Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. Sulu Ekstrelerinin in vitro İnsan Periferal Kan Lenfositleri Üzerindeki Sitotoksik ve Genotoksik Etkileri

Yıl 2018, Cilt: 39 Sayı: 1, 127 - 143, 16.03.2018

Tülay Aşkın Çelik Özlem Sultan Aslantürk

https://doi.org/10.17776/csj.405629

Cited By: 1

Öz

Bu çalışmanın amacı Rosmarinus officinalis, Lavandula
stoechas L. ve Tilia cordata Mill'den elde edilen sulu ekstrelerin
(infüzyon ve dekoksiyon), insan periferal kan lenfositleri üzerindeki in vitro
sitotoksik ve genotoksik etkisinin araştırılmasıdır.

Bu amaçla, 3 kadından alınan lenfositler, R.
officinalis (1.5 ve 3 mg / ml), L. stoechas (0,4 ve 1,2 mg/ ml) ve T.
cordata (1 mg / ml ve 3 mg / ml) sulu ekstreleri ile 48 saat süreyle
muamele edilmiştir. Denemelerde PBS negatif kontrol olarak, Mitomisin C (MMC)
(0,25 μg / ml) ise pozitif kontrol olarak kullanılmıştır. Ekstrelerin lenfositlerdeki
sitotoksik etkisi, mitotik indeks hesaplanarak belirlenmiştir. Ayrıca,
lenfositlerideki kromozom aberasyonları ve mikronukleus oluşumları da
belirlenmiştir.

Deneyde kullanılan sulu ekstreler, kontrol
gruplarına kıyasla konsantrasyon artışına bağlı olarak, periferal lenfosit
hücrelerinde bölünmeyi önemli ölçüde azaltmıştır (p <0,01). Yine,
metafazdaki hücrelerin sayısı da azalmıştır. Bu nedenle, periferal
lenfositlerde kromozom incelemesi yapılamamıştır. Bununla birlikte, sulu ekstre
muameleleri periferal lenfositlerde kontrol gruplarına kıyasla mikronukleus
oluşumuna neden olmıuştur (p <0,01).

L. stoechas
ve T. cordata'nın 3 mg / ml dekoksiyon ekstreleri, 48 saatlik muameleden
sonra lenfositler üzerinde en yüksek sitotoksik etkiyi göstermiştir. En yüksek
genotoksik etki ise 1.2 mg / ml L. stoechas dekoksiyon ekstresi ile
muameleden sonra ortaya çıkmıştır.

Anahtar Kelimeler

Sitotoksik etki, genotoksik etki, insan periferal kan lenfositleri, Lavandula stoechas, Rosmarinus officinalis, Tilia cordata

Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes

Öz

The aim of this study was to investigation of in
vitro cytotoxic and genotoxic effects of infusion and decoction aqueous
extracts obtained from Rosmarinus officinalis, Lavandula stoechas
L. and Tilia cordata Mill. on human peripheral blood lymphocytes.

For this purpose, lymphocytes taken from 3
females, were treated with aqueous extracts of R. officinalis (1.5 and 3
mg/ml),  L. stoechas (0,4 and 1,2
mg/ml) and T. cordata (1 mg/ml and 3 mg/ml) for 48 hours. PBS was used
as negative control and Mitomycin C (MMC) (0,25 μg/ml) was used as positive
control in experiments. Cytotoxic effect of extracts on lymphocytes was
determined by calculating mitotic index. Also, chromosome aberrations
micronucleus formations were determined. 

All extracts considerably decreased the
cell division in lymphocytes depending on the increased concentration in
comparison with control groups (p<0,01). Also cells in metaphase decreased.
Thus, no chromosomal aberration was observed in lymphocytes. However, extract
treatments induced the formation of micronucleus in lymphocytes when compared
to control groups (p<0,01). 3 mg/ml decoction extracts of L. stoechas and
T. cordata showed the highest cytotoxic effect on lymphocytes, and the
highest genotoxic effect appeared after 48 h treatment with 1.2 mg/ml decoction
extract of L. stoechas. 

Anahtar Kelimeler

Cytotoxic effect, genotoxic effect, human peripheral lymphocytes, Lavandula stoechas, Rosmarinus officinalis, Tilia cordata

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