Year 2018, Volume 2, Issue 3, Pages 186 - 218 2018-11-12

The correlation with prognostic factors of bcl-2 and cathepsin D in invasive ductal carcinomas
İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi

MUSTAFA KÖSEM [1] , İBRAHİM GELİNCİK [2] , İRFAN BAYRAM [3] , MEHMET ÇETİN KOTAN [4] , AYHAN YILDIRIM [5]

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Introduction: Invasive ductal carcinoma which is cancers of the breast is the most common cancer of the breast in women. In this tumors, early diagnosis and appropriate medical treatment is to influence markedly to life span. For this reason, cancers of the breast is occured intesify at therapy with chemotherapotic agents and hormonal treatment for the purpose of prevention systemic spread and prognosis determination of works in the last years. Just reliable determinatives are necessary, because of not to be adequate to orientate treatment and prognosis only classıc parameters. For this reason, it was evaluated the correlation with each other and other prognostic factors of bcl-2 and cathepsin D in invasive ductal carcinomas of the breast in the present study.

Materials and methods: In the present study. it was included 50 cases diagnosed with invasive ductal carcinoma and examined mastectomy and axillary dissection material. Slides stained  with estrogen, progesterone and c-erbB-2 were reviewed again and done scoring separately for each slide.Also in the present study it was stained bcl-2 and cathepsin D expression in 50 invasive ductal carcinomas by immünhistochemistry, and evaluated the correlation with other prognostic factors and each other.

Results: A strong positive relationship was not detected between bcl-2 expression with other prognostic factors and cathepsin D protein, while a strong positive relationship was detected between bcl-2 expression with low nuclear grade and progesterone receptor’ status. A strong correlation was not observed between cathepsin D expression with oestrogen receptor’ status and progesterone receptor’ status and c-erbB-2 oncogene amplification and other prognostic factors, while a strong correlation was also observed between cathepsin D expression with high nuclear grade and blood vessel invasion and lymph-vessel invasion and perineural invasion.

Conclusion: Bcl-2 is thought to be important in determining of invasive ductal carcinoma cases with low grade, while cathepsin D is thought to be important in determining of invasive ductal carcinoma cases with high grade and invasion ability. 

GİRİŞ

Meme kanserlerinden invaziv duktal karsinom, kadınlarda en sık görülen meme kanseridir. Bu tümörlerde erken tanı ve uygun tedavi yaşam süresini önemli ölçüde etkilemektedir. Bu nedenle son yıllardaki çalışmaların, meme kanserlerinde prognozu belirleme ve sistemik yayılımı önleme amacıyla hormonal ve kemoterapötik ajanlarla tedavide yoğunlaştığı görülmektedir. Sadece klasik parametrelerin prognozu ve tedaviyi yönlendirmede yeterli olmaması nedeniyle yeni, güvenilir belirleyicilere gerek vardır. Bu nedenle bu çalışmada, memenin invaziv duktal karsinom (İDK)’unda bcl-2 ve katepsin D’nin birbirleriyle ve diğer prognostik faktörlerle olan ilişkisi araştırıldı.

GEREÇ VE YÖNTEM

2004-2006 yılları arasında Yüzüncü Yıl Üniversitesi Tıp Fakültesi Patoloji Ana Bilim Dalında mastektomi ve aksiller diseksiyon materyali incelenen ve invaziv duktal karsinom tanısı alan 50 adet olgu çalışmaya alındı. Bu olguların tümör diferansiyasyonu, histolojik derece ve nükleer skor (NS)’ları ile mitoz sayısı Bloom Richardson'a göre ayrı ayrı değerlendirildi. Bunun yanısıra tümör çevresi lenfatik, kan damarı ve perinöral invazyonun varlığı araştırıldı. Tümörün deri ile ilişkisi saptandı. Tümör çapı ve aksiller lenf nodlarının durumu değerlendirildi. Multifokal ve multisentrik tümörlerde, en büyük tümörün çapı ele alındı. Östrojen, progesteron ve c-erbB-2 ile boyanmış olan lamlar tekrar gözden geçirilerek her biri için ayrı ayrı skorlama yapıldı.

Ayrıca 50 invaziv duktal karsinom olgusu, bcl-2 ve katepsin D ile immünhistokimyasal olarak boyandı ve her biri için ayrı ayrı skorlama yapılarak sonuçlar birbirleri ve diğer prognostik faktörlerle karşılaştırılarak değerlendirildi.

BULGULAR

Progesteron reseptörü ve düşük NS ile bcl-2 ekspresyonu arasında güçlü pozitif bir ilişki tespit edilirken katepsin D ve diğer prognostik faktörlerle bcl-2 ekspresyonu arasında güçlü pozitif bir ilişki tespit edilmedi. Perinöral invazyon, lenf damarı invazyonu, kan damarı invazyonu ve yüksek NS ile katepsin D ekspresyonu arasında güçlü pozitif bir ilişki gözlenirken diğer prognostik faktörler, c-erbB2, progesteron reseptörü, östrojen reseptörü ile katepsin D ekspresyonu arasında güçlü bir ilişki gözlenmedi.

SONUÇ

Bcl-2’nin düşük dereceli, katepsin D’nin ise yüksek dereceli ve invazyon yeteneği kazanmış invaziv duktal karsinom olgularının belirlenmesinde önemli olabileceği düşünülmektedir.

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Primary Language tr
Subjects Medicine
Journal Section MAKALELER
Authors

Author: MUSTAFA KÖSEM
Institution: Sakarya Üniversitesi Tıp Fakültesi Hastanesi, Patoloji A.D./ Sakarya
Country: Turkey


Author: İBRAHİM GELİNCİK (Primary Author)
Institution: Namık Kemal Üniversitesi Tıp Fakültesi Hastanesi, Patoloji A.D./ Tekirdağ
Country: Turkey


Author: İRFAN BAYRAM
Institution: Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Patoloji A.D./ Van
Country: Turkey


Author: MEHMET ÇETİN KOTAN
Institution: Yüzüncü Yıl Üniversitesi Tıp Fakültesi, Genel Cerrahi A.D./ Van
Country: Turkey


Author: AYHAN YILDIRIM
Institution: Diyarbakır Kadın Doğum Ve Çocuk Hastalıkları Hastanesi, Patoloji Bölümü/ Diyarbakır
Country: Turkey


Dates

Publication Date: November 12, 2018

Bibtex @research article { bmedj458146, journal = {Balıkesir Medical Journal}, issn = {}, eissn = {2564-6664}, address = {Balıkesir University}, year = {2018}, volume = {2}, pages = {186 - 218}, doi = {}, title = {İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi}, key = {cite}, author = {KÖSEM, MUSTAFA and GELİNCİK, İBRAHİM and BAYRAM, İRFAN and KOTAN, MEHMET ÇETİN and YILDIRIM, AYHAN} }
APA KÖSEM, M , GELİNCİK, İ , BAYRAM, İ , KOTAN, M , YILDIRIM, A . (2018). İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi. Balıkesir Medical Journal, 2 (3), 186-218. Retrieved from http://dergipark.org.tr/bmedj/issue/40326/458146
MLA KÖSEM, M , GELİNCİK, İ , BAYRAM, İ , KOTAN, M , YILDIRIM, A . "İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi". Balıkesir Medical Journal 2 (2018): 186-218 <http://dergipark.org.tr/bmedj/issue/40326/458146>
Chicago KÖSEM, M , GELİNCİK, İ , BAYRAM, İ , KOTAN, M , YILDIRIM, A . "İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi". Balıkesir Medical Journal 2 (2018): 186-218
RIS TY - JOUR T1 - İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi AU - MUSTAFA KÖSEM , İBRAHİM GELİNCİK , İRFAN BAYRAM , MEHMET ÇETİN KOTAN , AYHAN YILDIRIM Y1 - 2018 PY - 2018 N1 - DO - T2 - Balıkesir Medical Journal JF - Journal JO - JOR SP - 186 EP - 218 VL - 2 IS - 3 SN - -2564-6664 M3 - UR - Y2 - 2018 ER -
EndNote %0 Balıkesir Medical Journal İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi %A MUSTAFA KÖSEM , İBRAHİM GELİNCİK , İRFAN BAYRAM , MEHMET ÇETİN KOTAN , AYHAN YILDIRIM %T İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi %D 2018 %J Balıkesir Medical Journal %P -2564-6664 %V 2 %N 3 %R %U
ISNAD KÖSEM, MUSTAFA , GELİNCİK, İBRAHİM , BAYRAM, İRFAN , KOTAN, MEHMET ÇETİN , YILDIRIM, AYHAN . "İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi". Balıkesir Medical Journal 2 / 3 (November 2018): 186-218.
AMA KÖSEM M , GELİNCİK İ , BAYRAM İ , KOTAN M , YILDIRIM A . İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi. Balıkesir Medical Journal. 2018; 2(3): 186-218.
Vancouver KÖSEM M , GELİNCİK İ , BAYRAM İ , KOTAN M , YILDIRIM A . İnvaziv duktal karsinomlarda bcl 2 ve katepsin D’nin prognostik faktörlerle ilişkisi. Balıkesir Medical Journal. 2018; 2(3): 218-186.