Determination of Monoamine Oxidase Inhibition and Antioxidant Effects of Various Herbal Teas

Yıl 2017, Cilt: 18 Sayı: 2, 105 - 113, 29.07.2017

Başak Yüce Dursun

https://doi.org/10.23902/trkjnat.302152

Cited By: 1

Öz

The
therapeutic properties of fruit teas are due to the polyphenolic flavonoids
they contain. Compounds inhibiting the flavoenzyme monoamine oxidase (EC
1.4.3.4, MAO) are known to be used as medicines in treatment of
neurodegenerative disorders such as Alzheimer’s and Parkinson’s diseases, and
in various neurological disorders. There are many studies about natural
compounds with antioxidant properties that prevent such diseases by reducing
oxidative stress which plays an important role in neurodegeneration. It is
therefore of particular importance to find double-acting products that can both
inhibit monoamine oxidase and reduce oxidative stress. In this study,
antioxidant activities of various fruit teas purchased from markets in Turkey
were determined by ferric-reducing antioxidant power, cupric reducing
antioxidant capacity and 1,1-diphenyl-2-picrylhydrazyl methods and their total
phenolic contents were revealed. MAO enzyme inhibitions of these tea infusions
was determined fluorometrically. The obtained results were compared with green
and black tea bag infusions. The results showed that antioxidant and MAO
inhibition properties of fruit tea samples were related to their total amount of
phenolic substances they contained. Pomegranate containing tea infusions had
higher antioxidant activity and phenolic content and exhibited high MAO
inhibition. The results also showed that a MAO–B selectivity varying between 2
to 3,5-fold existed in various tea infusions with the highest value in rose hip
tea. MAO inhibition abilities of tea infusions were demonstrated for the first
time in this present study which was based on the fact that phenolic substances
are responsible for MAO inhibition.

Anahtar Kelimeler

CUPRAC, tea infusion, DPPH, FRAP, MAO inhibition, Antioxidant activity, MAO inhibition

ÇEŞİTLİ BİTKİ ÇAYLARININ MONOAMİN OKSİDAZ İNHİBİSYONLARININ VE ANTİOKSİDAN ETKİLERİNİN BELİRLENMESİ

Öz

Meyve çaylarının terapötik özellikleri içerdikleri polifenolik
flavonoidlerden kaynaklanmaktadır. Bir flavoenzim olan monoamin oksidazı (EC
1.4.3.4, MAO) inhibe eden bileşiklerin, Alzheimer ve Parkinson gibi
nörodejeneratif rahatsızlıkların tedavisinde ve çeşitli nörolojik rahatsızlıklarda
ilaç olarak kullanıldığı bilinmektedir. Antioksidan özelliğe sahip doğal
bileşiklerin nörodejenerasyonda önemli bir rol oynayan oksidatif stresi
azaltarak bu hastalıkları önlediğine dair çok sayıda çalışma vardır. Bu nedenle
monoamin oksidaz enzimini inhibe edebilecek ve oksidatif stresi azaltabilecek
çift etkili ürünlerin bulunması ayrı bir önem taşımaktadır. Bu çalışmada Türkiye’deki
marketlerden satın alınan çeşitli meyve çaylarının; ferrik
indirgeyici antioksidan güç, bakır indirgeyici antioksidan kapasite ve 1,1-difenil-1-pikrilhidrazil radikal süpürücü metotları
ile antioksidan aktiviteleri saptanmıştır ve toplam fenolik madde miktarları
belirlenmiştir. Bu çay infüzyonlarının, MAO enzim inhibisyonları florometrik
olarak tayin edilmiştir. Elde edilen tüm sonuçlar poşet yeşil çay ve siyah çay
ile kıyaslanmıştır. Elde edilen sonuçlarda, bitki çayı örneklerinin antioksidan
ve MAO inhibisyon özelliklerinin içerdikleri toplam fenolik madde miktarı ile
ilişkili olduğu gösterilmiştir. Nar içeren çay infüzyonları daha yüksek
antioksidan aktivite ve fenolik madde içeriğine sahip olup aynı zamanda da yüksek
MAO inhibisyonu göstermektedir. Elde edilen sonuçlar, kuşburnu başta olmak
üzere çeşitli çay infüzyonlarında 2-3,5 kat arasında değişen MAO–B seçiciliği
bulunduğunu da göstermektedir. Fenolik maddelerin MAO inhibisyonuna sebep
olduğu bilgisinden yola çıkılarak yapılan bu çalışmada, çay infüzyonlarının MAO
inhibisyon yetenekleri de olduğu ilk kez ortaya konulmuştur.

Anahtar Kelimeler

CUPRAC, çay infüzyonu, DPPH, FRAP, MAO inhibisyonu, Antioksidan aktivite, MAO inhibisyonu

Kaynakça

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