Farklı bitkisel yağların Streptomyces clavuligerus’ta klavulanik asit üretimi üzerine etkisi

Yıl 2017, Cilt: 32 Sayı: 2, 223 - 228, 20.06.2017

Aslıhan Kurt Kızıldoğan

https://doi.org/10.7161/omuanajas.287668

Cited By: 1

Öz

Bu çalışmada, karbon kaynağı olarak
farklı bitkisel yağların Streptomyces
clavuligerus ATCC27064 suşunda klavulanik asit üretimi üzerine etkisinin
belirlenmesi amaçlanmıştır. Fermentasyon besiyerinde zeytinyağı, mısırözü yağı,
ayçiçeği yağı ve fındık yağı varlığında 168 saat süresince 28^oC ve
220 rpm’de inkübe edilen kültürlerin kontrole kıyasla daha iyi üreme profili
gösterdiği bulunmuştur. Agar plak difüzyon deney sonuçlarına göre, ayçiçeği ve
zeytinyağında S. clavuligerus
ATCC27064 tarafından üretilen klavulanik asit miktarı kontroldekinden daha
düşük tespit edilmiştir. En yüksek klavulanik asit üretimi (1526.4 mg L^-1)
inkübasyonun 120. saatinde (T₁₂₀) ve ile fındık yağının kullanılması
ile elde edilmiştir. Sonuçlar, fındık yağının test edilen diğer bitkisel
yağlarla karşılaştırıldığında S.
clavuligerus ATCC27064'taki klavulanik asit üretimi üzerinde belirgin bir
üstünlük sağladığını göstermektedir.

Anahtar Kelimeler

Streptomyces clavuligerus, Klavulanik asit, Bitkisel yağ, Fındık yağı

The effect of different vegetable oils on clavulanic acid production in Streptomyces clavuligerus

Öz

In this study, it was
aimed to determine the effect of different vegetable oils as carbon source, on
the production of clavulanic acid in Streptomyces
clavuligerus ATCC27064. Cultures that were incubated at 28°C and 220 rpm
for 168 hours in the presence of olive oil, corn oil, sunflower oil and
hazelnut oil in the fermentation media were found to have better growth pattern
than control. According to agar plate diffusion results, the use of sunflower
and olive oil resulted in lower clavulanic acid production in S. clavuligerus ATCC27064 in comparison
to the control. The highest clavulanic acid production (1526.4 mg L^-1) was obtained at 120 hours (T₁₂₀) of
incubation due to use of hazelnut oil. The results showed that hazelnut oil
exerted a remarkable superiority over the tested vegetable oils in clavulanic
acid production in S. clavuligerus
ATCC27064

Anahtar Kelimeler

Streptomyces clavuligerus, ATCC27064, Clavulanic acid, Vegetable oil, Hazelnut oil, Agar diffusion method

Kaynakça

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