TY  - JOUR
TT  - The Resistance State of Campylobacter jejuni Isolated from Broiler Carcasses Against to Macrolide, Quinolone and Tetracycline Groups of Antibiotics
AU  - Hızlısoy, Harun
AU  - Kılıç, Hüseyin
PY  - 2015
DA  - July
Y2  - 2015
JF  - Erciyes Üniversitesi Veteriner Fakültesi Dergisi
JO  - Erciyes Üniv Vet Fak Derg
PB  - Erciyes Üniversitesi
WT  - DergiPark
SN  - 1304-7280
SP  - 81
EP  - 92
VL  - 12
IS  - 2
KW  - Broiler
KW  - Campylobacter jejuni
KW  - kinolon
KW  - makrolid
KW  - tetrasiklin
N2  - : In the study, in June 2012 and in January 2013, the isolation of Campylobacter jejuni from a total of 200 broiler carcassesin different sales points in the city center of Kayseri and the determination of resistance states to macrolides, quinoloneand tetracycline groups of antibiotics by using phenotypical and genotypical methods were aimed.Two hundered samples including chicken thigh, breast, wings and carcasses were examined in the study. The identification ofthe isolates obtained from samples were performed by phenotypical tests and multiplex Polymerase Chain Reaction (mPCR) method. The antibiotic resistance and MIC values of C. jejuni isolates were detected with disk diffusion and E test method,respectively. In addition, the antibiotic resistances were tested by using of PCR and sequencing.Eighty-one of 100 samples collected in June and 77 of 100 samples in January were found to be positive in terms of Campylobacterspp. As a result of mPCR, 88 of 124 and 68 of 109 Campylobacter spp. isolates were identified as C. jejuni from duringJune and January terms, respectively. In disk diffusion test, while, the highest resistance rates were observed as nalidixic acid(78.4% - 92.6%), ciprofloxacin (67.1% - 86.7%) and tetracycline (55.6% - 64.7%), the lowest resistance rates as azithromycin(1.1% - 4.4%), doxycycline (2.2% - 1.4%) and erythromycin (11.3% - 4.4% ), for the period June and January, were respectivelyfound. It was determined that E test results were compatible with disk diffusion test. Whilst, chromosomal tetO gene wasshown in June and January isolates at 88 % and 85.7 % rates, plasmid mediated tetO gene was found at 93.7% and 85.7%rates, respectively. In macrolide resistance, while A2075G mutation was detected in 23SrRNA at rates 18.4% and 14.8% ofJune and January isolates, A2074C mutation was not observed at any of June or January isolates, respectively. When, in chromosomalquinolone resistance, the mutations in gyrA gene were detected at rates of 83.5% and 81.8% in June and Januaryisolates, respectively, qnr gene examined for plasmid mediated quinolone resistance was found in none of C. jejuni isolates.Quinolone and macrolide resistances detected by PCR were confirmed by DNA sequencing. While, Tre-86-Ile mutation wasfound in quinolone resistant isolates with sequencing, but these mutations were not detected in susceptible isolates. Additionally,A2075G mutation was detected in macrolide resistant isolates. However, A2074C mutation was observed in any isolatesby using sequencing. It was not observed that none of the macrolide susceptible isolates had any of these two mutations. Inconclusion, due to the presence of antibiotic resistant C. jejuni detected in chicken meat, protective measures should be takenfor, the using of narrow-spectrum antibiotics may be preferred, animals treated with antibiotics, before the completion of withdrawalperiod of antibiotics from organisms, animals may not be slaughtered or supplied for consumption. Besides, it is consideredthat in the treatment, azythromycin and doxycycline may be used as alternate to erythromycin in the drug of choice.
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