. Onbir F. oxysporum f. sp. ciceris
izolatının yedisi ırk-spesifik primerlerle yapılan PCR’a göre ırk 0 olarak
tanımlanmıştır. Ayrıca, F. oxysporum
f. sp. ciceris ırk-spesifik ve F. redolens tür-spesifik primerlerle
ürün vermeyen izolatların tanımlamalarında Ef-1α
bölgesinin sekans verisi kullanılmıştır. Bu, Türkiye’de nohutta solgunluğa
neden olan F. redolens’in ilk
raporudur.




Kaynakça

  • Al-Taae AK, Hadwan HA, Al-Jobory SAE (2013) Physiological races of Fusarium oxysporum f. sp. ciceris in Iraq. Journal of Life Sciences 7: 1070-1075.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. Journal of Molecular Biology 215: 403-410.
  • Armstrong GM, Armstrong JK (1981) Formae speciales races of Fusarium oxysporum causing wilt diseases. In: Nelson PE, Toussoun TA, Cook RJ, editors. Fusarium: Diseases, Biology and Taxonomy, The Pennsylvania State University Press, University Park, p. 391-399.
  • Asan A. (2011) Checklist of Fusarium species reported from Turkey. Mycotaxon 116: 479.
  • Baayen RP, Van Dreven F, Krijger MC, Waalwijk C (1997) Genetic diversity in Fusarium oxysporum f. sp. dianthi and Fusarium redolens f. sp. dianthi. European Journal of Plant Pathol 103: 395–408.
  • Baayen RP, O’Donnell K, Bonants PJM. Cigelnik E, Kroon LPN, Roebroeck EJA, Waalwijk C (2000) Gene genealogies and AFLP analyses in the Fusarium oxysporum complex identify monophyletic and non-monophyletic formae speciales causing wilt and rot diseases. Phytopathol 90: 891-900.
  • Baayen RP, O’Donnell K, Breeuwsma S, Geiser DM, Waalwijk C (2001) Molecular relationships of fungi within the Fusarium redolens–F. hostae clade. Phytopathol, 91: 1037-1044.
  • Bayraktar H, Dolar FS, Maden S (2008) Use of RAPD and ISSR Markers in Detection of Genetic Variation and Population Structure among Fusarium oxysporum f. sp. ciceris Isolates on Chickpea in Turkey. J Phytopathol, 156: 146–154.
  • Bayraktar H, Dolar FS (2009) Genetic diversity of wilt and root rot pathogens of chickpea, as assessed by RAPD and ISSR. Turk. J. Agric. For., 33: 1-10.
  • Bayraktar H, Dolar FS (2012) Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pak. J. Bot., 44: 821-823.
  • Bienapfl JC, Malvick DK, Percich JA (2010) First report of Fusarium redolens causing root rot of soybean in Minnesota. Plant Dis, 94: 1069.
  • Bogale M, Wingfield BD, Wingfield MJ, Steenkamp ET (2006) Characterization of Fusarium oxysporum isolates from Ethiopia using AFLP, SSR, and DNA sequence analyses. Fungal Divers, 23: 51-66.
  • Bogale M, Wingfield BD, Wingfield MJ, Steenkamp MJ (2007) Species-specific primers for Fusarium redolens and a PCR-RFLP technique to distinquish among three clades of Fusarium oxysporum. FEMS Microbiol Lett 271: 27-32.
  • Booth C (1971) The Genus Fusarium. Commonwealth Mycological Institute, Kew.
  • Booth C (1977) Fusarium Laboratory Guide to the Identification of the Major Species. CMI, UK.
  • Burgess LW, Summerell BA, Bullock S, Gott KP, Backhouse D. (1994) Laboratory Manual of Fusarium Research. Departmen of Crop Sciences, University of Sydney, Sydney, Australia.
  • Cabrera J, Colina DL, Trapero-Casas A, Jimenz-Diaz RM (1989) Races of Foc in Andalusia, South Spain. International Chickpea Newsletter 13: 24-26.
  • Chen W (2011) Fusarium wilt of chickpea. In: Chen W, Sharma HC, Muehlbauer FJ (eds.) Compendium of Chickpea and Lentil Diseases and Pests. APS Press, St. Paul, p. 16-20.
  • Clarkson JDS (1978) Pathogenicity of Fusarium spp. associated with foot-rots of peas and beans. Plant Pathol 27: 110-117.
  • Dolar FS (1997) Determination of the races of Fusarium oxysporum f. sp. ciceris in Ankara province of Turkey. Journal of Turkish Phytopathogy 26: 11-15.
  • Dubey SC, Priyanka K, Upadhyay BK (2014) Development of molecular markers and probes based on TEF-1α, β-tubulin and ITS gene sequences for quantitative detection of Fusarium oxysporum f. sp. ciceris by using real-time PCR. Phytoparasitica 42: 355-366.
  • Edel V, Steinberg C, Avelange I, Laguerre G, Alabouvette C (1995) Comparison of three molecular methods for the characterization of Fusarium oxysporum strains. Phytopathology 85: 579-585.
  • Edel-Hermann V, Gautheron N, Steinberg C (2012) Genetic diversity of Fusarium oxysporum and related species pathogenic on tomato in Algeria and other Mediterranean countries. Plant Pathol 61: 787-800.
  • Gambhir A, Lamppa RS, Rasmussen JB, Goswami RS (2010) Evaluation of aggressiveness and host range of Fusarium acuminatum and Fusarium redolens associated with root rot of dry beans. Phytopathology 100: 185.
  • Geiser DM, Jiménez-Gasco MM, Kang S, Makalowska I, Veeraraghavan N, Ward TJ, Zhang N, Kuldau GA, O’Donnell K (2004) FUSARIUM-Iv. 1.0: a DNA sequence database for identifying Fusarium. Eur J Plant Pathol, 110: 473-479.
  • Gurjar G, Barve M, Giri A, Gupta V (2009) Identification of Indian pathogenic races of Fusarium oxysporum f. sp. ciceris with gene specific, ITS and random markers. Mycologia, 101: 484-495.
  • Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis. Nucleic Acids Symposium Series, 41: 95-8.
  • Halila HM, Strange RN (1996) Identification of the causal agent of wilt of chickpea in Tunisia as Fusarium oxysporum f. sp. ciceris race 0. Phytopathologia Mediterranea, 35: 67-74.
  • Haware MP, Nene YL (1982) Races of Fusarium oxysporum f. sp. ciceri. Plant Dis, 66: 809-810.
  • Jiménez-Díaz RM, Alcalá-Jiménez AR, Hervás A, Trapero-Casas JL. (1993) Pathogenic variability and hosts resistance in the Fusarium oxysporum f. sp. ciceris / Cicer arietinum pathosystem. In: Proc. 3rd Eur. Semin. Fusarium Mycotoxins, Taxonomy, Pathogenicity and Host Resistance, Hodowsla Ròslin Aklimatyazacja i Nasiennictwo. Plant Breeding and Acclimatization Institute, Radzikóv, Poland, p. 87-94.
  • Jiménez-Gasco MM, Perez-Artes E, Jimenez-Diaz RM (2001) Identification of pathogenic races 0 1B/C, 5 and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). Eur J Plant Pathol 51: 72-77.
  • Jiménez-Gasco MM, Jiménez-Díaz RM (2003) Development of a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5, and 6. Phytopathology, 93: 200-209.
  • Jimenez-Fernandez D, Navas-Cortes JA, Montes-Borrego M, Jiménez-Díaz RM, Landa BB (2011) Molecular and pathogenic characterization of Fusarium redolens, a new causal agent of Fusarium yellows in chickpea. Plant Dis 95: 860-870.
  • Kraft JM, Haware MP, Hussein MM (1988) Root rot and wilt diseases of food legumes. In: Summerfield RJ (ed) World Crops: Cool Season Food Legumes, Kluwer Academic Publishers, Dordrecht, p. 565-575.
  • Leisso R, Miller Z, Jacobsen B, Burrows M (2011) Pathogenicity of Fusarium spp. to chickpea seed and seedling (Cicer arietinum L.). Can J Plant Pathol 33: 400-409.
  • Leslie JF, Zeller KA, Summerell BA (2001) Icebergs and species in populations of Fusarium. Physiol. Mol. Plant Pathol., 59: 107-117.
  • Michelse CB, Rep M (2009) Pathogen profile update: Fusarium oxysporum. Mol Plant Pathol 10: 311–324.
  • Nelson PE, Toussoun TA, Marasas WFO (1983) Fusarium Species: An Illustrated Manual for Identification. Pennsylvania State University Press, University Park.
  • O’Donnell K, Kistler HC, Cigelink E, Ploetz RC (1998) Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. PNAS USA, 95: 2044-2049.
  • O'Donnell K, Gueidan C, Sink S, Johnston PR, Crous PW, Glenn A, et al. (2009) A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genet Biol 46: 936-938.
  • Phillips JC (1988) A distinct race of chickpea wilt in California. International Chickpea Newsletters 18: 19–20.
  • Rahman ML, Haware MP, Mian IH, Akanda AM (1998) Races of Fusarium oxysporum f. sp. ciceris causing chickpea wilt in India. Bangladesh Journal of Plant Pathology, 14: 33–36
  • Riccioni L, Haegi A, Valvassori M (2008) First report of vascular wilt caused by Fusarium redolens on lentil in Italy. Plant Dis 92: 1132.
  • Sharma KD, Chen W, Muehlbauer FJ (2005) Genetics of chickpea resistance to five races of Fusarium wilt and a concise set of race differentials for Fusarium oxysporum f. sp. ciceris. Plant Dis 89: 385-390.
  • Sharma KD, Muehlbauer FJ (2007) Fusarium wilt of chickpea: physiological specialization, genetics of resistance and resistance gene tagging. Euphytica, 157: 1-14.
  • Tekeoglu M, Tullu A, Kaiser WJ, Muehlbauer FJ (2000) Inheritance and linkage of two genes that confer resistance to Fusarium wilt in chickpea. Crop Sci, 40: 1247-1251.
  • Visser C, Wingfield MJ, Wingfield BD, Yamaoka Y (1995) Ophiostoma polonicum is a species of Ceratosystis sensu stricto. Syst App Microbiol, 18: 403-409.
  • White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds.) PCR Protocols: A Guide to Methods and Applications, Academic Press, Inc., New York, pp. 315-322.
Toplam 48 adet kaynakça vardır.

Ayrıntılar

Bölüm Makaleler
Yazarlar

Mücella Tekeoğlu Bu kişi benim

Hilal Özkılınç

Berna Tunalı Bu kişi benim

İsmail Küsmenoğlu Bu kişi benim

Weidong Chen Bu kişi benim

Yayımlanma Tarihi 4 Nisan 2017
Gönderilme Tarihi 11 Nisan 2017
Yayımlandığı Sayı Yıl 2017 Cilt: 30 Sayı: 1

Kaynak Göster

APA Tekeoğlu, M., Özkılınç, H., Tunalı, B., Küsmenoğlu, İ., vd. (2017). Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences, 30(1), 27-33.
AMA Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W. Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences. Nisan 2017;30(1):27-33.
Chicago Tekeoğlu, Mücella, Hilal Özkılınç, Berna Tunalı, İsmail Küsmenoğlu, ve Weidong Chen. “Molecular Identification of Fusarium Spp. Causing Wilt of Chickpea and the First Report of Fusarium Redolens in Turkey”. Mediterranean Agricultural Sciences 30, sy. 1 (Nisan 2017): 27-33.
EndNote Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W (01 Nisan 2017) Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences 30 1 27–33.
IEEE M. Tekeoğlu, H. Özkılınç, B. Tunalı, İ. Küsmenoğlu, ve W. Chen, “Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey”, Mediterranean Agricultural Sciences, c. 30, sy. 1, ss. 27–33, 2017.
ISNAD Tekeoğlu, Mücella vd. “Molecular Identification of Fusarium Spp. Causing Wilt of Chickpea and the First Report of Fusarium Redolens in Turkey”. Mediterranean Agricultural Sciences 30/1 (Nisan 2017), 27-33.
JAMA Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W. Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences. 2017;30:27–33.
MLA Tekeoğlu, Mücella vd. “Molecular Identification of Fusarium Spp. Causing Wilt of Chickpea and the First Report of Fusarium Redolens in Turkey”. Mediterranean Agricultural Sciences, c. 30, sy. 1, 2017, ss. 27-33.
Vancouver Tekeoğlu M, Özkılınç H, Tunalı B, Küsmenoğlu İ, Chen W. Molecular identification of Fusarium spp. causing wilt of chickpea and the first report of Fusarium redolens in Turkey. Mediterranean Agricultural Sciences. 2017;30(1):27-33.

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