Virulence of Entomopathogenic Fungi and Bacteria against Stored Product Pests

Yıl 2015, Cilt: 11 Sayı: 2, 79 - 87, 18.12.2015

Ali Sevim Elif Sevim Meryem Demirci

https://doi.org/10.18466/cbujos.71031

Cited By: 3

Öz

Virulence of Entomopathogenic Fungi and Bacteria against Stored Product Pests Entomopathogenic microorganisms such as bacteria, fungi, viruses, nematodes and protozoa play an important role for regulation of insect pest populations and, this leads to use these microorganisms as biological control agents against pest species as an alternative to chemicals insecticides. In this study, we tested different bacteria originated from stored product pests and fungi isolated from different sources against two important stored product pests, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae) and Callosobruchus maculatus (F) (Coleoptera: Bruchidae), under laboratory conditions. Based on the laboratory screening, the highest mortality against A. obtectus within bacteria and fungi was obtained from Staphylococcus kloosii Fbe-10 with 73% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Lecanicillium muscarium ARSEF3600 and Beauveria bassiana ARSEF8668 with 100%. The highest mortality against C. maculatus within bacteria and fungi was obtained from Bacillus pumilus Be-2with 57% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Beauveria bassiana ARSEF8668 with 90%. These results showed that the fungal isolates used in this study seem to be more effective than bacteria and, should be further investigated in terms of developing microbial control agent against stored product pestsVirulence of Entomopathogenic Fungi and Bacteria against Stored Product Pests Entomopathogenic microorganisms such as bacteria, fungi, viruses, nematodes and protozoa play an important role for regulation of insect pest populations and, this leads to use these microorganisms as biological control agents against pest species as an alternative to chemicals insecticides. In this study, we tested different bacteria originated from stored product pests and fungi isolated from different sources against two important stored product pests, Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae) and Callosobruchus maculatus (F) (Coleoptera: Bruchidae), under laboratory conditions. Based on the laboratory screening, the highest mortality against A. obtectus within bacteria and fungi was obtained from Staphylococcus kloosii Fbe-10 with 73% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Lecanicillium muscarium ARSEF3600 and Beauveria bassiana ARSEF8668 with 100%. The highest mortality against C. maculatus within bacteria and fungi was obtained from Bacillus pumilus Be-2with 57% and Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 and, Beauveria bassiana ARSEF8668 with 100%, respectively. Also, the highest mycosis within fungi was obtained from Beauveria bassiana ARSEF8668 with 90%. These results showed that the fungal isolates used in this study seem to be more effective than bacteria and, should be further investigated in terms of developing microbial control agent against stored product pests

Anahtar Kelimeler

Bacteria, fungi, microbial control, stored product pests

Depolanmış Ürün Zararlılarına Karşı Entomopatojenik Fungus ve Bakterilerin Virulansları

Öz

Bakteri, fungus, virus, nematod ve protozoa gibi entomopatojenik mikroorganizmalar zararlı böcek popülasyonlarının düzenlenmesinde önemli rol oynamaktadırlar ve bu entomopatojen mikroorganizmaların kimyasal insektisidlere alternative olarak zararlı böceklere karşı biyolojik mücadele etmeni olarak kullanımına yol açmaktadır. Bu çalışmada, depolanmış ürün zararlılarından izole edilen farklı bakteriler ve farklı kaynaklardan izole edilen funguslar iki önemli depolanmış ürün zararlısı olan Acanthoscelides obtectus (Say) (Coleoptera: Chrysomelidae) veCallosobruchus maculatus (F) (Coleoptera: Bruchidae)’a karşı laboratuar koşulları altında test edilmiştir. Laboratuar tarama testlerine göre, A. obtectus’a karşı en yüksek ölüm %73 ile bakteriler arasından Staphylococcus kloosii Fbe-10’dan ve %100 ile funguslar arasındanLecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 veBeauveria bassiana ARSEF8668’den elde edilmiştir. Funguslar arasından en yüksek mikozlanma oranı ise %100 ile Lecanicillium muscarium ARSEF3600 ve Beauveria bassiana ARSEF8668’den elde edilmiştir. C. maculatus’a karşı ise en yüksek ölüm %57 ile bakteriler arasından Bacillus pumilus Be-2’den ve %100 ile funguslar arasından Lecanicillium muscarium ARSEF3600, Beauveria pseudobassiana ARSEF8664 veBeauveria bassiana ARSEF8668’den elde edilmiştir. Funguslar arasında en yüksek mikozlanma oranı ise %90 ile Beauveria bassiana ARSEF8668’den elde edilmiştir. Bu sonuçlar bu çalışmada kullanılan fungal izolatların bakterilerden daha etkili olduğunu ve bu bakterilerin depolanmış ürün zararlılarına karşı mikrobiyal mücadele etmeni olarak geliştirilmesi amacıyla daha fazla araştırılması gerektiğini göstermektedir.

Anahtar Kelimeler

Bakteri, fungus, mikrobiyal mücadele, depolanmış ürün zararlıları

Kaynakça

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