Molecular Epidemiology and Risk Factors Assessment of Anaplasma spp. on Dairy Cattle in Southwest of Iran

Yıl 2019, Cilt: 45 Sayı: 1, 30 - 36, 01.01.2019

Vahid Noaman Morteza Moradı

Öz

DOI:
10.26650/actavet.2019.18014

The present study was carried out to
determine Anaplasma species and potential risk factors associated with
molecular prevalence of Anaplasma spp. among dairy cattle in southwest of Iran.
A total of 88 samples out of 200 generated an expected amplicon of 866 bp from
Anaplasma marginale msp4 gene. Six samples that were identified as A. marginale
gave also positive results for A. phagocytophilum 16S rRNA gene with specific
nested polymerase chain reaction (nPCR). The multivariate analysis of risk
factors revealed that the cattle of mountain regions were significantly
(p=0.0001) at higher risk as compared to the plain regions. Cattle <1 year
age and the latitude 32-33°C were significantly at lower risk (p<0.01). The
cattle with low milk yield were significantly (p=0.002) at lower risk. Low
hygienic farms were significantly (p=0.011) at higher risk as compared to good
and normal hygienic farms. Distance from other farms (<1Km) was another
important risk factor which showed significant association with the occurrence
of Anaplasma infection (p=0.021). The results of this study can be used in
strategic planning for prevention and control of bovine anaplasmosis in dairy
cattle in the southwest of Iran.

Anahtar Kelimeler

Anaplasma, cattle, Iran, molecular epidemiology, risk factors

Kaynakça

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