Year 2018, Volume 2, Issue 1, Pages 21 - 24 2018-06-30

Antibacterial effects of Thymbra spicata L. extracts on Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium and Campylobacter jejuni
Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri

Gamze Çinar [1] , Nurşen Yaman [2] , Muhsin AYDIN [3]

148 307

In this study, the antibacterial effects of extracts acquired from the leaves of zahter (Thymbra spicata L.) plant, a traditional thyme, were investigated on Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium, and Campylobacter jejuni that may cause various damages to human health. For this purpose, diffusion disc assays were performed. Each of the diffusion discs was impregnated with 10 µL of 3 different (10-1, 10-2, and 10-3) dilutions that were diluted from the T. spicata extract homogenates. These discs were placed on the media that was previously cultured with E. coli O157:H7, S. enterica subsp. enterica serovar Typhimurium, and C. jejuni and growth behaviors of each bacteria were observed. According to the obtained results, both E. coli O157:H7 and S. enterica subsp. enterica serovar Typhimurium presented large inhibition zones to 10-1 and 10-2 dilutions (for E. coli O157:H7 21.3±1.7 and 17.7±1.3, respectively; and for S. enterica subsp. enterica serovar Typhimurium: 26.3±1.7 and 20.7±1.3, respectively) but low inhibition zones to 10-3 dilution (8.3±2.1 and 10.0±0.8, respectively). It was observed that the C. jejuni strain formed zones all below 17 mm in all three different dilutions.

Bu çalışmada, zahter (Thymbra spicata L.) bitkisinin yapraklarından elde edilen ekstrakların insan sağlığına çok yönlü zararlar verebilen Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni bakterileri üzerindeki antibakteriyel etkileri araştırılmıştır. Bu amaç doğrultusunda, disk difüzyon yöntemi kullanılmıştır. Steril disklerinin her birine T. spicata ekstraktının homojenatlardan yapılan 3 farklı (10-1, 10-2 ve 10-3) dilüsyondan 10’ar µL emdirilmiş ve bu diskler petri kapları içerisinde bulunan besiyerlerine ekimi yapılmış olan E. coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve C. jejuni bakterilerinin üreyeceği bölgelere bırakılarak üreme davranışları gözlemlenmiştir. Çalışma sonuçlarına göre, yapılan ölçümlerde E. coli O157:H7’nin 10-1 ve 10-2’lik T. spicata ekstraktının bulunduğu etrafında sırasıyla 21,3±1,7 ve 17,7±1,3 mm’lik zonlar oluşturduğu gözlemlenmiştir. Benzer şekilde, S. enterica subsp. enterica serovar Typhimurium da 10-1 ve 10-2’lik T. spicata ekstraktı dilüsyonlarının etrafında sırasıyla 26,3±1,7 ve 20,7±1,3 mm’lik zonlar oluşturmuştur. Fakat hem E. coli O157:H7 hem de Salmonella enterica subsp. enterica serovar Typhimurium 10-3’lük dilüsyonda dar inhibisyon zonları oluşturmuşlardır (sırasıyla 8,3±2,1 ve 10,0±0,8). C. jejuni türünün ise üç farklı dilüsyonun tamamında 17 mm’nin altında kalan zonlar oluşturduğu gözlemlenmiştir.

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Primary Language tr
Subjects Biology
Journal Section Articles
Authors

Author: Gamze Çinar
Institution: Selim Nevzat Şahin Anatolian High School, Ministry of National Education, Hatay, TURKEY
Country: Turkey


Author: Nurşen Yaman
Institution: Selim Nevzat Şahin Anatolian High School, Ministry of National Education, Hatay, TURKEY
Country: Turkey


Orcid: 0000-0002-1204-1163
Author: Muhsin AYDIN (Primary Author)
Institution: Department of Biology, Faculty of Science and Letters, Adiyaman University, Adiyaman, TURKEY
Country: Turkey


Dates

Publication Date: June 30, 2018

Bibtex @research article { commagene399819, journal = {Commagene Journal of Biology}, issn = {}, eissn = {2602-456X}, address = {ABADER (Adıyaman Bilimsel Araştırmalar Derneği)}, year = {2018}, volume = {2}, pages = {21 - 24}, doi = {10.31594/commagene.399819}, title = {Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri}, key = {cite}, author = {Çinar, Gamze and Yaman, Nurşen and AYDIN, Muhsin} }
APA Çinar, G , Yaman, N , AYDIN, M . (2018). Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri. Commagene Journal of Biology, 2 (1), 21-24. DOI: 10.31594/commagene.399819
MLA Çinar, G , Yaman, N , AYDIN, M . "Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri". Commagene Journal of Biology 2 (2018): 21-24 <http://dergipark.org.tr/commagene/issue/37919/399819>
Chicago Çinar, G , Yaman, N , AYDIN, M . "Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri". Commagene Journal of Biology 2 (2018): 21-24
RIS TY - JOUR T1 - Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri AU - Gamze Çinar , Nurşen Yaman , Muhsin AYDIN Y1 - 2018 PY - 2018 N1 - doi: 10.31594/commagene.399819 DO - 10.31594/commagene.399819 T2 - Commagene Journal of Biology JF - Journal JO - JOR SP - 21 EP - 24 VL - 2 IS - 1 SN - -2602-456X M3 - doi: 10.31594/commagene.399819 UR - https://doi.org/10.31594/commagene.399819 Y2 - 2018 ER -
EndNote %0 Commagene Journal of Biology Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri %A Gamze Çinar , Nurşen Yaman , Muhsin AYDIN %T Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri %D 2018 %J Commagene Journal of Biology %P -2602-456X %V 2 %N 1 %R doi: 10.31594/commagene.399819 %U 10.31594/commagene.399819
ISNAD Çinar, Gamze , Yaman, Nurşen , AYDIN, Muhsin . "Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri". Commagene Journal of Biology 2 / 1 (June 2018): 21-24. https://doi.org/10.31594/commagene.399819
AMA Çinar G , Yaman N , AYDIN M . Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri. Comm. J. Biol.. 2018; 2(1): 21-24.
Vancouver Çinar G , Yaman N , AYDIN M . Zahter (Thymbra spicata L.) Bitkisi Ekstraktının, Escherichia coli O157:H7, Salmonella enterica subsp. enterica serovar Typhimurium ve Campylobacter jejuni Üzerindeki Antibakteriyel Etkileri. Commagene Journal of Biology. 2018; 2(1): 24-21.