Year 2017, Volume 38, Issue 3, Pages 427 - 434 2017-09-30

Eisenia fetida’dan Elde Edilen Sölom ve Mukus Sıvılarının Antibakteriyel Özelliklerinin Incelenmesi
Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida

Uğur TUTAR [1] , İsa KARAMAN [2]

445 512

Toprak solucanlarının, birçok patojen mikroorganizma ile birlikte yaşarken bunların enfektif etkilerinden güçlü immun sistemleri ve salgıladıkları sekresyonlarla korunabildikleri, ayrıca vermikompost, sölom ve mukus sıvılarının bazı patojen bakterilere karşı etkili oldukları yapılan çeşitli araştırmalarla saptanmıştır. Bu çalışmada, E. fetida türü toprak solucanlarının sölom-mukus sıvılarından elde edilen ekstraktların, bitkilerde hastalıklara neden olan yedi adet toprak kaynaklı patojen bakteriye karşı etkinliklerinin belirlenmesi amacıyla Minimum İnhibisyon Konsantrasyon (MIC) ve Minimum Bakterisidal Konsantrasyon (MBC) testleri uygulanmıştır. Çalışma sonuçlarına göre, toprak solucanlarından elde edilen sölom ve mukus sıvılarının çalışılan bakterilere karşı zon çapları 8-54 mm arasında ölçülürken, MIC değerleri 0.06-0.5 mg/mL, MBC değerleri ise 0.25-2 mg/mL arasında bulunmuştur. Bulgular E.fetida türü toprak solucanlarından elde edilen sölom- mukus sıvılarının bitki patojenleri üzerinde antibakteriyel aktivitelerinin dikkate değer olduğunu göstermektedir. Yapılan bu çalışmanın, E. fetida’dan ve diğer toprak solucanı türlerinden elde edilecek izolat ve ekstraktların antimikrobiyal etkinliklerinin, yapılarının ve etki mekanizmalarının araştırıldığı diğer çalışmalara ışık tutması beklenmektedir. 

In several researches it was found that earthworms are protected from the infective effects of many pathogen microorganisms, with which they live together, by their strong immune systems and secreted fluids; in addition, vermicompost, their coelomic fluid and mucus are effective against certain bacteria. In this study, Disk Diffusion, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) tests were applied to determine the activities of the extracts obtained from coelom-mucus fluids of Eisenia fetida type earthworm against seven pathogenic bacteria causing diseases in plants.  At the end of the study, zone diameters of the coelom-mucus fluids obtained from earthworms were measured to be between 8-54 mm, whereas their MIC and MBC values varied between 0.06-0.5 mg/mL and 0.25-2 mg/mL, respectively. The findings showed that antibacterial activity of coelom-mucus fluids obtained from E. fetida type earthworms on plant pathogens is remarkable. This study is expected to enlighten future studies investigating antimicrobial effects, structure and activity mechanisms of the isolates and extracts obtained from E. fetida and other types of earthworm. 

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Subjects Basic Sciences
Journal Section Articles
Authors

Author: Uğur TUTAR

Author: İsa KARAMAN

Bibtex @research article { csj340474, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2017}, volume = {38}, pages = {427 - 434}, doi = {10.17776/csj.340474}, title = {Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida}, key = {cite}, author = {TUTAR, Uğur and KARAMAN, İsa} }
APA TUTAR, U , KARAMAN, İ . (2017). Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida. Cumhuriyet Science Journal, 38 (3), 427-434. DOI: 10.17776/csj.340474
MLA TUTAR, U , KARAMAN, İ . "Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida". Cumhuriyet Science Journal 38 (2017): 427-434 <http://dergipark.org.tr/csj/issue/31252/340474>
Chicago TUTAR, U , KARAMAN, İ . "Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida". Cumhuriyet Science Journal 38 (2017): 427-434
RIS TY - JOUR T1 - Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida AU - Uğur TUTAR , İsa KARAMAN Y1 - 2017 PY - 2017 N1 - doi: 10.17776/csj.340474 DO - 10.17776/csj.340474 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 427 EP - 434 VL - 38 IS - 3 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.340474 UR - https://doi.org/10.17776/csj.340474 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida %A Uğur TUTAR , İsa KARAMAN %T Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida %D 2017 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 38 %N 3 %R doi: 10.17776/csj.340474 %U 10.17776/csj.340474
ISNAD TUTAR, Uğur , KARAMAN, İsa . "Investigation of Antibacterial Properties of Mucus and Coelomic Fluid Obtained from Eisenia Fetida". Cumhuriyet Science Journal 38 / 3 (September 2017): 427-434. https://doi.org/10.17776/csj.340474