Efficient Embryogenic Callus From Filaments With Anther In Thermopsis turcica

Dilek Tekdal; Selim Cetıner

Efficient Embryogenic Callus From Filaments With Anther In Thermopsis turcica

Yıl 2014, Cilt: 1 Sayı: Özel Sayı-1, 1242 - 1246, 01.03.2014

Öz

Thermopsis turcica is an endemic endangered species. The establishment of an improved micropropagation method is very important to preserve this species in vitro conditions. For this reason, the effects of different media and temperature pretreatments on the stamens of T. turcica planted at Nezahat Gokyigit Botanical Garden in Istanbul were investigated. T. turcica filaments with anther were excised and cultivated in vitro with Murashige and Skoog’s basal media supplemented with Naphthaleneacetic acid (4 mg L1), Benzylaminopurine (0.1 and 0.5 mg L-1), and AgNO3 (15 mg L-1) at 4, 28 and 37°C for a week under complete darkness. Moreover, to determine the effects of activated charcoal on regeneration, a medium containing 0.25% activated charcoal was also used. A week after culture initiation, all samples were then incubated at 28°C for a month under a 16 h photoperiod. The most promising callus formation was observed in filaments cultured on the media with 0.1 mg L-1 Benzylaminopurine (100%) pretreated at 37°C a week after culture initiation; however, the development of embryos from callus could also be visualized from the filaments cultured on the medium with 0.1 mg L-1 Benzylaminopurine (50%) pretreated at 4°C. None of the activated charcoal treatments yielded positive results regarding callus formation. Instead they negatively affected callus formation in the mixture media. Furthermore, control groups that were incubated on MS medium free from growth regulators did not show any growth. According to a literature review on T. turcica, no stamen culture has been performed to date

Anahtar Kelimeler

Callus, embryo, filament, Thermopsis turcica

Kaynakça

Arı, E., 2006. Anther Culture Studies on Anemone coronaria var. coccinea Native to Turkey. PhD Thesis, Cukurova University.
Cao, M.Q., Li, Y., Liu, F., Dore, C. (1994). Embryogenesis and plant regeneration of pakchoi (Brassica rapa L. ssp. chinensis) via in vitro isolated microspore culture. Plant Cell Reports, 13: 447-450.
Davis, P.H., Mill, R.R., Tan, K. (1988). Flora of Turkey and the East Aegean Islands, Edinburg University press, 10, 591 pp.
Ekim, T., Koyuncu, M., Vural, M., Duman, H., Aytaç, Z., Adiguzel, N. (2000). Red Data Book of Turkish Plants. Bariscan Ofset, Ankara, 246 pp.
Erik, S., Tarikahya, B. (2004). Flora of Turkey.
Kebikec, 17: 139-163.
Ferrie, A.M.R., Keller, W.A. (1995). Microspore culture for haploid plant production. Plant cell, tissue methods. Eds. Gamborg, O.L., Phillips, G.C., Springer, Berlin, pp. 155–164. Fundamental
Ferrie, A.M.R., Caswell, K.L. (2011). Isolated microspore culture techniques and recent progress for haploid and doubled haploid plant production. Plant Cell, Tissue and Organ Culture, 104: 301-309.
Kasha, K.J., Simion, E., Oro, R., Shim, Y.S. (2003). Barley isolated microspore culture protocol. Doubled haploid production in crop plants: a manual. Eds. Maluszynski, M., Kasha, K.J., Forster, B.P., Szarejko, I., Kluwer, Dordrecht, pp. 43–47.
Kim, M., Yang, I.C., Kim, J.A., Park, E.J., Yoon, M., Lee, Y. (2008). Embryogenesis and plant regeneration of hot pepper (Capsicum annuum L.) through isolated microspore culture. Plant Cell Reports, 27: 425-434.
Ochatt, S., Pech, C., Grewal, R., Conreux, C., Lulsdorf, M., Jacas, L. (2009). Abiotic stress enhances microspores some (Fabaceae). Journal of Plant Physiology, 166: 1314–1328. from legume species of
Ozdemir, C., Dural, H., Ertugrul, K., Kucukoduk, M., Baran, P., Sanda, M.A. (2008). Morphology and anatomy of endemic Thermopsis turcica Kit Tan, Vural & Kucukoduk. Bangladesh Journal of Botany, 37(2): 105-114.
Pechan, P.M., Bartels, D., Brown, D.C.W., Schell, J. (1991). Messenger-RNA and protein changes associated microspore embryogenesis, Planta, 184: 161– 165. of Brassica
Smykal, P. (2000). Pollen embryogenesis: the stress mediated switch sporophytic development, current status and future prospects, Biologia Plantarum, 43: 481– 489. gametophytic to
Stewart, J.N. (2008). Plant Biotechnology and Genetics: Applications. Wiley, New Jersey, 402 pp. Techniques, and
Tan, K., Vural, M., Kucukoduk, M. (1983). An unusual new Thermopsis from Turkey. Notes Royal Botanical Garden, Edinburgh, 40(3): 515- 518.
Tekdal, D., Cetiner, S. (2014). The determination of self-compability status of Thermopsis turcica through histological analysis. Journal of Applied Biological Sciences, 8(1): 64-67.
Vural, M. (2009). Piyan (Thermopsis turcica).
Bagbahce, 25: 14-16.