Year 2019, Volume 15, Issue 2, Pages 221 - 226 2019-06-30

Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam

Recep Liman [1] , Seçil Özkan [2]

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Penoxsulam is a new sulfonamide herbicide used on rice crops for annual grasses, broadleaf weeds, and sedges control, either grown through transplanting or direct dry or direct seeding methods of planting. In this study, Allium cepa ana-telophase and comet assay were used to examine the cyto-genotoxic effects of herbicide penoxsulam on A. cepa roots. A. cepa bulbs were exposed to ½xEC50 (12.5 µg/L), EC50 (25 µg/L) and 2xEC50 (50 µg/L) concentrations of penoxsulam for 24, 48, 72 and 96 h. Distilled water and 10 ppm of methyl methanesulfonate (MMS) were used as negative and positive control groups, respectively. Penoxsulam showed a cytotoxic effect by reducing root growth and mitotic index (MI), a genotoxic effect because it statistically increased chromosome aberrations (CAs, anaphase bridge, chromosomal laggards, polyploidy, disturbed ana-telophase and stickiness) and DNA damage as compared to control. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was also used for quantitative analysis of penoxsulam in A. cepa root meristem cells. Further molecular toxicological evaluations associated in the cyto-genotoxicity of penoxsulam on plants are needed to confirm these results
Allium cepa Ana-Telophase Test, Chromosome Aberrations, Comet Assay, Mass Spectrometry, Penoxsulam, Herbicide
  • 1. Fujiwara, TO, Hagan, D. 2014. Successful fluorine-containing herbicide agrochemicals. Journal of Fluorine Chemistry; 167: 16-29.
  • 2. Koschnick, TJ, Netherland, MD, Haller, WT. 2007. Effects of three als-inhibitors on five emergent native plant species in Florida. Journal of Aquatic Plant Management; 45: 47–51.
  • 3. Jabusch, TW, Tjeerdema, RS. 2005. Partitioning of penoxsulam, a new sulfonamide herbicide. Journal of Agrıcultural and Food Chemistry; 53(18): 7179-7183.
  • 4. Patetsini, E, Dimitriadis, VK, Kaloyianni, M. 2013. Biomarkers in marine mussels, Mytilus galloprovincialis, exposed to environmentally relevant levels of the pesticides, chlorpyrifos and penoxsulam. Aquatic Toxicology; 126: 338-345.
  • 5. Leme, DM, Marin-Morales, MA. 2009. Allium cepa test in environmental monitoring: a review on its application. Mutation Research-Reviews in Mutation Research; 682(1): 71-81.
  • 6. Liman, R, Cigerci, IH, Akyil, D, Eren, Y. 2011. Konuk M. Determination of genotoxicity of fenaminosulf by allium and comet tests. Pesticide Biochemistry and Physiology; 99, 61–64. 7. Fiskesjö, G. 1988. The allium test-an alternative in environmental studies: the relative toxicity of metal ions. Mutation Research; 197: 243–260.
  • 8. Rank, J, Nielsen, MH. 1994. Evaluation of the allium anaphase-telophase test in relation to genotoxicity screening of industrial wastewater. Mutation Research-Environmental Mutagenesis and Related Subjects; 312(1): 17-24.
  • 9. Saxena, PN, Chauhan, LKS, Gupta, SK. 2005. Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: spectroscopic basis of chromosome damage. Toxicology; 216(2-3): 244-252.
  • 10. Tice, RR, Agurell, E, Anderson, D, Burlinson, B, Hartman, A, Kobayashi, H, Sasaki, YF. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environmental and Molecular Mutagenesis; 35(3): 206-221.
  • 11. Koçyiğit, A, Keles, H, Selek, S, Guzel, S, Celik, H, Erel, O. 2005. Increased DNA damage and oxidative stress in patients with cutaneous leishmaniasis. Mutation Research; 585: 71–78.
  • 12. Concenço, G, Lopes, NF, Moraes, DM, Andres, A, Melo, PTBS. 2006. Rice seedling and plant development as affected by increasing rates of penoxsulam under controlled environments. Planta Daninha; 24(1): 131-139.
  • 13. Raj, SK, Syriac, EK, Geetha, D. 2018. Non-target effect of herbicide mixtures on the mycelial growth of Rhizoctonia solani kuhn. Japanese Journal of Tropical Agriculture; 55(2): 214-219.
  • 14. Silveira, MAD, Ribeiro DL, De Castro Marcondes, JP, D’arce, LPG. 2016. Sulfentrazone and flumetsulam herbicides caused DNA damage and instability in Allium cepa test. International Journal of Education and Applied Research; 2(8): 1-7.
  • 15. Soliman, MI, Ghoneam, GT. 2004. The mutagenic potentialities of some herbicides using Vicia faba as a biological system. Biotechnology Journal; 3: 140–154.
  • 16. Rangaswamy, V, Shanthamurthy, KB, Arekal, GD. 1979. Cytological effects of industrial effluent on somatic cells of Allium cepa. Cytologia; 44: 921-926.
  • 17. Billington, R, Gehen, SC, Hanley, TR. Toxicology of Triazolopyrimidine Herbicides. In: Robert K (ed) Hayes' Handbook of Pesticide Toxicolog, 3rd edn. Academic Press, United States, 2010, pp 1865-1885.
  • 18. Costa, R, Pereira, JL, Santos, MA, Pacheco, M, Guilherme, S. 2018. The role of contamination history and gender on the genotoxic responses of the crayfish Procambarus clarkii to a penoxsulam-based herbicide. Ecotoxicology; 27(7): 908-918.
  • 19. Nohatto, MA, Agostinetto, D, Langaro, AC, Oliveira, CD, Ruchel, Q. 2016. Antioxidant activity of rice plants sprayed with herbicides. Pesquisa Agropecuária Tropical; 46(1): 28-34.
  • 20. Galal, AA, Reda, RM, Mohamed, AAR. 2018. Influences of Chlorella vulgaris dietary supplementation on growth performance, hematology, immune response and disease resistance in Oreochromis niloticus exposed to sub-lethal concentrations of penoxsulam herbicide. Fish and Shellfish Immunology; 77: 445-456.
Primary Language en
Subjects Engineering
Published Date 30-06-2019
Journal Section Articles
Authors

Author: Recep Liman (Primary Author)
Institution: USAK UNIVERSITY, FACULTY OF SCIENCE AND LETTERS
Country: Turkey


Author: Seçil Özkan
Institution: USAK UNIVERSITY, INSTITUTE OF SCIENCE
Country: Turkey


Dates

Publication Date: June 30, 2019

Bibtex @research article { cbayarfbe533466, journal = {Celal Bayar University Journal of Science}, issn = {1305-130X}, eissn = {1305-1385}, address = {Celal Bayar University}, year = {2019}, volume = {15}, pages = {221 - 226}, doi = {10.18466/cbayarfbe.533466}, title = {Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam}, key = {cite}, author = {Liman, Recep and Özkan, Seçil} }
APA Liman, R , Özkan, S . (2019). Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam. Celal Bayar University Journal of Science, 15 (2), 221-226. DOI: 10.18466/cbayarfbe.533466
MLA Liman, R , Özkan, S . "Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam". Celal Bayar University Journal of Science 15 (2019): 221-226 <http://dergipark.org.tr/cbayarfbe/issue/46535/533466>
Chicago Liman, R , Özkan, S . "Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam". Celal Bayar University Journal of Science 15 (2019): 221-226
RIS TY - JOUR T1 - Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam AU - Recep Liman , Seçil Özkan Y1 - 2019 PY - 2019 N1 - doi: 10.18466/cbayarfbe.533466 DO - 10.18466/cbayarfbe.533466 T2 - Celal Bayar University Journal of Science JF - Journal JO - JOR SP - 221 EP - 226 VL - 15 IS - 2 SN - 1305-130X-1305-1385 M3 - doi: 10.18466/cbayarfbe.533466 UR - https://doi.org/10.18466/cbayarfbe.533466 Y2 - 2019 ER -
EndNote %0 Celal Bayar University Journal of Science Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam %A Recep Liman , Seçil Özkan %T Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam %D 2019 %J Celal Bayar University Journal of Science %P 1305-130X-1305-1385 %V 15 %N 2 %R doi: 10.18466/cbayarfbe.533466 %U 10.18466/cbayarfbe.533466
ISNAD Liman, Recep , Özkan, Seçil . "Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam". Celal Bayar University Journal of Science 15 / 2 (June 2019): 221-226. https://doi.org/10.18466/cbayarfbe.533466
AMA Liman R , Özkan S . Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam. Celal Bayar Univ J Sci. 2019; 15(2): 221-226.
Vancouver Liman R , Özkan S . Cytotoxicity and Genotoxicity in Allium cepa L. Root Meristem Cells Exposed to the Herbicide Penoxsulam. Celal Bayar University Journal of Science. 2019; 15(2): 226-221.