Year 2017, Volume 38, Issue 3, Pages 450 - 460 2017-09-30

Sıçanların Akciğer Dokusunda Oksitosin ve Oksitosin Reseptör Antagonisti Atosiban’ın Karbonik Anhidraz ve Asetilkolinesteraz Enzim Aktiviteleri Üzerine Etkileri
The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats

Ümit M. KOÇYİĞİT [1]

233 290

Çalışmamızda, sıçanlara enjekte edilen oksitosin ve oksitosin reseptör antagonisti olan atosibanın, sıçan akciğer dokusunda karbonik anhidraz (CA) ve asetilkolinesteraz (AChE) enzim aktiviteleri üzerine etkilerinin incelenerek, oksitosinin canlı metabolizmadaki rolünün tam olarak anlaşılmasına yardımcı olmak amaçlanmıştır. Bu amaçla, her biri 6 adet sıçandan oluşan 4 farklı grup oluşturuldu.  (Kontrol grubu, oksitosin verilen grup, atosiban verilen grup ve oksitosin ile atosibanın birlikte verildiği grup). Sıçanlara kimyasallar intraperitoneal olarak enjekte edildikten bir saat sonra sıçanlar nekropsiye alındı. Sıçanların akciğer dokuları çıkarıldı. Bu dokular için CA ve AChE aktiviteleri ölçüldü. Tüm deneysel sonuçlar ortalama±SH (ortalamanın standart hatası) olarak ifade edildi. Veriler normal dağılıma uygunluk gösterdiği için varyans analizi (ANOVA) ile test edildi ve daha sonra anlamlılık Tukey test ile belirlendi. İstatistiksel anlamlılık p<0.05 düzeyinde tanımlandı. Buna göre, oksitosin ve atosiban verilen gruplarda enzim aktivelerinde azalma, oksitosin ile atosibanın birlikte verildiği gruptaki enzim aktivelerinde ise anlamlı bir değişiklik olmadığı görüldü.Ayrıca Oksitosinin sistemik bir etki olarak CA enzim aktivitesini azalttığını gözlemledik. Bu sonucun akciğerle alakalı olan akciğer oksijen karbondioksit taşınmasında bozuklukla seyreden hastalıklara katkısı olabileceğini düşünmekteyiz.

The purpose of our studies is to assist in fully understanding the role of oxytocin in the live metabolism by examining the effects of oxytocin, oxytocin receptor antagonist atosiban, and oxytocin-atosiban chemicals injected to the rats on activities of carbonic anhydrase (CA) and acetylcholinesterase (AChE) enzymes in the lung tissue of the rats. For this purpose, 4 different groups each of which contains 6 rats formed. (Control group, oxytocin administered group, atosiban administered group, and both oxytocin and atosiban administered group). The rats were necropsied after an hour from the injection of chemicals into the rats intraperitoneally. The lung tissues of the rats were extracted. Carbonic anhydrase and acetylcholinesterase enzymes activities were measured for the tissues.  All the experimental results were provided as mean ± SD (mean standard deviation). Because of the data was compatible with the normal range, it was tested via variance analysis (ANOVA) and then, significance was determined by the Tukey test. Statistical significance was identified to be p <0.05.

Accordingly, it was observed that there was neither a decrease of enzyme activities in oxytocin and atosiban administered groups, nor a significant change of enzyme activities in both oxytocin and atosiban administered group. Also, we observed that oxytocin reduced carbonic anhydrase enzyme activity as a systemic effect. We think that this result may contribute to remedy of lung related diseases progressing with oxygen and carbon dioxide transport disorders. 

  • [1] Kelly, J., & Swanson, L.W. (1980). Additional forebrain regions projecting to the posterior pituitary: preoptic region, bed nucleus of the Stria terminalis, and Zona incerta. Brain research, 197(1), 1-9.
  • [2] Arletti, R., Benelli, A., & Bertolini, A. (1989). Influence of oxytocin on feeding behavior in the rat. Peptides, 10(1), 89-93.
  • [3] Sanu, O., & Lamont, R. F. (2010). Critical appraisal and clinical utility of atosiban in the management of preterm labor. Therapeutics and Clinical Risk Management, 6, 191.
  • [4] Berg, J.M.,Tymoczko, J.L., Stryer, L. (2014). Biyokimya. Palme Yayıncılık.241-247p,
  • [5] Gocer, H., Topal, F., Topal, M., Küçük, M., Teke, D., Gülçin, İ., ... & Supuran, C. T. (2016). Acetylcholinesterase and carbonic anhydrase isoenzymes I and II inhibition profiles of taxifolin. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(3), 441-447.
  • [6] Kocyigit, U. M., Aslan, O. N., Gulcin, I., Temel, Y., & Ceylan, M. (2016). Synthesis and carbonic anhydrase inhibition of novel 2-(4-(aryl)thiazole-2-yl)-3a,4,7,7a-tetrahydro-1h-4,7-methanoisoindole-1,3(2h)-dione derivatives. Archiv der Pharmazie, 349(12), 955-963.
  • [7] Gul, H. I., Mete, E., Taslimi, P., Gulcin, I., & Supuran, C. T. (2017). Synthesis, carbonic anhydrase I and II inhibition studies of the 1, 3, 5-trisubstituted-pyrazolines. Journal of Enzyme Inhibition And Medicinal Chemistry, 32(1), 189-192.
  • [8] Kucukoglu, K., Oral, F., Aydin, T., Yamali, C., Algul, O., Sakagami, H., ... & Gul, H. I. (2016). Synthesis, cytotoxicity and carbonic anhydrase inhibitory activities of new pyrazolines. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(S4), 20-24.
  • [9] Bhatt, A., Mahon, B. P., Cruzeiro, V. W. D., Cornelio, B., Laronze Cochard, M., Ceruso, M., & Roitberg, A. (2017). Structure–activity relationships of benzenesulfonamide based inhibitors towards carbonic anhydrase isoform specificity. Chembiochem, 18(2), 213-222.
  • [10] Akıncıoğlu, A., Topal, M., Gulcin, I., & Göksu, S. (2014). Novel sulphamides and sulphonamides incorporating the tetralin scaffold as carbonic anhydrase and acetylcholine esterase inhibitors. Archiv der Pharmazie, 347(1), 68-76.
  • [11] Göçer, H., Akincioğlu, A., Göksu, S., Gülçin, İ., & Supuran, C. T. (2015). Carbonic anhydrase and acetylcholine esterase inhibitory effects of carbamates and sulfamoylcarbamates. Journal of Enzyme Inhibition and Medicinal Chemistry, 30(2), 316-320.
  • [12] Polat Köse, L., Gülçin, İ., Gören, A.C., Namiesnik, J., Martinez-Ayala, A. L., & Gorinstein, S. (2015). LC–MS/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes. Industrial Crops and Products, 74, 712-721.
  • [13] Özbey, F., Taslimi, P., Gülçin, İ., Maraş, A., Göksu, S., & Supuran, C. T. (2016). Synthesis of diaryl ethers with acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase inhibitory actions. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(S2), 79-85.
  • [14] Taslimi, P., Gulcin, I., Ozgeris, B., Goksu, S., Tumer, F., Alwasel, S. H., & Supuran, C. T. (2016). The human carbonic anhydrase isoenzymes I and II (hCA I and II) inhibition effects of trimethoxyindane derivatives. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(1), 152-157.
  • [15] Taslimi, P., Osmanova, S., Gulçin, İ., Sardarova, S., Farzaliyev, V., Sujayev, A., ... & Kufrevioglu, O. I. (2017). Discovery of potent carbonic anhydrase, acetylcholinesterase, and butyrylcholinesterase enzymes inhibitors: The new amides and thiazolidine-4-ones synthesized on an acetophenone base. Journal of Biochemical and Molecular Toxicology, DOI: 10.1002/jbt.21931.
  • [16] Taslimi, P., Sujayev, A., Mamedova, S., Kalın, P., Gulçin, İ., Sadeghian, N., ... & Mamedov, S. (2017). Synthesis and bioactivity of several new hetaryl sulfonamides. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 137-145.
  • [17] Gül, H. I., Demirtas, A., Ucar, G., Taslimi, P., Gulcin, I. (2017). Synthesis of Mannich bases by two different methods and evaluation of their acetylcholine esterase and carbonic anhydrase inhibitory activities. Letters in Drug Design and Discovery, 14(5), 573-580.
  • [18] Aksu, K., Özgeriş, B., Taslimi, P., Naderi, A., Gülçin, İ., & Göksu, S. (2016). Antioxidant activity, acetylcholinesterase, and carbonic anhydrase ınhibitory properties of novel ureas derived from phenethylamines. Archiv der Pharmazie, 349(12), 944-954.
  • [19] Garibov, E., Taslimi, P., Sujayev, A., Bingol, Z., Çetinkaya, S., Gulçin, İ., ... & Supuran, C. T. (2016). Synthesis of 4, 5-disubstituted-2-thioxo-1, 2, 3, 4-tetrahydropyrimidines and investigation of their acetylcholinesterase, butyrylcholinesterase, carbonic anhydrase I/II inhibitory and antioxidant activities. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(sup3), 1-9.
  • [20] Özbey, F., Taslimi, P., Gülçin, İ., Maraş, A., Göksu, S., & Supuran, C. T. (2016). Synthesis of diaryl ethers with acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase inhibitory actions. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(S2), 79-85.
  • [21] Turan, B., Şendil, K., Şengül, E., Gültekin, M. S., Taslimi, P., Gulçin, İ., & Supuran, C. T. (2016). The synthesis of some β-lactams and investigation of their metal-chelating activity, carbonic anhydrase and acetylcholinesterase inhibition profiles. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(S1), 79-88.
  • [22] Gul, H. I., Tugrak, M., Sakagami, H., Taslimi, P., Gulcin, I., & Supuran, C. T. (2016). Synthesis and bioactivity studies on new 4-(3-(4-Substitutedphenyl)-3a, 4-dihydro-3 H-indeno [1,2-c] pyrazol-2-yl) benzenesulfonamides Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1619-1624.
  • [23] Gul, H. I., Kucukoglu, K., Yamali, C., Bilginer, S., Yuca, H., Ozturk, I., ... & Supuran, C. T. (2016). Synthesis of 4-(2-substituted hydrazinyl) benzenesulfonamides and their carbonic anhydrase inhibitory effects. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(4), 568-573.
  • [24] Sujayev, A., Garibov, E., Taslimi, P., Gulçin, İ., Gojayeva, S., Farzaliyev, V., ... & Supuran, C. T. (2016). Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1531-1539.
  • [25] Gülçin, İ., Scozzafava, A., Supuran, C. T., Koksal, Z., Turkan, F., Çetinkaya, S., ... & Alwasel, S. H. (2016). Rosmarinic acid inhibits some metabolic enzymes including glutathione S-transferase, lactoperoxidase, acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase isoenzymes Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1698-1702.
  • [26] Innocenti, A., Sarıkaya, S. B. Ö., Gülçin, I., & Supuran, C. T. (2010). Carbonic anhydrase inhibitors. Inhibition of mammalian isoforms I–XIV with a series of natural product polyphenols and phenolic acids. Bioorganic & Medicinal Chemistry, 18(6), 2159-2164.
  • [27] Innocenti, A., Gülçin, I., Scozzafava, A., & Supuran, C.T. (2010). Carbonic anhydrase inhibitors. Antioxidant polyphenols effectively inhibit mammalian isoforms I–XV. Bioorganic & Medicinal Chemistry Letters, 20(17), 5050-5053.
  • [28] Güney, M., Coşkun, A., Topal, F., Daştan, A., Gülçin, İ., & Supuran, C. T. (2014). Oxidation of cyanobenzocycloheptatrienes: synthesis, photooxygenation reaction and carbonic anhydrase isoenzymes inhibition properties of some new benzotropone derivatives. Bioorganic & Medicinal Chemistry, 22(13), 3537-3543.
  • [29] Scozzafava, A., Kalın, P., Supuran, C. T., Gülçin, İ., & Alwasel, S. H. (2015). The impact of hydroquinone on acetylcholine esterase and certain human carbonic anhydrase isoenzymes (hCA I, II, IX, and XII). Journal of Enzyme Inhibition and Medicinal Chemistry, 30(6), 941-946.
  • [30] Gülçin, İ., Beydemir, Ş., & Büyükokuroğlu, M. E. (2004). In vitro and in vivo effects of dantrolene on carbonic anhydrase enzyme activities. Biological and Pharmaceutical Bulletin, 27(5), 613-616.
  • [31] Artunç, T., Çetinkaya, Y., Göçer, H., Gülçin, İ., Menzek, A., Şahin, E., Supuran, C. T. (2015). Synthesis of 4-[2-(3,4-dimethoxybenzyl)cyclopentyl]-1,2- dimethoxybenzene derivatives and evaluations of their carbonic anhydrase isoenzymes inhibitory effects. Chemical Biology & Drug Design, 87(4):594-607.
  • [32] Aksu, K., Özgeriş, B., Taslimi, P., Naderi, A., Gülçin, İ., & Göksu, S. (2016). Antioxidant Activity, Acetylcholinesterase, and Carbonic Anhydrase Inhibitory Properties of Novel Ureas Derived from Phenethylamines. Archiv der Pharmazie, 349(12), 944-954.
  • [33] Gul, H. I., Tugrak, M., Sakagami, H., Taslimi, P., Gulcin, I., & Supuran, C. T. (2016). Synthesis and bioactivity studies on new 4-(3-(4-Substitutedphenyl)-3a, 4-dihydro-3 H-indeno [1, 2-c] pyrazol-2-yl) benzenesulfonamides. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1619-1624.
  • [34] Göçer, H., Akıncıoğlu, A., Öztaşkın, N., Göksu, S., & Gülçin, İ. (2013). Synthesis, antioxidant, and antiacetylcholinesterase activities of sulfonamide derivatives of dopamine-related compounds. Archiv der Pharmazie, 346(11), 783-792.
  • [35] Akbaba, Y., Bastem, E., Topal, F., Gülçin, İ., Maraş, A., & Göksu, S. (2014). synthesis and carbonic anhydrase inhibitory effects of novel sulfamides derived from 1-aminoindanes and anilines. Archiv der Pharmazie, 347(12), 950-957.
  • [36] Camadan, Y., Özdemir, H., & Gulcin, İ. (2016). Purification and characterization of dihydropyrimidine dehydrogenase enzyme from sheep liver and determination of the effects of some anaesthetic and antidepressant drugs on the enzyme activity. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1335-1341.
  • [37] Sujayev, A., Garibov, E., Taslimi, P., Gulçin, İ., Gojayeva, S., Farzaliyev, V., ... & Supuran, C. T. (2016). Synthesis of some tetrahydropyrimidine-5-carboxylates, determination of their metal chelating effects and inhibition profiles against acetylcholinesterase, butyrylcholinesterase and carbonic anhydrase. Journal of Enzyme Inhibition and Medicinal Chemistry, 31(6), 1531-1539.
  • [38] H. I. Gül, K. Kucukoglu, C. Yamali, S. Bilginer, H. Yuca, I. Ozturk, P.Taslimi, I. Gülçin, C. T. Supuran, (2016). Synthesis of 4-(2-substitutedhydrazinyl)benzenesulfonamides and their carbonic anhydrase inhibitory effects. Journal of Enzyme Inhibition and Medicinal Chemistry, 31,568-573.
  • [39] Gul, H. I., Mete, E., Taslimi, P., Gulcin, I., & Supuran, C. T. (2017). Synthesis, carbonic anhydrase I and II inhibition studies of the 1,3,5-trisubstituted-pyrazolines. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 189-192.
  • [40] Ceylan, M., Kocyigit, U. M., Usta, N.C., Gürbüzlü, B., Temel, Y., Alwasel, S.H., & Gülçin, İ. (2016). Synthesis, carbonic anhydrase I and II isoenzymes inhibition properties, and antibacterial activities of novel tetralone-based 1,4-benzothiazepine derivatives. Journal of Biochemical and Molecular Toxicology, 31, e21872.
  • [41] Topal, F., Gulcin, I., Dastan, A., & Guney, M., (2017). Novel eugenol derivatives: Potent acetylcholinesterase and carbonic anhydrase inhibitors. International Journal of Biological Macromolecules, 94, 845-851.
  • [42] Kocyigit, U. M., Budak, Y., Gürdere, M. B., Tekin, Ş., Köprülü, T. K., Ertürk, F., ... & Ceylan, M., (2016). Synthesis, characterization, anticancer, antimicrobial and carbonic anhydrase ınhibition profiles of novel (3aR, 4S, 7R, 7aS)-2-(4-((E)-3-(3-aryl) acryloyl) phenyl)-3a, 4, 7, 7a-tetrahydro-1H-4, 7-methanoisoindole-1, 3 (2H)-dione derivatives. Bioorganic Chemistry, 70, 118-125.
  • [43] Göksu, S., Naderi, A., Akbaba, Y., Kalın, P., Akıncıoğlu, A., Gülçin, İ., ... & Salmas, R. E. (2014). Carbonic anhydrase inhibitory properties of novel benzylsulfamides using molecular modeling and experimental studies. Bioorganic Chemistry, 56, 75-82.
  • [44] Erbas O, Yılmaz M, Anil H, Bora S, Evren V, Peker G., (2013). Oxytocin inhibits pentylentetrazol-induced seizures in the rat. Peptides, 40:141-144.
  • [45] Simsek Y, Celik O, Karaer A, et al., (2012) Elevated cardiac oxidative stress in newborn rats from mothers treated with atosiban, 655-661.
  • [46] Wistrand, P.J., (2002). Carbonic anhydrase III in liver and muscle of male rats purification and properties. Upsala Journal of Medicinal Sciences, 107, 77-88.
  • [47] Kocyigit UM, Taskıran A, Taslimi P,Yokus A, Gulcın I, (2017). Inhibitory effects of oxytocin and oxytocin receptor antagonist atosiban on the activities of carbonic anhydrase and acetylcholinesterase enzymes in the liver and kidney tissues of rats. Journal of Biochemical and Molecular Toxicology, DOI: 10.1002/jbt.21972.
  • [48] Pullan, L.M. and Noltmann, E.A., (1985). Purification and properties of pig muscle carbonic anhydrase III. Biochimica et Biophysica Acta, 839, 147-54.
  • [49] Wilbur, K.M. and Anderson, N.G., (1948). Electrometric and colorimetric determination of carbonic anhydrase. Journal of Biological Chemistry, 176, 147-154.
  • [50] Rıcklı, E.E., Ghazanfar, S.A.S., Gibbons, B.H. and Edsall, J.T., (1964). Carbonic anhydrase from human erythrocytes. Journal of Biological Chemistry, 239, 1065
  • [51] Maren, T. H., (1960). A simplified micro method for the determination of carbonic anhydrase and its inhibitors. Journal of Pharmacology and Experimental Therapeutics, 130, 26.
  • [52] Armstrong, J. M., Myers, D.V., Verpoorte, J.A. and Edsall, J.T., (1966). Purification and properties of human erythrocyte carbonic anhydrase. Journal of Biological Chemistry 214, 5137.
  • [53] Verpoorte, J. A., Mehta S, Edsall J.T. (1967). Esterase Activities of Human Carbonic Anhydrases B and C Journal of Biological Chemistry 242, 4221-4229.
  • [54] Ellman, G. L., Courtney, K. D., Andres, V., & Featherstone, R. M. (1961). A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical pharmacology, 7(2), 88IN191-9095.
Subjects Basic Sciences
Journal Section Articles
Authors

Author: Ümit M. KOÇYİĞİT
Country: Turkey


Bibtex @research article { csj340483, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2017}, volume = {38}, pages = {450 - 460}, doi = {10.17776/csj.340483}, title = {The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats}, key = {cite}, author = {KOÇYİĞİT, Ümit M.} }
APA KOÇYİĞİT, Ü . (2017). The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats. Cumhuriyet Science Journal, 38 (3), 450-460. DOI: 10.17776/csj.340483
MLA KOÇYİĞİT, Ü . "The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats". Cumhuriyet Science Journal 38 (2017): 450-460 <http://dergipark.org.tr/csj/issue/31252/340483>
Chicago KOÇYİĞİT, Ü . "The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats". Cumhuriyet Science Journal 38 (2017): 450-460
RIS TY - JOUR T1 - The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats AU - Ümit M. KOÇYİĞİT Y1 - 2017 PY - 2017 N1 - doi: 10.17776/csj.340483 DO - 10.17776/csj.340483 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 450 EP - 460 VL - 38 IS - 3 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.340483 UR - https://doi.org/10.17776/csj.340483 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats %A Ümit M. KOÇYİĞİT %T The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats %D 2017 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 38 %N 3 %R doi: 10.17776/csj.340483 %U 10.17776/csj.340483
ISNAD KOÇYİĞİT, Ümit M. . "The Effects of Oxytocin and Oxytocin Receptor Antagonist Atosiban on the Carbonic Anhydrase and Acetylcholinesterase Enzymes from Lung Tissues of Rats". Cumhuriyet Science Journal 38 / 3 (September 2017): 450-460. https://doi.org/10.17776/csj.340483