Misel İyileştirilmiş Katalitik Kinetik Spektrofotometri Aracılığıyla İçecek Örneklerinde Eser Düzeylerdeki Nitritin Belirlenmesi

Yıl 2017, Cilt: 38 Sayı: 3, 400 - 411, 30.09.2017

Adil Elik Nail Altunay Ramazan Gürkan

https://doi.org/10.17776/csj.340413

Cited By: 2

Öz

Bu çalışmada nitrite tayini için basit ve hassas bir katalitik kinetik
spektrofotometre yöntemi anlatılmıştır. Yöntemin temeli, asitli ortamda (520
nm'de emiliminde azalma olur) karışık yüzeyaktif maddeler varlığında nitrit ile
klorpromazinin oksidasyonuna dayanır. Çeşitli kimyasal (asitlik etkisi, reaktif
konsantrasyonları gibi) ve enstrümantal parametreler (zaman, numune hacmi ve
sıcaklık) optimize edilmiştir. Çeşitli kimyasal türlerin girişim etkileri
incelendi. Optimum koşullarda, doğrusal kalibrasyon grafiği, 2.5-125 μg L^-1
nitrit derişimi aralıklarında doğrusal idi. Nitrit için tespit limiti 0.71 μg L^-1
'dir. Nitritin 25 ve 75 μg L^-1 derişimleri için göreceli standart
sapma değerleri sırasıyla % 3.40 ve % 2.35 dir. Misel geliştirilmiş katalitik
kinetik spektrofotometri, iyi hassasiyet, tekrarlanabilirlik, kararlılık ve
nitrite seçicilik de dahil olmak üzere iyi analitik performans göstermiştir.
Yöntemin, içecek örneklerine uygulanması sonucu % 97.3-103.8 aralığında
tatmin edici geri kazanım değerleri elde edilmiştir. Sonuç olarak, önerilen
yöntemin gıda güvenliği için umut verici bir uygulama olduğunu göstermektedir.

Anahtar Kelimeler

Klorpromazin, Nitrit, Katalitik Kinetik, Yüzey Aktif Maddeler, Spektrofotometri, İçecek Örnekleri

Determination of Trace Levels of Nitrite in Beverages Samples Through Micellar Improved Catalytic Kinetic Spectrophotometry

Öz

In this study, a simple and sensitive catalytic kinetic
spectrophotometry method for determination of nitrite has been described. The
method is based on the oxidation of chlorpromazine by nitrite in presence of
mixed surfactants in acidic medium, which results in the decrease in absorbance
at 520 nm. Various chemical (such as the effect of acidity, reagents
concentrations) and instrumental parameters (time, sample volume and
temperature) were optimized. The interfering effects of various chemical
species were studied. At the optimum conditions, linear calibration graph was
linear in the nitrite concentration ranges of 2.5-125 µg L^–1.  The detection limit is 0.71 µg L^–1
for nitrite. The relative standard deviation for determination of 25 and 75 µg
L^–1 were 3.40 and 2.35%, for ten replicate measurements,
respectively. The micellar improved catalytic kinetic spectrophotometry showed good analytical performance
including good sensitivity, reproducibility, stability and selectivity to
nitrite. The method has been applied to determine nitrite in beverages samples
with a satisfactory recovery in the range of 97.3–103.8%, showing its promising
application for food safety monitoring.

Anahtar Kelimeler

Chlorpromazine, Nitrite, Catalytic Kinetic, Surfactants, Spectrophotometry, Beverage Samples

Kaynakça

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