Fasulye üretim alanlarında hastalığa neden olan Xanthomonas axonopodis pv. phaseoli ve Pseudomonas savastonoi pv. phaseolicola’nın izolasyonu ve tanılanması

Yıl 2018, Cilt: 33 Sayı: 2, 105 - 115, 22.06.2018

Murat Ozturk Hasan Murat Aksoy

https://doi.org/10.7161/omuanajas.393903

Cited By: 1

Öz

2013-2014 yıllarında, Samsun ili fasulye üretim
alanlarında adi yanıklığa neden olan Xanthomonas
axonopodis pv. phaseoli (Xap) ve hale
yanıklığına neden olan Pseudomonas savastonoi pv. phaseolicola (Psp)’nin
belirlenmesi için surveyler yapılmıştır. Elde edilen tek ve saf kolonilerle
yapılan biyokimyasal, patojenisite, moleküler ve 16S rDNA sekanslama
çalışmaları sonucunda 8 Xap ve 5 Psp izolatı tanılanmıştır. Xap ve Psp izolatları filogenetik ağaç analizinde tip kültürleri ile birlikte
yer almıştır. Göynük 98 fasulye çeşidi in vitro patojenisite denemelerinde en
hassas çeşit olarak belirlenmiştir ve virülens denemesi için kullanılmıştır.
Yapılan bu çalışmada, Türkiye taze fasulye üretiminin % 20’sinin gerçekleştiği
Samsun ilinde her iki hastalık etmenin varlığı moleküler çalışmalarla ilk kez
ortaya konmuştur.

Anahtar Kelimeler

Adi yanıklık, Hale yanıklığı, PCR, 16SrDNA

Isolation and identification of Xanthomonas axonopodis pv. phaseoli and Pseudomonas savastonoi pv. phaseolicola causing disease in common bean producing areas

Öz

In 2013-2014, surveys were conducted in order to
investigate the presence of Xanthomonas
axonopodis pv. phaseoli (Xap) (common
blight) and Pseudomonas savastonoi pv. phaseolicola (Psp) (halo
blight) in common bean producing areas of Samsun province. Eight Xap and 5 Psp isolates were identified from single isolated pure colonies of
the bacteria by means of biochemical, pathogenicity, molecular and 16S rDNA
sequencing studies. Phylogenetic tree analysis demonstrated that the isolates
of Xap and Psp obtained were clustered with their type strains. Göynük 98 was
the most susceptible cultivar to Xap
and Psp isolates among the five
common bean cultivars tested in pathogenicity assays and selected for virulence
assays. This work proved the presence of both disease agents in Samsun
province, which supplies about 20 % of the total fresh bean production in
Turkey, with molecular studies for the first time.

Anahtar Kelimeler

Common blight, Halo blight, PCR, 16SrDNA

Kaynakça

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