GENETIC DIVERSITY IN SODIUM AZIDE INDUCED WHEAT MUTANTS STUDIED BY SSR MARKERS

Yıl 2018, Cilt: 19 Sayı: 2, 129 - 135, 15.10.2018

Ayşe Şen Fatma Sarsu

https://doi.org/10.23902/trkjnat.424305

Cited By: 1

Öz

Mutations
induced artificially way are one of the tools used to increase genetic
variation in populations where genetic variation has been shrinking especially
due to various reasons one of which is domestication. In this study, Simple
Sequence Repeats (SSRs) markers were used to screen genetic diversity in sodium
azide (NaN₃) induced fourteen fourth-generation advanced wheat
mutant lines. The mean values of polymorphism rate (29.44%), polymorphic
information content (PIC; 0.82), marker index (MI; 1.95) and resolving power
(Rp; 1.31) were calculated according to SSR marker profiles. Two SSRs, Xwmc170
and Xcfd6, were detected as the most polymorphic markers, Xgwm626 proved the highest PIC and MI values,
and Xcfd6 gave the highest Rp value. Unweighted
Pair Group Method with Arithmetic Mean (UPGMA) dendrogram classified 15 plants
into four groups. The Principle Component Analysis (PCA) showed 88.9% of the
total genetic variation. The results obtained in the present study might be
useful for determining the efficiency of NaN₃ for creating mutant
wheat lines with enough genetic variability to implement wheat-breeding
programs as germplasm resources.

Anahtar Kelimeler

Bread wheat, SSR markers, PCA, sodium azide mutagenesis, somatic embryogenesis, UPGMA

GENETIC DIVERSITY IN SODIUM AZIDE INDUCED WHEAT MUTANTS STUDIED BY SSR MARKERS

Öz

Yapay
yolla indüklenen mutasyonlar, genetik varyasyonun özellikle ıslah gibi çeşitli
nedenlerden dolayı küçüldüğü popülasyonlarda, çeşitliliği arttıran araçlardan
biridir. Bu çalışmada, sodyum azid (NaN₃) kullanılarak indüklenen on
dört dördüncü jenerasyon ileri mutant buğday hatlarında, genetik çeşitliliği
taramak için Basit Dizi Tekrarları (SSR) belirteçleri kullanıldı. SSR belirteç
profillerine göre ortalama polimorfizm oranı (% 29,44), polimorfik bilgi
içeriği (PIC; 0,82), belirteç indeksi (Mİ; 1,95) ve belirteç çözünürlük gücü
(Rp; 1,31) hesaplandı. İki SSR belirteci, Xwmc170 ve Xcfd6, en yüksek
polimorfizm oranına sahip belirteçler olarak tespit edildi. Xgwm626 en yüksek
PIC ve Mİ değerlerini, Xcfd6 de ​​en yüksek Rp değerini verdi. Ağırlıksız
Çift-Grup Yöntemi ile Aritmetik Ortalama (UPGMA) dendrogramı 15 bitkiyi dört
gruba ayırdı. Temel Bileşenler Analizi (PCA) toplam genetik varyasyonun %
88,9'unu gösterdi. Bu çalışma, buğday ıslah programlarında genetik kaynak
olarak kullanılmak üzere yeterli genetik çeşitliliğe sahip mutant buğday
hatlarını oluşturmak için sodyum azitin etkinliğinin gösterilmesi hususunda
yararlı olabilir.

Anahtar Kelimeler

Bread wheat, sodium azide mutagenesis, somatic embryogenesis, SSR markers, PCA, UPGMA

Kaynakça

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