Genetic barcoding and preliminary phylogenetic analysis of Serranidae species from Maltese coastal waters, with a perspective on their Mediterranean phylogeography

Yıl 2016, Cilt: 1 Sayı: 3, 66 - 77, 21.12.2016

Adriana Vella Noel Vella

https://doi.org/10.28978/nesciences.286369

Cited By: 1

Öz

Ten species of the Serranidae Family sampled from the Mediterranean, including two non-native species, were identified using molecular genetic tools. Two mitochondrial genes, cytochrome c oxidase subunit I (COI) and Cytochrome b (Cyt b) genes were studied covering a total of 980 bp of which 360 bp exhibited genetic differences. Within species the members of the genus Serranus exhibited the highest haplotypic diversity, while the genera of larger grouper taxa have shown low haplotypic and nucleotide diversity indices with these genetic markers. Each sequence was also checked against BOLD and GenBank databases to compare species categorization. COI data on S. cabrilla and S. scriba were used in a preliminary phylogeographic analyses for these two species. Results show significant differences between certain sampling locations, indicating localized populations within the Mediterranean.

Anahtar Kelimeler

Serranidae, DNA barcoding, phylogenetics, phylogeography COI, Cyt b

Kaynakça

• Abdul Malak, D., Livingstone, SR., Pollard, D., Polidoro, B.A., Cuttelod, A., Bariche, M., Bilecenglu, M., Carpenter, K.E., Collette, B.B., Francour, P., Goren, M., Hichem Kara, M., Massutí, E., Papaconstantinou, C., Tunesi, L. (2011). Overview of the Conservation Status of the Marine Fishes of the Mediterranean Sea. IUCN, Gland, Switzerland, and Malaga, Spain.
• Andrello, M., Mouillot, D., Beuvier, J., Albouy, C., Thuiller, W., Manel, S. (2013). Low Connectivity between Mediterranean Marine Protected Areas: A Biophysical Modeling Approach for the Dusky Grouper Epinephelus marginatus. PLoS ONE, 8(7): e68564.
• Bandelt, H., Forster, P., Röhl, A. (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology & Evolution, 16(1), 37–48.
• Bariche, M., Heemstra, P. (2012). First record of the blacktip grouper Epinephelus fasciatus (Teleostei: Serranidae) in the Mediterranean Sea. Marine Biodiversity Records, 5: e1.
• Ben Abdallah, A., Ben Soussi, J., Méjri, H., Canapé, C., Golani, D. (2007). First record of Cephalopholis taeniops (Valenciennes) in the Mediterranean Sea. Journal of Fish Biology, 71, 610–614.
• Ben-Tuvia, A., Lourie, A. (1969). A Red Sea grouper Epinephelus tauvina caught on the Mediterranean coast of Israel. Israel Journal of Zoology, 18, 245–247.
• Buchholz-Sørensen, M., Vella, A. (2016). Population Structure, Genetic Diversity, Effective Population Size, Demographic History and Regional Connectivity Patterns of the Endangered Dusky Grouper, Epinephelus marginatus (Teleostei:Serranidae), within Malta’s Fisheries Management Zone. PLoS ONE, 11(7): e0159864.
• Coll, M., Piroddi, C., Steenbeek, J., Kaschner, K., Ben Rais Lasram, F., Aguzzi, J., Ballesteros, E., Bianchi, C.N., Corbera, J., Dailianis, T., Danovaro, R., Estrada, M., Froglia, C., Galil, B.S., Gasol, J.M., Gertwagen, R., Gil, J., Guilhaumon, F., Kesner-Reyes, K., Kitsos, M.S., Koukouras, A., Lampadariou, N., Laxamana, E., Lopez-Fe de la Cuadra, C.M., Lotze, H.K., Martin, D., Mouillot, D., Oro, D., Raicevich, S., Rius-Barile, J., Saiz-Salinas, J.I., San Vicente, C., Somot, S., Templado, J., Turon, X., Vafidis, D., Villanueva, R., Voultsiadou, E. (2010). The biodiversity of the Mediterranean Sea: estimates, patterns and threats. PLoS ONE, 5(8): e11842.
• Cornish, A. & Harmelin-Vivien, M. (2004). Epinephelus marginatus. The IUCN Red List of Threatened Species 2004: e.T7859A12857009. Accessed 10 Nov 2016
• Cornish, A. & Harmelin-Vivien, M. (2011). Epinephelus marginatus. The IUCN Red List of Threatened Species 2011: e.T7859A12856576. Accessed 10 Nov 2016
• Dulčić, J., Dragičević, B. (2013). Paranthias furcifer (Perciformes: Serranidae), a new alien fish in the Mediterranean Sea. Journal of Fish Biology, 82, 332-337.
• EUR-Lex (2016). EUR-Lex: Access to European Union Law. Retrieved from http://eur-lex.europa.eu/en/index.htm - Accessed 10 Nov 2016
• Excoffier L, Lischer HE. (2010). Arlequin suite ver 3.5: A new series of programs to perform population genetics analyses under Linux and Windows. Molecular Ecology Resources, 10, 564-567.
• FAO (2016). Fisheries Global Information System (FAO-FIGIS) - Web site. Fisheries Global Information System (FIGIS). FI Institutional Websites. In: FAO Fisheries and Aquaculture Department [online]. Rome. Accessed 10 Nov 2016.
• Froese, R. & Pauly, D. FishBase. (2016). URL http://www.fishbase.org. Accessed 10 Aug 2016.
• Gökoglu, M. & Özvarol, Y. (2015). Epinephelus coioides (Actinopterygii: Perciformes: Serranidae) - a new Lessepsian migrant in the Mediterranean coast of Turkey. Acta Ichthyologica et Piscatoria, 45(3), 307–309.
• Golani, D., Askarov, G., Dashevsky, Y. (2015). First record of the Red Sea spotted grouper, Epinephelus geoffroyi (Klunzinger, 1870) (Serranidae) in the Mediterranean. BioInvasions Records, 4(2), 143–5.
• Guidetti, P., Giardina, F., Azzurro, E. (2010). A new record of Cephalopholis taeniops in the Mediterranean Sea, with considerations on the Sicily channel as a biogeographical crossroad of exotic fish. Marine Biodiversity Records, 3: e13.
• Heemstra, P.C. & Golani, D. (1993). Clarification of the Indo-Pacific groupers (Pisces: Serranidae) in the Mediterranean Sea. Israel Journal of Zoology, 39, 381–390.
• Heemstra, P.C. & Randall, J.E. (1993). Groupers of the world (Family Serranidae, Subfamily Epinephelinae). An annotated and illustrated catalogue of the grouper, rockcod, hind, coral grouper and lyretail species known to date. Rome: FAO Fisheries Synopsis No. 125 Vol. 16. Food and Agriculture Organization of the United Nations. p. 382.
• Kearse, M., Moir, R., Wilson, A., Stones-Havas, S., Cheung, M., Sturrock, S., Buxton, S., Cooper, A., Markowitz, S., Duran, C., Thierer, T., Ashton, B., Mentjies, P., Drummond, A. (2012). Geneious Basic: an integrated and extendable desktop software platform for the organization and analysis of sequence data. Bioinformatics, 28, 1647–1649.
• Kimura, M. (1980). A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16, 111–120.
• Kumar, S., Stecher, G., Tamura, K. (2016). MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), msw054.
• Leigh, J.W. & Bryant, D. (2015). Popart: full-feature software for haplotype network construction. Methods in Ecology & Evolution, 6(9): 1110–1116.
• Lelong, P. (2005). Capture d’un macabit, Epinephelus merra Bloch, 1793 (Poisson, Serranidae), en Méditerranée nord- occidentale. Marine Life, 15(1-2):63–66.
• Martin, A.P. & Palumbi, S.R. (1993). Protein evolution in different cellular environments: cytochrome b in sharks and mammals. Molecular Biology and Evolution, 10(4), 873–891.
• Occhipinti-Ambrogi, A. & Galil, B. (2010). Marine alien species as an aspect of global change. Advances in Oceanography & Limnology, 1(1),199–218.
• Parenti, P. & Bressi, N. (2001). First record of the orange-spotted grouper, Epinephelus coioides (Perciformes: Serranidae) in the northern Adriatic Sea. Cybium, 25(3), 281–284.
• Rothman, S.B., Stern, N., Goren, M. (2016). First record of the Indo-Pacific areolate grouper Epinephelus areolatus (Forsskål, 1775) (Perciformes: Epinephelidae) in the Mediterranean Sea. Zootaxa, 4067(4), 479–483.
• Vella, A. (2009). Sustainable Fisheries in the Mediterranean. Regional Activity Centre for Cleaner Production (CP/RAC) Mediterranean Action Plan. Barcelona, Spain. 142 pp.
• Vella, A., Agius Darmanin, S., Vella, N. (2015a). Morphological and genetic barcoding study confirming the first Stegastes variabilis (Castelnau, 1855) report in the Mediterranean Sea. Mediterranean Marine Science, 16(3), 609-12.
• Vella, A., Vella, N., Agius Darmanin, S. (2015b). First record of Lutjanus fulviflamma (Osteichthyes: Lutjanidae) in the Mediterranean Sea. Journal of the Black Sea/Mediterranean Environment, 21(3), 307-15.
• Vella, A., Vella, N., Agius Darmanin, S. (2016a). The first record of the African sergeant, Abudefduf hoefleri (Perciformes: Pomacentridae), in the Mediterranean Sea. Marine Biodiversity Records, 9(1),1-5.
• Vella, N., Vella, A., Agius Darmanin, S. (2016b). Morphological and genetic analyses of the first record of the Niger Hind, Cephalopholis nigri (Perciformes: Serranidae), in the Mediterranean Sea and of the African Hind, Cephalopholis taeniops, in Malta. Marine Biodiversity Records, 9, e99.
• Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., Hebert, P.D. (2005). DNA barcoding Australia’s fish species. Philosophical Transactions of the Royal Society B: Biological Sciences, 360(1462), 1847–1857.