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Gingivitisli Hastalarda Cerrahi Olmayan Periodontal Tedavinin Tükürük PTX-3 Seviyesine Etkisinin Değerlendirilmesi

Yıl 2023, Cilt: 9 Sayı: 1, 1 - 14, 27.04.2023

Öz

Amaç: Akut faz proteinleri ailesinin bir üyesi olan pentraksin-3 (PTX-3), çeşitli sitokinlerin etkisi ile endotel hücreleri, fibroblastlar, makrofajlar ve nötrofiller gibi enflamasyon bölgesinde bulunan hücrelerden salınmaktadır. Sağlıklı bireylerde düşük olduğu bilinen plazma PTX-3 konsantrasyonları kronik enflamatuvar hastalıklarda artış göstermektedir. Bu çalışmada gingivitisli hastalarda cerrahi olmayan periodontal tedavinin (COPT) tükürük PTX-3 seviyeleri üzerine etkisinin değerlendirilmesi amaçlandı.
Gereç ve Yöntem: Çalışmaya 13 gingivitisli hasta (gingivitis grubu) ve 13 periodontal sağlıklı birey (kontrol grubu) olmak üzere toplam 26 birey dahil edildi. Çalışmanın başlangıcında tüm bireylerden plak indeks, gingival indeks, sondalamada kanama ve sondalama derinliğinden oluşan periodontal klinik parametreler ölçülerek tükürük örnekleri toplandı. Gingivitis grubuna COPT uygulandı. COPT sonrası 3. ayda gingivitis grubunda klinik ölçümler ve tükürük örneklerinin toplanması tekrarlandı. Tükürük PTX-3 seviyeleri ELISA yöntemiyle saptandı.
Bulgular: Gingivitis grubunda tüm periodontal klinik parametrelerin başlangıçta kontrol grubuna kıyasla daha yüksek olduğu (p<0,001) ve COPT sonrası istatistiksel anlamlı azalma gösterdiği saptandı (p<0,01). Başlangıçta gingivitis grubunda tükürük PTX-3 seviyesi kontrol grubuna kıyasla anlamlı derecede yüksek bulundu (p<0,05). Gingivitis grubunda COPT sonrası tükürük PTX-3 seviyesi tedavi öncesine kıyasla azalmakla birlikte aralarındaki fark anlamlı bulunmadı (p>0,05).
Sonuç: PTX-3’ün kronik enflamatuvar bir hastalık olan gingivitisin patogenezinde rolü olabileceği gösterilmiş olmakla birlikte COPT’nin tükürük PTX-3 seviyelerine etkisinin açıklığa kavuşturulabilmesi için daha fazla çalışmaya ihtiyaç olduğu sonucuna varılmıştır.

Kaynakça

  • 1. Gabay C, Kushner I. Acute-phase proteins and other systemic responses to inflammation. N Engl J Med. 1999;340(6):448-54.
  • 2. Yücel AE. C-Reaktif Protein (CRP) ve Diğer Akut Faz Proteinlerinin Klinik Kullanımı. Türkiye Tıp Dergisi. 2004;11(1):42-52.
  • 3. Moshage H. Cytokines and the hepatic acute phase response. J Pathol. 1997;181(3):257-66.
  • 4. Günaştı N, Balcı Ş, Tamer L. Pentraksinler. Mersin Üniversitesi Sağlık Bilimleri Dergisi. 2017;10(1):65-71.
  • 5. Lee GW, Lee TH, Vilcek J. TSG-14, a tumor necrosis factor-and IL-1-inducible protein, is a novel member of the pentaxin family of acute phase proteins. J Immunol. 1993;150(5):1804-12.
  • 6. Breviario F, d'Aniello EM, Golay J, Peri G, Bottazzi B, Bairoch A, ve ark. Interleukin-1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component. J Biol Chem. 1992;267(31):22190-7.
  • 7. Fazzini F, Peri G, Doni A, Dell'Antonio G, Cin ED, Bozzolo E, ve ark. PTX3 in small‐vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation. Arthritis Rheumatol. 2001;44(12):2841-50.
  • 8. Mantovani A, Garlanda C, Doni A, Bottazzi B. Pentraxins in innate immunity: from C-reactive protein to the long pentraxin PTX3. J Clin Immunol. 2008;28(1):1-13.
  • 9. Okutani D. The role of long pentraxin 3, a new inflammatory mediator in inflammatory responses. Nihon Rinsho. 2006;29(3):107-13.
  • 10. Gümüş P, Nizam N, Nalbantsoy A, Özçaka Ö, Buduneli N. Saliva and serum levels of pentraxin‐3 and interleukin‐1β in generalized aggressive or chronic periodontitis. J Periodontol. 2014;85(3):e40-e6.
  • 11. Pradeep A, Kathariya R, Raghavendra N, Sharma A. Levels of pentraxin‐3 in gingival crevicular fluid and plasma in periodontal health and disease. J Periodontol. 2011;82(5):734-41.
  • 12. Fujita Y, Ito H, Sekino S, Numabe Y. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology. 2012;100(2):215-21.
  • 13. Mohan R, Varghese J, Bhat V, Chianeh YR. The effect of nonsurgical periodontal therapy on pentraxin 3 levels in smokers and nonsmokers with chronic periodontitis. Gen Dent. 2019;67(2):e1-e6.
  • 14. Tasdemir Z, Oskaybas MN, Alkan AB, Cakmak O. The effects of ozone therapy on periodontal therapy: A randomized placebo‐controlled clinical trial. Oral Dis. 2019;25(4):1195-202.
  • 15. Yarkac FU, Gokturk O, Demir O. Effect of non-surgical periodontal therapy on the degree of gingival inflammation and stress markers related to pregnancy. J Appl Oral Sci. 2018;26.
  • 16. Chapple IL, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, ve ark. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri‐Implant Diseases and Conditions. J Periodontol. 2018;89:S74-S84.
  • 17. Silness J, Löe H. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand. 1964;22(1):121-35.
  • 18. Löe H, Silness J. Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontol Scand. 1963;21(6):533-51.
  • 19. Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J.1975;25(4):229-35.
  • 20. Bass C. An effective method of personal oral hygiene. J La State Med Soc 1954;106(2):57-73.
  • 21. Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;694(1):72-7.
  • 22. Kaufman E, Lamster IB. Analysis of saliva for periodontal diagnosis: a review. J Clin Periodontol. 2000;27(7):453-65.
  • 23. Adriaens PA, Adriaens LM. Effects of nonsurgical periodontal therapy on hard and soft tissues. Periodontol 2000. 2004;36(1):121-45.
  • 24. Beiler TF, de Mello Neto JM, Alves JC, Hamlet S, Ipe D, da Silva Figueredo CM. Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism. Odontology. 2020;108(4):646-52.
  • 25. Arslan R, Karsiyaka Hendek M, Kisa U, Olgun E. The effect of non‐surgical periodontal treatment on gingival crevicular fluid periostin levels in patients with gingivitis and periodontitis. Oral Diseases. 2021;27(6):1478-86.
  • 26. Alreja D, Rao JR, Kataria S, Faterpenkar DA. Effect of Nonsurgical Treatment on Salivary HGF Levels in Population with Periodontal Disease: A Quasi-experimental Study. Euroasian J Hepatogastroenterol 2020;10(2):51.
  • 27. Keles GC, Balli U, Cetinkaya BO, Ayas B, Findik A, Keles ZP, ve ark. Biochemical analysis of pentraxin 3 and fibrinogen levels in experimental periodontitis model. Mediators Inflamm. 2012;2012.
  • 28. Lakshmanan R, Jayakumar N, Sankari M, Padmalatha O, Varghese S. Estimation of Pentraxin‐3 Levels in the Gingival Tissues of Chronic and Aggressive Periodontitis Participants: An In Vivo Study. J Periodontol. 2014;85(2):290-7.
  • 29. Temelli B, Yetkin Ay Z, Savaş HB, Aksoy F, Kumbul Doğuç D, Uskun E, ve ark. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. J Appl Oral Sci. 2018;26.
  • 30. Pradeep A, Kathariya R, Arjun Raju P, Sushma Rani R, Sharma A, Raghavendra N. Risk factors for chronic kidney diseases may include periodontal diseases, as estimated by the correlations of plasma pentraxin-3 levels: a case–control study. Int Urol Nephrol 2012;44(3):829-39. 31. Leira Y, Ameijeira P, Domínguez C, López-Arias E, Ávila-Gómez P, Pérez-Mato M, ve ark. Severe periodontitis is linked with increased peripheral levels of sTWEAK and PTX3 in chronic migraineurs. Clin Oral Investig. 2020;24(2):597-606.
  • 32. Parmar M, Patel S, Parmar M, Shah F, Patel B, Joshi J. Comparison of Level of Pentraxin-3 in Gingival Crevicular Fluid with Chronic Periodontitis in Well Controlled and Uncontrolled Diabetes Mellitus Patients. Natl J Integr Res Med. 2018;9(2).
  • 33. Bosutti A, Malaponte G, Zanetti M, Castellino P, Heer M, Guarnieri G, ve ark. Calorie restriction modulates inactivity-induced changes in the inflammatory markers C-reactive protein and pentraxin-3. J Clin Endocrinol Metab. 2008;93(8):3226-9.

Effect of Non-Surgical Periodontal Treatment On Salivary PTX-3 Level In Gingivitis Patients

Yıl 2023, Cilt: 9 Sayı: 1, 1 - 14, 27.04.2023

Öz

Objectives: Pentraxin-3 (PTX-3), a member of the acute phase protein family, is released from cells such as endothelial cells, fibroblasts, macrophages, and neutrophils induced by cytokines in the inflammation region. Plasma PTX-3 concentrations, known to be low in healthy individuals, increase in chronic inflammatory diseases. This study aimed to evaluate the effect of non-surgical periodontal therapy (NSPT) on salivary PTX-3 levels in patients with gingivitis.
Material and Methods: A total of 26 subjects, including 13 gingivitis patients (gingivitis group), and 13 periodontally healthy individuals (control group), were included in the study. At the beginning of the study, saliva samples were collected, and periodontal clinical parameters including plaque index, gingival index, bleeding on probing, and probing depth were measured from all individuals. NSPT was applied to the gingivitis group. Clinical measurements and sample collection were repeated in the gingivitis group 3 months after NSPT. PTX-3 levels in saliva were determined by ELISA method.
Result: All periodontal clinical parameters were found to be higher in the gingivitis group compared to the control group at baseline (p<0,001), and showed a statistically significant decrease after NSPT (p<0,01). Salivary PTX-3 level was significantly higher in the gingivitis group than the control group at baseline (p<0,05). Although salivary PTX-3 level decreased after NSPT in the gingivitis group, there was no significant difference between baseline and after 3 months after NSPT (p>0,05).
Conclusions: It was demonstrated that PTX-3 may play a role in the pathogenesis of gingivitis. However, it was concluded that further studies are needed to clarify the effect of NSPT on salivary PTX-3 level.

Kaynakça

  • 1. Gabay C, Kushner I. Acute-phase proteins and other systemic responses to inflammation. N Engl J Med. 1999;340(6):448-54.
  • 2. Yücel AE. C-Reaktif Protein (CRP) ve Diğer Akut Faz Proteinlerinin Klinik Kullanımı. Türkiye Tıp Dergisi. 2004;11(1):42-52.
  • 3. Moshage H. Cytokines and the hepatic acute phase response. J Pathol. 1997;181(3):257-66.
  • 4. Günaştı N, Balcı Ş, Tamer L. Pentraksinler. Mersin Üniversitesi Sağlık Bilimleri Dergisi. 2017;10(1):65-71.
  • 5. Lee GW, Lee TH, Vilcek J. TSG-14, a tumor necrosis factor-and IL-1-inducible protein, is a novel member of the pentaxin family of acute phase proteins. J Immunol. 1993;150(5):1804-12.
  • 6. Breviario F, d'Aniello EM, Golay J, Peri G, Bottazzi B, Bairoch A, ve ark. Interleukin-1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component. J Biol Chem. 1992;267(31):22190-7.
  • 7. Fazzini F, Peri G, Doni A, Dell'Antonio G, Cin ED, Bozzolo E, ve ark. PTX3 in small‐vessel vasculitides: an independent indicator of disease activity produced at sites of inflammation. Arthritis Rheumatol. 2001;44(12):2841-50.
  • 8. Mantovani A, Garlanda C, Doni A, Bottazzi B. Pentraxins in innate immunity: from C-reactive protein to the long pentraxin PTX3. J Clin Immunol. 2008;28(1):1-13.
  • 9. Okutani D. The role of long pentraxin 3, a new inflammatory mediator in inflammatory responses. Nihon Rinsho. 2006;29(3):107-13.
  • 10. Gümüş P, Nizam N, Nalbantsoy A, Özçaka Ö, Buduneli N. Saliva and serum levels of pentraxin‐3 and interleukin‐1β in generalized aggressive or chronic periodontitis. J Periodontol. 2014;85(3):e40-e6.
  • 11. Pradeep A, Kathariya R, Raghavendra N, Sharma A. Levels of pentraxin‐3 in gingival crevicular fluid and plasma in periodontal health and disease. J Periodontol. 2011;82(5):734-41.
  • 12. Fujita Y, Ito H, Sekino S, Numabe Y. Correlations between pentraxin 3 or cytokine levels in gingival crevicular fluid and clinical parameters of chronic periodontitis. Odontology. 2012;100(2):215-21.
  • 13. Mohan R, Varghese J, Bhat V, Chianeh YR. The effect of nonsurgical periodontal therapy on pentraxin 3 levels in smokers and nonsmokers with chronic periodontitis. Gen Dent. 2019;67(2):e1-e6.
  • 14. Tasdemir Z, Oskaybas MN, Alkan AB, Cakmak O. The effects of ozone therapy on periodontal therapy: A randomized placebo‐controlled clinical trial. Oral Dis. 2019;25(4):1195-202.
  • 15. Yarkac FU, Gokturk O, Demir O. Effect of non-surgical periodontal therapy on the degree of gingival inflammation and stress markers related to pregnancy. J Appl Oral Sci. 2018;26.
  • 16. Chapple IL, Mealey BL, Van Dyke TE, Bartold PM, Dommisch H, Eickholz P, ve ark. Periodontal health and gingival diseases and conditions on an intact and a reduced periodontium: Consensus report of workgroup 1 of the 2017 World Workshop on the Classification of Periodontal and Peri‐Implant Diseases and Conditions. J Periodontol. 2018;89:S74-S84.
  • 17. Silness J, Löe H. Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand. 1964;22(1):121-35.
  • 18. Löe H, Silness J. Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontol Scand. 1963;21(6):533-51.
  • 19. Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J.1975;25(4):229-35.
  • 20. Bass C. An effective method of personal oral hygiene. J La State Med Soc 1954;106(2):57-73.
  • 21. Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;694(1):72-7.
  • 22. Kaufman E, Lamster IB. Analysis of saliva for periodontal diagnosis: a review. J Clin Periodontol. 2000;27(7):453-65.
  • 23. Adriaens PA, Adriaens LM. Effects of nonsurgical periodontal therapy on hard and soft tissues. Periodontol 2000. 2004;36(1):121-45.
  • 24. Beiler TF, de Mello Neto JM, Alves JC, Hamlet S, Ipe D, da Silva Figueredo CM. Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism. Odontology. 2020;108(4):646-52.
  • 25. Arslan R, Karsiyaka Hendek M, Kisa U, Olgun E. The effect of non‐surgical periodontal treatment on gingival crevicular fluid periostin levels in patients with gingivitis and periodontitis. Oral Diseases. 2021;27(6):1478-86.
  • 26. Alreja D, Rao JR, Kataria S, Faterpenkar DA. Effect of Nonsurgical Treatment on Salivary HGF Levels in Population with Periodontal Disease: A Quasi-experimental Study. Euroasian J Hepatogastroenterol 2020;10(2):51.
  • 27. Keles GC, Balli U, Cetinkaya BO, Ayas B, Findik A, Keles ZP, ve ark. Biochemical analysis of pentraxin 3 and fibrinogen levels in experimental periodontitis model. Mediators Inflamm. 2012;2012.
  • 28. Lakshmanan R, Jayakumar N, Sankari M, Padmalatha O, Varghese S. Estimation of Pentraxin‐3 Levels in the Gingival Tissues of Chronic and Aggressive Periodontitis Participants: An In Vivo Study. J Periodontol. 2014;85(2):290-7.
  • 29. Temelli B, Yetkin Ay Z, Savaş HB, Aksoy F, Kumbul Doğuç D, Uskun E, ve ark. Circulation levels of acute phase proteins pentraxin 3 and serum amyloid A in atherosclerosis have correlations with periodontal inflamed surface area. J Appl Oral Sci. 2018;26.
  • 30. Pradeep A, Kathariya R, Arjun Raju P, Sushma Rani R, Sharma A, Raghavendra N. Risk factors for chronic kidney diseases may include periodontal diseases, as estimated by the correlations of plasma pentraxin-3 levels: a case–control study. Int Urol Nephrol 2012;44(3):829-39. 31. Leira Y, Ameijeira P, Domínguez C, López-Arias E, Ávila-Gómez P, Pérez-Mato M, ve ark. Severe periodontitis is linked with increased peripheral levels of sTWEAK and PTX3 in chronic migraineurs. Clin Oral Investig. 2020;24(2):597-606.
  • 32. Parmar M, Patel S, Parmar M, Shah F, Patel B, Joshi J. Comparison of Level of Pentraxin-3 in Gingival Crevicular Fluid with Chronic Periodontitis in Well Controlled and Uncontrolled Diabetes Mellitus Patients. Natl J Integr Res Med. 2018;9(2).
  • 33. Bosutti A, Malaponte G, Zanetti M, Castellino P, Heer M, Guarnieri G, ve ark. Calorie restriction modulates inactivity-induced changes in the inflammatory markers C-reactive protein and pentraxin-3. J Clin Endocrinol Metab. 2008;93(8):3226-9.
Toplam 32 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makalesi
Yazarlar

Ece Taşkın Baş 0000-0001-9812-2580

Kübra Kundak 0000-0001-5979-7358

Başak Doğan 0000-0002-3602-4886

Leyla Kuru 0000-0002-7599-2409

Yayımlanma Tarihi 27 Nisan 2023
Gönderilme Tarihi 3 Ekim 2022
Yayımlandığı Sayı Yıl 2023 Cilt: 9 Sayı: 1

Kaynak Göster

Vancouver Taşkın Baş E, Kundak K, Doğan B, Kuru L. Gingivitisli Hastalarda Cerrahi Olmayan Periodontal Tedavinin Tükürük PTX-3 Seviyesine Etkisinin Değerlendirilmesi. Aydin Dental Journal. 2023;9(1):1-14.

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