Year 2019, Volume 15, Issue 2, Pages 205 - 210 2019-06-30

The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots

Rengin Özgür Uzilday [1]

10 17

Magnesium (Mg) is an essential nutrient that takes vital roles in biochemical processes related to energy metabolism, regulation of enzyme activity, nucleic acid biosynthesis and carbohydrate metabolism. Mg deficiency in arable lands is an emerging problem. Loss of balance in different metabolic pathways due to Mg deficiency causes increased production of reactive oxygen species (ROS) such as O2.-, H2O2 and HO. in the roots.  The aim of this work was to elucidate the antioxidant response of roots of cotton (Gossypium hirsutum) to Mg deficiency. For this aim, antioxidant enzyme activities were measured and changes in their isoenzymes were determined. In addition, NADPH oxidase (NOX) activity was investigated to determine the changes in ROS signaling. Moreover, H2O2 and lipid peroxidation levels were also measured as an indicator of oxidative damage. Findings of this work indicate that Mg deficiency does not cause oxidative load in roots of Gossypium hirsutum plants that overwhelms cellular antioxidant defence system, as evident by H2O2 and TBARS levels. However, to adjust to the new redox status, root cells drastically downregulate NOX dependent ROS production and induced total superoxide dismutase activity. These findings indicate that site of O2.- production is mitochondria in roots. Although, catalase and ascorbate peroxidase activities were decreased with Mg deficiency induced peroxidase activity seems to limit H2O2 accumulation. These adaptive responses and changes in antioxidant defence were sufficient to prevent excess ROS accumulation and occurrence of oxidative damage in roots of Gossypium hirsutum plants under Mg deficiency
antioxidant enzymes, cotton, Gossypium hirsutum, magnesium deficiency, reactive oxygen species, Antioxidant defense system, reactive oxygen species
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Primary Language en
Subjects Engineering
Published Date 30-06-2019
Journal Section Articles
Authors

Orcid: 0000-0002-4312-8093
Author: Rengin Özgür Uzilday (Primary Author)
Institution: EGE UNIVERSITY
Country: Turkey


Dates

Publication Date: June 30, 2019

Bibtex @research article { cbayarfbe523259, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar University}, year = {2019}, volume = {15}, pages = {205 - 210}, doi = {10.18466/cbayarfbe.523259}, title = {The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots}, key = {cite}, author = {Özgür Uzilday, Rengin} }
APA Özgür Uzilday, R . (2019). The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots. Celal Bayar University Journal of Science, 15 (2), 205-210. DOI: 10.18466/cbayarfbe.523259
MLA Özgür Uzilday, R . "The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots". Celal Bayar University Journal of Science 15 (2019): 205-210 <http://dergipark.org.tr/cbayarfbe/issue/46535/523259>
Chicago Özgür Uzilday, R . "The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots". Celal Bayar University Journal of Science 15 (2019): 205-210
RIS TY - JOUR T1 - The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots AU - Rengin Özgür Uzilday Y1 - 2019 PY - 2019 N1 - doi: 10.18466/cbayarfbe.523259 DO - 10.18466/cbayarfbe.523259 T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 205 EP - 210 VL - 15 IS - 2 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.523259 UR - https://doi.org/10.18466/cbayarfbe.523259 Y2 - 2019 ER -
EndNote %0 Celal Bayar University Journal of Science The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots %A Rengin Özgür Uzilday %T The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots %D 2019 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 15 %N 2 %R doi: 10.18466/cbayarfbe.523259 %U 10.18466/cbayarfbe.523259
ISNAD Özgür Uzilday, Rengin . "The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots". Celal Bayar University Journal of Science 15 / 2 (June 2019): 205-210. https://doi.org/10.18466/cbayarfbe.523259
AMA Özgür Uzilday R . The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots. Celal Bayar Univ J Sci. 2019; 15(2): 205-210.
Vancouver Özgür Uzilday R . The Effects of Mg Deficiency on Activities and Isoenzyme Patterns of Antioxidant Enzymes in Cotton Roots. Celal Bayar University Journal of Science. 2019; 15(2): 210-205.