Year 2019, Volume 6, Issue 2, Pages 74 - 80 2019-06-28

Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması
Investigation of Cytotoxic Effect of Origanum minutiflorum on Cancer Cells

OKTAY ÖZKAN [1] , Caner Özbey [2] , Işıl Aydemir [3] , İsmail Sari [4] , Gonca Dönmez [5] , Fatma Esin Kırık [6] , Ahmet Savran [7] , Mehmet İbrahim Tuğlu [8]

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Amaç: Kanser, doku ve organları istila eden ve vücudun diğer kısımlarına sıçrayabilen, hücrelerin anormal gelişimidir. Meme kanseri en çok tekrarlanan tanıya sahip ve dünya genelinde kadınlarda kanserle ilişkili ölümlerde en önde gelen sebeplerdendir. Tıbbi bitkiler bazı kanser türlerinde antikarsinojenik özelliklere sahip olabilmektedir. Bu çalışmanın amacı Origanum minutiflorum O. Schwarz & P.H. Davis’in in vitro şartlarda, meme kanseri hücre dizilerinde sitotoksik etkisini araştırmaktır.

Gereç ve Yöntem: Bu çalışmada, MDA-MB-231 ve MCF-7 meme kanseri hücre dizileri ve adipoz dokudan izole edilmiş mezenkimal kök hücreleri kullanıldı. Origanum minutiflorum’dan elde edilmiş esansiyel yağa maruz bırakılmış tüm hücre dizilerinde MTT testi aracılığıyla IC50 dozu hesaplandı. Her bir hücre dizisinin IC50 dozunda  eNOS, p53, PCNA ve TUNEL için immunositokimya boyaması yapıldı. Sonuçlar Graphpad yazılımı kullanılarak one-way-ANOVA kullanılarak değerlendirildi.

Bulgular: Meme kanseri hücrelerinin proliferasyonu oragano yağı tarafından inhibe edildi. Oregano yağı mezenkimal kök hücrelerinde herhangi bir sitotoksik etki göstermedi. eNOS ve p53 ile apoptozisin göstergesi TUNEL’de artış yoluyla oreagono yağının kanser hücrelerinde sitotoksik olduğu ortaya çıktı ve PCNA boyamada proliferasyonun azaldığı tespit edildi. Sonuç: Bizim verilerimize göre oregano yağı MDA-MB-231 ve MCF-7 meme kanseri hücrelerinde sitotoksik bir etkiye sahiptir ve diğer biyolojik aktiviteleri için aktif komponentlerini tanımlayacak testlere ihtiyaç vardır. 

Objective: Cancer is defined by a abnormal growth of cells, that invade tissues and organs and spread to other parts of the body. Breast cancer is one of the most repeatedly diagnosed cancers and the foremost cause of death among women worldwide. Medicinal plants can have an anticancerogenic effect in some cancer types. Our aim is to search the cytotoxic effect of Origanum minutiflorum O. Schwarz & P.H. Davis on breast cancer cells in vitro conditions.

Methods: In this study, MDA-MB-231 and MCF-7 breast cancer cell lines and adipose tissue derived mesenchymal stem cells were used. Essential oil from Origanum minutiflorum was exposed to the all cells and IC50 dose was calculated by MTT assay. We have examined immunocytochemical staining of eNOS, p53, PCNA and TUNEL at IC50 dose for each cells. The results were evaluated using one-way-ANOVA by Graphpad software.

Results: The proliferation of breast cancer cells were inhibited by oregano oil. Oregano oil did not show any cytotoxic effect in mesenchymal stem cells. It was determined that cytotoxic effect of oregano oil on cancer cells was occurred through increase of eNOS and p53 staining for survival and the TUNEL labeling for apoptosis, and decreased of PCNA staining for proliferation.

Conclusion: According to our data, oregano oil has cytotoxic effect on MDA-MB-231 and MCF-7 breast cancer cell lines, and it needs to be analysis to detect active components for other biological activities.

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Primary Language tr
Subjects Medicine
Journal Section Araştırma Makalesi
Authors

Orcid: 0000-0002-9794-8863
Author: OKTAY ÖZKAN (Primary Author)
Institution: Niğde Üniversitesi Tıp Fakültesi
Country: Turkey


Author: Caner Özbey
Country: Turkey


Author: Işıl Aydemir
Country: Turkey


Author: İsmail Sari
Country: Turkey


Author: Gonca Dönmez
Country: Turkey


Author: Fatma Esin Kırık
Country: Turkey


Author: Ahmet Savran
Country: Turkey


Author: Mehmet İbrahim Tuğlu
Country: Turkey


Dates

Publication Date: June 28, 2019

Bibtex @research article { cbusbed405472, journal = {Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi}, issn = {}, eissn = {2147-9607}, address = {Celal Bayar University}, year = {2019}, volume = {6}, pages = {74 - 80}, doi = {10.34087/cbusbed.405472}, title = {Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması}, key = {cite}, author = {ÖZKAN, OKTAY and Özbey, Caner and Aydemir, Işıl and Sari, İsmail and Dönmez, Gonca and Kırık, Fatma Esin and Savran, Ahmet and Tuğlu, Mehmet İbrahim} }
APA ÖZKAN, O , Özbey, C , Aydemir, I , Sari, İ , Dönmez, G , Kırık, F , Savran, A , Tuğlu, M . (2019). Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi, 6 (2), 74-80. DOI: 10.34087/cbusbed.405472
MLA ÖZKAN, O , Özbey, C , Aydemir, I , Sari, İ , Dönmez, G , Kırık, F , Savran, A , Tuğlu, M . "Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6 (2019): 74-80 <http://dergipark.org.tr/cbusbed/issue/46483/405472>
Chicago ÖZKAN, O , Özbey, C , Aydemir, I , Sari, İ , Dönmez, G , Kırık, F , Savran, A , Tuğlu, M . "Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6 (2019): 74-80
RIS TY - JOUR T1 - Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması AU - OKTAY ÖZKAN , Caner Özbey , Işıl Aydemir , İsmail Sari , Gonca Dönmez , Fatma Esin Kırık , Ahmet Savran , Mehmet İbrahim Tuğlu Y1 - 2019 PY - 2019 N1 - doi: 10.34087/cbusbed.405472 DO - 10.34087/cbusbed.405472 T2 - Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi JF - Journal JO - JOR SP - 74 EP - 80 VL - 6 IS - 2 SN - -2147-9607 M3 - doi: 10.34087/cbusbed.405472 UR - https://doi.org/10.34087/cbusbed.405472 Y2 - 2019 ER -
EndNote %0 CBU-SBED: Celal Bayar University-Health Sciences Institute Journal Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması %A OKTAY ÖZKAN , Caner Özbey , Işıl Aydemir , İsmail Sari , Gonca Dönmez , Fatma Esin Kırık , Ahmet Savran , Mehmet İbrahim Tuğlu %T Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması %D 2019 %J Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi %P -2147-9607 %V 6 %N 2 %R doi: 10.34087/cbusbed.405472 %U 10.34087/cbusbed.405472
ISNAD ÖZKAN, OKTAY , Özbey, Caner , Aydemir, Işıl , Sari, İsmail , Dönmez, Gonca , Kırık, Fatma Esin , Savran, Ahmet , Tuğlu, Mehmet İbrahim . "Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması". Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi 6 / 2 (June 2019): 74-80. https://doi.org/10.34087/cbusbed.405472
AMA ÖZKAN O , Özbey C , Aydemir I , Sari İ , Dönmez G , Kırık F , Savran A , Tuğlu M . Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması. CBU-SBED: Celal Bayar University-Health Sciences Institute Journal. 2019; 6(2): 74-80.
Vancouver ÖZKAN O , Özbey C , Aydemir I , Sari İ , Dönmez G , Kırık F , Savran A , Tuğlu M . Kanser hücreleri üzerine Origanum minutiflorum’un Sitotoksik Etkisinin Araştırılması. Celal Bayar Üniversitesi Sağlık Bilimleri Enstitüsü Dergisi. 2019; 6(2): 80-74.