Determination of TLX/ PstI polymorphism in Japanese quail

Yıl 2023, Cilt: 2 Sayı: 3, 1 - 4, 31.12.2023

Mervan Bayraktar Mikail Baylan B. Devrim Özcan Kadriye Kurşun Elif Dikkaya Gökhan Tamer Kayaalp

Öz

Abstract
The T-cell leukemia homeobox protein (TLX) gene is essential in vertebrate brain development and cognition. Polymorphisms in TLX can influence productive traits in quail. This study analyzed TLX gene variation in Japanese quail (Coturnix japonica). Blood samples were collected from 100 quails. The 544 bp of the TLX gene was amplified by PCR. The PCR-RFLP methods were used for genotyping. The PCR product was digested with PstI and three genotypes were obtained. AA (544 bp), AB (544, 402 and 142 bp) and BB (402 and 142 bp). Allele frequencies were 0.60 (A) and 0.40 (B). Genotype frequencies were 0.50 (AA), 0.19 (AB) and 0.31 (BB). The observed genotype proportions were in Hardy-Weinberg equilibrium. These results demonstrate polymorphism in the TLX gene within this Japanese quail flock. The genetic variability can be utilized in future studies to uncover potential associations between TLX genotypes and phenotypic traits related to cognition, learning, and behavior in quail. This foundational data will inform selective breeding and genome editing strategies targeting the TLX gene.

Anahtar Kelimeler

Coturnix japonica, TLX, Polymorphism, Allele frequency

Destekleyen Kurum

Bilimsel Araştırma Projeleri Birimi BAP

Proje Numarası

FBA-2021-14006

Kaynakça

• [1] Minvielle, F. (2004). The future of Japanese quail for research and production. Worlds Poult Sci J; 60:500-507.
• [2] Kayang, B.B., Vignal, A., Inoue-Murayama, M., Miwa, M., Monvoisin, J.L., Ito, S., & Minvielle, F. (2004). A first-generation microsatellite linkage map of the Japanese quail. Anim. Genet; 35:195-200.
• [3] Narinc, D., Karaman, E., Firat, M.Z., & Aksoy, T. (2013). Genetic parameters of growth curve parameters and weekly body weights of Japanese quails (Coturnix coturnix japonica). J Anim Vet Adv; 22:532-539.
• [4] Dekkers, J.C., & Hospital, F. (2002). The use of molecular genetics in the improvement of agricultural populations. Nat. Rev. Genet; 3:22-32.
• [5] Collard, B.C., Jahufer, M.Z., Brouwer, J.B., & Pang, E.C. (2005). An introduction to markers, quantitative trait loci (QTL) map-ping and marker-assisted selection for crop improvement: The basic concepts. Euphytica; 142:169-196.
• [6] Alboali, H., Moradi, M. H., Farahani, A. H. K., & Mohammadi, H. (2023). Genome-wide association study for body weight and feed consumption traits in Japanese quail using Bayesian approaches. Poult. Sci; 103208.
• [7] Fulton, J. E. (2012). Genomic selection for poultry breeding. Anim. Front; 2:30-36.
• [8] Kawahara-Miki, R., Sano, S., Nunome, M., Shimmura, T., Kuwayama, T., Takahashi, S., ... & Kono, T. (2013). Next-generation sequencing reveals genomic features in the Japanese quail. Genomics; 101:345-353.
• [9] Shi, Y., Chichung Lie, D., Taupin, P., Nakashima, K., Ray, J., Yu, R.T., ... & Evans, R.M. (2004). Expression and function of orphan nuclear receptor TLX in adult neural stem cells. Nature; 427:78-83.
• [10] Logan, C., Wingate, R. J., McKay, I. J., & Lumsden, A. (1998). Tlx-1 and Tlx-3 homeobox gene expression in cranial sensory ganglia and hindbrain of the chick embryo: markers of patterned connectivity. Journal of Neuroscience; 18: 5389-5402.
• [11] Yilmaz, A., Shen, S., Adelson, D. L., Xavier, S., & Zhu, J. J. (2005). Identification and sequence analysis of chicken Toll-like receptors. Immunogenetics; 56:743-753.
• [12] Fadhil, M., & Zülkadir, U. (2021). Association between polymorphisms of Myf5, MSTN and CAST genes and fattening per-formance in Brown Swiss and Holstein cattle breeds. Anim. Biotechnol; 32:121-129.
• [13] Ahmed, L. S., & Al-Barzinji, Y. M. S. (2020). Three candidate genes and its association with quantitative variation of egg pro-duction traites of local quail by using PCR-RFLP. Iraqi J. Agric. Sci; 51:124-131.
• [14] Sasazaki, S., Hinenoya, T., Lin, B., Fujiwara, A., & Mannen, H. (2006). A comparative map of macrochromosomes between chicken and Japanese quail based on orthologous genes. Anim. Genet; 37:316-320.
• [15] Sun, G., Alzayady, K., Stewart, R., Ye, P., Yang, S., Li, W., and Shi, Y. (2010). Histone demethylase LSD1 regulates neural stem cell proliferation. Mol. Cell. Biol; 30:1997-2005.
• [16] Zhang, C.L., Zou, Y., Yu, R.T., Gage, F.H., and Evans, R.M. (2006). Nuclear receptor TLX prevents retinal dystrophy and recruits the corepressor atrophin1. Genes Dev; 20:1308-1320.
• [17] Bozkaya, B., Aslan, E., and Patır, B. (2013). Determination of the Tal 1, Tal 2, SNAI 1 gene polymorphism of native Turkish chicken breeds by PCR-RFLP. Kafkas Univ Vet Fak Derg; 19:273-278.
• [18] Deef, E.E., and El-Nabi, S.E.A. (2015). Molecular screening of Nla III polymorphism in the chicken Tal-1 gene and its associa-tion with growth traits. Egypt. Poult. Sci. J; 35:977-989.