Araştırma Makalesi
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Yıl 2023, Cilt: 1 Sayı: 2, 57 - 64, 30.11.2023

Öz

Proje Numarası

1504

Kaynakça

  • P. H. Rampelotto, “Extremophiles and Extreme Environments,” Life, vol. 3, no. 3, pp. 482–485, Aug. 2013, doi:10.3390/life3030482.
  • L. Sawle and K. Ghosh, “How Do Thermophilic Proteins and Proteomes Withstand High Temperature?,” Biophys. J., vol. 101, no. 1, pp. 217–227, Jul. 2011, doi:10.1016/j.bpj.2011.05.059.
  • M. E. DeCastro, E. Rodriguez-Belmonte, and M. I. Gonzalez-Siso, “Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes,” Front. in Microbiol., vol. 7, 1521, Sept. 2016, doi:10.3389/fmicb.2016.01521.
  • J. Atalah, P. Cáceres-Moreno, G. Espina, and J. M. Blamey, “Thermophiles and the Applications of Their Enzymes as New Biocatalysts,” Bioresour. Technol., vol. 280, pp. 478–488, May. 2019, doi:10.1016/j.biortech.2019.02.008.
  • U. Serpen and R. DiPippo, “Turkey-A Geothermal Success Story: A Retrospective and Prospective Assessment,” Geothermics, vol. 101, 102370, May. 2022, doi:10.1016/j.geothermics.2022.102370.
  • Maden Tetkik ve Arama Genel Müdürlüğü (MTA), “Türkiye Jeotermal Enerji Potansiyeli ve Arama Çalışmaları,” https://www.mta.gov.tr/v3.0/arastirmalar/jeotermal-enerji-arastirmalari. [Access Date: 12-April-2023].
  • A. Adıgüzel, K. İnan, F. Şahin, T. Arasoğlu, M. Güllüce, A. O. Beldüz, and Z. Barış, “Molecular Diversity of Thermophilic Bacteria Isolated from Pasinler Hot Spring,” Turk. J. Biol., vol. 35, no. 3, pp. 267–274, Jan. 2011, doi:10.3906/biy-0908-7.
  • E. Celikoglu and M. Yilmaz Cankilic, “Prokaryotic Diversity of Hot Springs in Balikesir and Kütahya, Turkey,” Appl. Ecol. Environ. Res., vol. 14, no. 4, pp. 429–440, Sep. 2016, doi:10.15666/aeer/1404_429440.
  • K. Inan, A. Ozer, H. I. Güler, A. O. Belduz, and S. Canakci, “Brevibacillus gelatini sp. nov., Isolated from a Hot Spring,” Int. J. Syst. Evol., vol. 66, no. 2, pp. 712–718, Feb. 2016, doi:10.1099/ijsem.0.000780.
  • M. O. Baltaci, H. Ay, S. Akbulut, G. Adiguzel, S. Albayrak, M. A. Omeroglu, H. Ozkan, M. Taskın, and A. Adiguzel, “Bacillus pasinlerensis sp. nov., a Thermophilic Bacterium Isolated from a Hot Spring in Turkey,” Int. J. Syst. Evol. Microbiol., vol. 70, no. 6, pp. 3865–3871, Jun. 2020, doi:10.1099/ijsem.0.004246.
  • S. Çalışkan-Özdemir, S. Önal, and A. Uzel, “Partial Purification and Characterization of a Thermostable Phytase Produced by Thermotolerant Aspergillus tubingensis TEM 37 Isolated from Hot Spring Soil in Gediz Geothermal Field, Turkey,” Geomicrobiol. J., vol. 38, no. 10, pp. 895–904, Sept. 2021, doi:10.1080/01490451.2021.1977432.
  • F. Matpan Bekler, K. Güven, and R. Gül Güven, “Purification and Characterization of Novel α-Amylase from Anoxybacillus ayderensis FMB1,” Biocatal. Biotransformation, vol. 39, no. 4, pp. 322–332, Dec. 2020, doi:10.1080/10242422.2020.1856097.
  • B. Genc, H. Nadaroglu, A. Adiguzel, and O. Baltaci, “Purification and Characterization of an Extracellular Cellulase from Anoxybacillus gonensis O9 Isolated from Geothermal Area in Turkey,” J. Environ. Biol., vol. 36, no. 6, pp. 1319–1324, Nov. 2015.
  • I. Çelik and E. Keskin, “Revealing the Microbiome of Four Different Thermal Springs in Turkey with Environmental DNA Metabarcoding,” Biology, vol. 11, no. 7, 998, Jun. 2022, doi:10.3390/biology11070998.
  • A. Strazzulli, S. Fusco, B. Cobucci-Ponzano, M. Moracci, and P. Contursi, “Metagenomics of Microbial and Viral Life in Terrestrial Geothermal Environments,” Rev. Environ. Sci. Biotechnol., vol. 16, pp. 425–454, Jun. 2017, doi:10.1007/s11157-017-9435-0.
  • L. Uribe‐Lorío, L. Brenes-Guillén, W. Hernández‐Ascencio, R. Mora‐Amador, G. González, C. J. Ramírez‐Umaña, B. Díez, and C. Pedrós‐Alió, “The Influence of Temperature and pH on Bacterial Community Composition of Microbial Mats in Hot Springs from Costa Rica,” Microbiologyopen, vol. 8, no.10, e893, Oct. 2019, doi:10.1002/mbo3.893.
  • D. Peterson, K.S. Bonham, S. Rowland, C.W. Pattanayak, and V. Klepac-Ceraj, “Comparative Analysis of 16S rRNA Gene and Metagenome Sequencing in Pediatric Gut Microbiomes,” Front. Microbiol., vol. 12, Jul. 2021, doi:10.3389/fmicb.2021.670336.
  • K. F. Al, and J. P. Burton, “Processing Human Urine and Ureteral Stents for 16S rRNA Amplicon Sequencing,” STAR Protoc., vol. 2, no. 2, 100435, Jun. 2021, doi:10.1016/j.xpro.2021.100435.
  • N. An, C. Wang, X. Dou, X. Liu, J. Wu, and Y. Cheng, “Comparison of 16S rDNA Amplicon Sequencing with the Culture Method for Diagnosing Causative Pathogens in Bacterial Corneal Infections,” Transl. Vis. Sci. Technol., vol. 11, no. 2, 29, Feb. 2022, doi:10.1167/tvst.11.2.29.
  • O. López-López, M. E. Cerdán, and M. I. González-Siso, “Hot Spring Metagenomics,” Life, vol. 3, no. 2, pp. 308–320, Apr. 2013, doi:10.3390/life3020308.
  • M. Oztug, A. Cebeci, H. Mumcu, M. Akgoz, and N. G. Karaguler, “Whole-Genome Sequence of Geobacillus thermoleovorans ARTRW1, Isolated from Armutlu Geothermal Spring, Turkey,” Microbiol. Resour. Announc., vol. 9, no. 24, e00269-20, Jun. 2020, doi:10.1128/mra.00269-20.
  • Wasimuddin, K. Schlaeppi, F. Ronchi, S. L. Leib, M. Erb, and A. Ramette, “Evaluation of Primer Pairs for Microbiome Profiling from Soils to Humans within the One Health Framework,” Mol. Ecol. Resour., vol. 20, no. 6, pp. 1558–1571, Jun. 2020, doi:10.1111/1755-0998.13215.
  • M. Martin, “Cutadapt Removes Adapter Sequences from High-throughput Sequencing Reads,” EMBnet. j., vol. 17, no. 1, pp. 10–12, May. 2011, doi:10.14806/ej.17.1.200.
  • E. Bolyen, J. R. Rideout, M. R. Dillon, N. A. Bokulich, C. C. Abnet, G. A. Al-Ghalith, H. Alexander, E. J. Alm, M. Arumugam, F. Asnicar, Y. Bai, J. E. Bisanz, K. Bittinger, A. Brejnrod, C. J. Brislawn, C. T. Brown, B. J. Callahan, A. M. Caraballo-Rodríguez, J. Chase, E. K. Cope, … and J. G. Caporaso, “Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2,” Nat. Biotechnol., vol. 37, no. 8, pp. 852–857, Jul. 2019, doi:10.1038/s41587-019-0209-9.
  • A. Amir, D. McDonald, J. A. Navas-Molina, E. Kopylova, J. T. Morton, Z. Zech Xu, E. P. Kightley, L. R. Thompson, E. R. Hyde, A. Gonzalez, and R. Knight, “Deblur Rapidly Resolves Single-Nucleotide Community Sequence Patterns,” mSystems, vol. 2, no. 2, e00191-16, Mar. 2017, doi:10.1128/mSystems.00191-16.
  • T. Eisenlohr, “The Thermal Springs of the Armutlu Peninsula (NW Turkey) and their Relationship to Geology and Tectonic,” in Active Tectonics of Northwestern Anatolia – The MARMARA Poly-Project, vdf Hochschulverlag AG an der ETH Zurich, 1997, pp. 197-228.
  • B. Tunc, D. Caka, T. S. Irmak, H. Woith, S. Tunc, Ş. Bariş, M. F. Ozer, B. G. Lühr, E. Günther, H. Grosser, and J. Zschau, “The Armutlu Network: An Investigation into the Seismotectonic Setting of Armutlu–Yalova–Gemlik and the Surrounding Regions,” Ann. Geophys., vol. 54, no. 1, pp.35–45, Apr. 2011, doi:10.4401/ag-4877.
  • M. Sofía Urbieta, E. G. Toril, M. Alejandra Giaveno, A. A. Bazán, and E. R. Donati, “Archaeal and Bacterial Diversity in Five Different Hydrothermal Ponds in the Copahue Region in Argentina,” Syst. Appl. Microbiol., vol. 37, no. 6, pp. 429–441, Sept. 2014, doi:10.1016/j.syapm.2014.05.012.
  • B. Chaudhuri, T. Chowdhury, and B. Chattopadhyay, “Comparative Analysis of Microbial Diversity in Two Hot Springs of Bakreshwar, West Bengal, India,” Genom. Data., vol. 27, no. 12, pp. 122–129, Apr. 2017, doi:10.1016/j.gdata.2017.04.001.
  • M. Adjeroud, J. J. Escuder-Rodríguez, M. I. González-Siso and M. Kecha, “Metagenomic Investigation of Bacterial and Archaeal Diversity of Hammam Essalihine Hot Spring from Khenchela, Algeria,” Geomicrobiol. J., vol. 37, no. 9, pp. 804–817, Jun. 2020, doi:10.1080/01490451.2020.1783035.
  • A. Mashzhan, R. Javier-López, A. Kistaubayeva, I. Savitskaya, and N. K. Birkeland, “Metagenomics and Culture-Based Diversity Analysis of the Bacterial Community in the Zharkent Geothermal Spring in Kazakhstan,” Curr. Microbiol., vol. 78, no. 8, pp. 2926–2934, Aug. 2021, doi:10.1007/s00284-021-02545-2.
  • I. N. Najar, M. T. Sherpa, S. Das, S. Das, and N. Thakur, “Microbial Ecology of Two Hot Springs of Sikkim: Predominate Population and Geochemistry,” Sci. Total Environ., vol. 637–638, pp. 730–745, Oct. 2018, doi:10.1016/j.scitotenv.2018.05.037.
  • M. E. DeCastro, J. J. Escuder-Rodríguez, M. Becerra, E. Rodríguez-Belmonte, and M. I. González-Siso, “Comparative Metagenomic Analysis of Two Hot Springs from Ourense (Northwestern Spain) and Others Worldwide,” Front. Microbiol. vol. 26, no. 12, 769065, Nov. 2021. doi:10.3389/fmicb.2021.769065.
  • A. Amin, I. Ahmed, N. Salam, B. Y. Kim, D. Singh, X. Y. Zhi, M. Xiao, and W. J. Li, “Diversity and Distribution of Thermophilic Bacteria in Hot Springs of Pakistan,” Microb. Ecol., vol. 74 no. 1, pp. 116–127, Jan. 2017, doi:10.1007/s00248-017-0930-1.
  • A. Cox, E. L. Shock, and J. R. Having, “The Transition to Microbial Photosynthesis in Hot Spring Ecosystems,” Chem. Geol., vol. 280, no. 3–4, pp. 344–351, Jan. 2011, doi:10.1016/j.chemgeo.2010.11.022.
  • R. M. Kumar, K. Jani, J. R. Parvathi, B. M. Thomas, S. S. S. Raja, A. Pandey, and A. Sharma, “Bacterial Diversity of Geochemically Distinct Hot Springs Located in Maharashtra, India,” Arch. Microbiol., vol. 204, 110, Jan. 2022, doi:10.1007/s00203-021-02728-2.
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Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Yıl 2023, Cilt: 1 Sayı: 2, 57 - 64, 30.11.2023

Öz

The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 °C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria (98.36%) are the dominant taxa compared to Archaea (1.64%). Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria (42.49%), which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute ~87% of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

Destekleyen Kurum

ITU Scientific Research Project Coordination Unit

Proje Numarası

1504

Kaynakça

  • P. H. Rampelotto, “Extremophiles and Extreme Environments,” Life, vol. 3, no. 3, pp. 482–485, Aug. 2013, doi:10.3390/life3030482.
  • L. Sawle and K. Ghosh, “How Do Thermophilic Proteins and Proteomes Withstand High Temperature?,” Biophys. J., vol. 101, no. 1, pp. 217–227, Jul. 2011, doi:10.1016/j.bpj.2011.05.059.
  • M. E. DeCastro, E. Rodriguez-Belmonte, and M. I. Gonzalez-Siso, “Metagenomics of Thermophiles with a Focus on Discovery of Novel Thermozymes,” Front. in Microbiol., vol. 7, 1521, Sept. 2016, doi:10.3389/fmicb.2016.01521.
  • J. Atalah, P. Cáceres-Moreno, G. Espina, and J. M. Blamey, “Thermophiles and the Applications of Their Enzymes as New Biocatalysts,” Bioresour. Technol., vol. 280, pp. 478–488, May. 2019, doi:10.1016/j.biortech.2019.02.008.
  • U. Serpen and R. DiPippo, “Turkey-A Geothermal Success Story: A Retrospective and Prospective Assessment,” Geothermics, vol. 101, 102370, May. 2022, doi:10.1016/j.geothermics.2022.102370.
  • Maden Tetkik ve Arama Genel Müdürlüğü (MTA), “Türkiye Jeotermal Enerji Potansiyeli ve Arama Çalışmaları,” https://www.mta.gov.tr/v3.0/arastirmalar/jeotermal-enerji-arastirmalari. [Access Date: 12-April-2023].
  • A. Adıgüzel, K. İnan, F. Şahin, T. Arasoğlu, M. Güllüce, A. O. Beldüz, and Z. Barış, “Molecular Diversity of Thermophilic Bacteria Isolated from Pasinler Hot Spring,” Turk. J. Biol., vol. 35, no. 3, pp. 267–274, Jan. 2011, doi:10.3906/biy-0908-7.
  • E. Celikoglu and M. Yilmaz Cankilic, “Prokaryotic Diversity of Hot Springs in Balikesir and Kütahya, Turkey,” Appl. Ecol. Environ. Res., vol. 14, no. 4, pp. 429–440, Sep. 2016, doi:10.15666/aeer/1404_429440.
  • K. Inan, A. Ozer, H. I. Güler, A. O. Belduz, and S. Canakci, “Brevibacillus gelatini sp. nov., Isolated from a Hot Spring,” Int. J. Syst. Evol., vol. 66, no. 2, pp. 712–718, Feb. 2016, doi:10.1099/ijsem.0.000780.
  • M. O. Baltaci, H. Ay, S. Akbulut, G. Adiguzel, S. Albayrak, M. A. Omeroglu, H. Ozkan, M. Taskın, and A. Adiguzel, “Bacillus pasinlerensis sp. nov., a Thermophilic Bacterium Isolated from a Hot Spring in Turkey,” Int. J. Syst. Evol. Microbiol., vol. 70, no. 6, pp. 3865–3871, Jun. 2020, doi:10.1099/ijsem.0.004246.
  • S. Çalışkan-Özdemir, S. Önal, and A. Uzel, “Partial Purification and Characterization of a Thermostable Phytase Produced by Thermotolerant Aspergillus tubingensis TEM 37 Isolated from Hot Spring Soil in Gediz Geothermal Field, Turkey,” Geomicrobiol. J., vol. 38, no. 10, pp. 895–904, Sept. 2021, doi:10.1080/01490451.2021.1977432.
  • F. Matpan Bekler, K. Güven, and R. Gül Güven, “Purification and Characterization of Novel α-Amylase from Anoxybacillus ayderensis FMB1,” Biocatal. Biotransformation, vol. 39, no. 4, pp. 322–332, Dec. 2020, doi:10.1080/10242422.2020.1856097.
  • B. Genc, H. Nadaroglu, A. Adiguzel, and O. Baltaci, “Purification and Characterization of an Extracellular Cellulase from Anoxybacillus gonensis O9 Isolated from Geothermal Area in Turkey,” J. Environ. Biol., vol. 36, no. 6, pp. 1319–1324, Nov. 2015.
  • I. Çelik and E. Keskin, “Revealing the Microbiome of Four Different Thermal Springs in Turkey with Environmental DNA Metabarcoding,” Biology, vol. 11, no. 7, 998, Jun. 2022, doi:10.3390/biology11070998.
  • A. Strazzulli, S. Fusco, B. Cobucci-Ponzano, M. Moracci, and P. Contursi, “Metagenomics of Microbial and Viral Life in Terrestrial Geothermal Environments,” Rev. Environ. Sci. Biotechnol., vol. 16, pp. 425–454, Jun. 2017, doi:10.1007/s11157-017-9435-0.
  • L. Uribe‐Lorío, L. Brenes-Guillén, W. Hernández‐Ascencio, R. Mora‐Amador, G. González, C. J. Ramírez‐Umaña, B. Díez, and C. Pedrós‐Alió, “The Influence of Temperature and pH on Bacterial Community Composition of Microbial Mats in Hot Springs from Costa Rica,” Microbiologyopen, vol. 8, no.10, e893, Oct. 2019, doi:10.1002/mbo3.893.
  • D. Peterson, K.S. Bonham, S. Rowland, C.W. Pattanayak, and V. Klepac-Ceraj, “Comparative Analysis of 16S rRNA Gene and Metagenome Sequencing in Pediatric Gut Microbiomes,” Front. Microbiol., vol. 12, Jul. 2021, doi:10.3389/fmicb.2021.670336.
  • K. F. Al, and J. P. Burton, “Processing Human Urine and Ureteral Stents for 16S rRNA Amplicon Sequencing,” STAR Protoc., vol. 2, no. 2, 100435, Jun. 2021, doi:10.1016/j.xpro.2021.100435.
  • N. An, C. Wang, X. Dou, X. Liu, J. Wu, and Y. Cheng, “Comparison of 16S rDNA Amplicon Sequencing with the Culture Method for Diagnosing Causative Pathogens in Bacterial Corneal Infections,” Transl. Vis. Sci. Technol., vol. 11, no. 2, 29, Feb. 2022, doi:10.1167/tvst.11.2.29.
  • O. López-López, M. E. Cerdán, and M. I. González-Siso, “Hot Spring Metagenomics,” Life, vol. 3, no. 2, pp. 308–320, Apr. 2013, doi:10.3390/life3020308.
  • M. Oztug, A. Cebeci, H. Mumcu, M. Akgoz, and N. G. Karaguler, “Whole-Genome Sequence of Geobacillus thermoleovorans ARTRW1, Isolated from Armutlu Geothermal Spring, Turkey,” Microbiol. Resour. Announc., vol. 9, no. 24, e00269-20, Jun. 2020, doi:10.1128/mra.00269-20.
  • Wasimuddin, K. Schlaeppi, F. Ronchi, S. L. Leib, M. Erb, and A. Ramette, “Evaluation of Primer Pairs for Microbiome Profiling from Soils to Humans within the One Health Framework,” Mol. Ecol. Resour., vol. 20, no. 6, pp. 1558–1571, Jun. 2020, doi:10.1111/1755-0998.13215.
  • M. Martin, “Cutadapt Removes Adapter Sequences from High-throughput Sequencing Reads,” EMBnet. j., vol. 17, no. 1, pp. 10–12, May. 2011, doi:10.14806/ej.17.1.200.
  • E. Bolyen, J. R. Rideout, M. R. Dillon, N. A. Bokulich, C. C. Abnet, G. A. Al-Ghalith, H. Alexander, E. J. Alm, M. Arumugam, F. Asnicar, Y. Bai, J. E. Bisanz, K. Bittinger, A. Brejnrod, C. J. Brislawn, C. T. Brown, B. J. Callahan, A. M. Caraballo-Rodríguez, J. Chase, E. K. Cope, … and J. G. Caporaso, “Reproducible, Interactive, Scalable and Extensible Microbiome Data Science Using QIIME 2,” Nat. Biotechnol., vol. 37, no. 8, pp. 852–857, Jul. 2019, doi:10.1038/s41587-019-0209-9.
  • A. Amir, D. McDonald, J. A. Navas-Molina, E. Kopylova, J. T. Morton, Z. Zech Xu, E. P. Kightley, L. R. Thompson, E. R. Hyde, A. Gonzalez, and R. Knight, “Deblur Rapidly Resolves Single-Nucleotide Community Sequence Patterns,” mSystems, vol. 2, no. 2, e00191-16, Mar. 2017, doi:10.1128/mSystems.00191-16.
  • T. Eisenlohr, “The Thermal Springs of the Armutlu Peninsula (NW Turkey) and their Relationship to Geology and Tectonic,” in Active Tectonics of Northwestern Anatolia – The MARMARA Poly-Project, vdf Hochschulverlag AG an der ETH Zurich, 1997, pp. 197-228.
  • B. Tunc, D. Caka, T. S. Irmak, H. Woith, S. Tunc, Ş. Bariş, M. F. Ozer, B. G. Lühr, E. Günther, H. Grosser, and J. Zschau, “The Armutlu Network: An Investigation into the Seismotectonic Setting of Armutlu–Yalova–Gemlik and the Surrounding Regions,” Ann. Geophys., vol. 54, no. 1, pp.35–45, Apr. 2011, doi:10.4401/ag-4877.
  • M. Sofía Urbieta, E. G. Toril, M. Alejandra Giaveno, A. A. Bazán, and E. R. Donati, “Archaeal and Bacterial Diversity in Five Different Hydrothermal Ponds in the Copahue Region in Argentina,” Syst. Appl. Microbiol., vol. 37, no. 6, pp. 429–441, Sept. 2014, doi:10.1016/j.syapm.2014.05.012.
  • B. Chaudhuri, T. Chowdhury, and B. Chattopadhyay, “Comparative Analysis of Microbial Diversity in Two Hot Springs of Bakreshwar, West Bengal, India,” Genom. Data., vol. 27, no. 12, pp. 122–129, Apr. 2017, doi:10.1016/j.gdata.2017.04.001.
  • M. Adjeroud, J. J. Escuder-Rodríguez, M. I. González-Siso and M. Kecha, “Metagenomic Investigation of Bacterial and Archaeal Diversity of Hammam Essalihine Hot Spring from Khenchela, Algeria,” Geomicrobiol. J., vol. 37, no. 9, pp. 804–817, Jun. 2020, doi:10.1080/01490451.2020.1783035.
  • A. Mashzhan, R. Javier-López, A. Kistaubayeva, I. Savitskaya, and N. K. Birkeland, “Metagenomics and Culture-Based Diversity Analysis of the Bacterial Community in the Zharkent Geothermal Spring in Kazakhstan,” Curr. Microbiol., vol. 78, no. 8, pp. 2926–2934, Aug. 2021, doi:10.1007/s00284-021-02545-2.
  • I. N. Najar, M. T. Sherpa, S. Das, S. Das, and N. Thakur, “Microbial Ecology of Two Hot Springs of Sikkim: Predominate Population and Geochemistry,” Sci. Total Environ., vol. 637–638, pp. 730–745, Oct. 2018, doi:10.1016/j.scitotenv.2018.05.037.
  • M. E. DeCastro, J. J. Escuder-Rodríguez, M. Becerra, E. Rodríguez-Belmonte, and M. I. González-Siso, “Comparative Metagenomic Analysis of Two Hot Springs from Ourense (Northwestern Spain) and Others Worldwide,” Front. Microbiol. vol. 26, no. 12, 769065, Nov. 2021. doi:10.3389/fmicb.2021.769065.
  • A. Amin, I. Ahmed, N. Salam, B. Y. Kim, D. Singh, X. Y. Zhi, M. Xiao, and W. J. Li, “Diversity and Distribution of Thermophilic Bacteria in Hot Springs of Pakistan,” Microb. Ecol., vol. 74 no. 1, pp. 116–127, Jan. 2017, doi:10.1007/s00248-017-0930-1.
  • A. Cox, E. L. Shock, and J. R. Having, “The Transition to Microbial Photosynthesis in Hot Spring Ecosystems,” Chem. Geol., vol. 280, no. 3–4, pp. 344–351, Jan. 2011, doi:10.1016/j.chemgeo.2010.11.022.
  • R. M. Kumar, K. Jani, J. R. Parvathi, B. M. Thomas, S. S. S. Raja, A. Pandey, and A. Sharma, “Bacterial Diversity of Geochemically Distinct Hot Springs Located in Maharashtra, India,” Arch. Microbiol., vol. 204, 110, Jan. 2022, doi:10.1007/s00203-021-02728-2.
  • I. Verbaendert, N. Boon, P. De Vos, and K. Heylen, “Denitrification is a Common Feature among Members of the Genus Bacillus,” Syst. Appl. Microbiol., vol. 34, no. 5, pp. 385–391, Jul. 2011, doi:10.1016/j.syapm.2011.02.003.
  • K. Wasmund, M. Cooper, L. Schreiber, K. G. Lloyd, B. J. Baker, D. G. Petersen, B. B. Jørgensen, R. Stepanauskas, R. Reinhardt, A. Schramm, A. Loy, and L. Adrian, “Single-Cell Genome and Group-Specific dsrAB Sequencing Implicate Marine Members of the Class Dehalococcoidia (Phylum Chloroflexi) in Sulfur Cycling,” mBio., vol. 7, no. 3, e00266-16, May. 2016, doi:10.1128/mBio.00266-16.
  • J. Chen, Y. Liu, P. Diep, and R. Mahadevan, “Genomic Analysis of a Newly Isolated Acidithiobacillus ferridurans JAGS Strain Reveals its Adaptation to Acid Mine Drainage,” Minerals, vol. 11, no. 1, 74, Jan. 2021, doi:10.3390/min11010074.
  • S. Kumar, A. K. Dangi, P. Shukla, D. Baishya, and S. K. Khare, “Thermozymes: Adaptive Strategies and Tools for Their Biotechnological Applications,” Bioresour. Technol., vol. 278, pp. 372–382, Apr. 2019, doi:10.1016/j.biortech.2019.01.088.
Toplam 40 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Yapısal Biyoloji
Bölüm Research Articles
Yazarlar

Anıl Cebeci 0000-0001-6493-7714

Merve Öztuğ 0000-0002-6287-2774

Hande Mumcu 0000-0003-0270-6229

Nevin Gül Karagüler 0000-0002-2300-9484

Proje Numarası 1504
Yayımlanma Tarihi 30 Kasım 2023
Gönderilme Tarihi 25 Mayıs 2023
Yayımlandığı Sayı Yıl 2023 Cilt: 1 Sayı: 2

Kaynak Göster

IEEE A. Cebeci, M. Öztuğ, H. Mumcu, ve N. Gül Karagüler, “Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach”, Bozok J. Sci., c. 1, sy. 2, ss. 57–64, 2023.