Investigation into Performance of Some Citrus Rootstocks in In vitro Drought Stress Conditions

Yıl 2018, Cilt: 28 Sayı: 3, 305 - 310, 28.09.2018

Özhan Şimşek Dicle Dönmez Yıldız Aka Kaçar

https://doi.org/10.29133/yyutbd.428140

Cited By: 8

Öz

Citrus is a fruit species
commercially in the tropical and subtropical regions of the world. Growth and
development of plants are affected by environmental factors such as salinity
and drought. Global climate change will increase water stress risk in the near
future.  One of the purposes of plant
biotechnology is the development of plants tolerant to drought. Among
environmental stresses, drought stress is one of the factors that negatively
affect plant growth and yield. The response of plants to drought stress is
quite complex and is the process of many genetic expressions. In this study,
Troyer citrange and C-35 citrange from the significant citrus rootstocks were
used. After seeds of plant materials were germinated drought stress was applied
in in vitro condition. The response
of the plants to drought and performance of micropropagation has been
determined. It was determined that both rootstocks continued their life and
proliferation in increasing PEG doses, however their performance found to be
declining.

Anahtar Kelimeler

Abiotic stress, Plant tissue culture, C-35, PEG, Troyer citrange

Bazı Turunçgil Anaçlarının In vitro Kuraklık Stresi Koşullarında Performanslarının Araştırılması

Öz

Turunçgiller dünyanın tropik ve subtropik
bölgelerinde ticari olarak yetiştiriciliği yapılan bir meyve türüdür.  Bitkilerin büyüme ve gelişimleri tuzluluk,
kuraklık gibi abiyotik faktörlerden etkilenmektedir.  Küresel iklim değişikliğinin yakın gelecekte
su stresi riskini artıracağı beklenmektedir. 
Bitki biyoteknolojisinin amaçlarından biri kuraklığa tolerant bitkilerin
geliştirilmesidir.  Çevresel stresler
arasında kuraklık stresi bitki büyüme ve verimini en olumsuz etkileyen
faktörlerden biridir.  Bitkilerin
kuraklık stresine verdiği cevap oldukça karmaşık ve birçok genin ifadesinin
gerçekleştiği bir süreçtir.  Bu çalışmada
turunçgil anaçları arasında yer alan Troyer sitranjı ve C-35 sitranjı
kullanılmıştır.  Bitkisel materyallere
ait tohumlar çimlendirildikten sonra in
vitro koşullarda kuraklık stresi uygulanmıştır.  Bitkilerin in vitro’da kuraklık stresi altında çoğaltım performansları ve
verdikleri tepkiler belirlenmiştir.  Her
iki anacında artan PEG dozlarında yaşamları ve çoğalmalarına devam ettirdikleri
ancak performanslarının gerilediği tespit edilmiştir.  

Anahtar Kelimeler

PEG, Abiyotik stres, Bitki doku kültürü, C-35, Troyer sitranjı

Kaynakça

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