Year 2018, Volume 39, Issue 1, Pages 127 - 143 2018-03-16

Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes
Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. Sulu Ekstrelerinin in vitro İnsan Periferal Kan Lenfositleri Üzerindeki Sitotoksik ve Genotoksik Etkileri

Tülay AŞKIN ÇELİK [1] , Özlem Sultan ASLANTÜRK [2]

167 268

The aim of this study was to investigation of in vitro cytotoxic and genotoxic effects of infusion and decoction aqueous extracts obtained from Rosmarinus officinalis, Lavandula stoechas L. and Tilia cordata Mill. on human peripheral blood lymphocytes.

For this purpose, lymphocytes taken from 3 females, were treated with aqueous extracts of R. officinalis (1.5 and 3 mg/ml),  L. stoechas (0,4 and 1,2 mg/ml) and T. cordata (1 mg/ml and 3 mg/ml) for 48 hours. PBS was used as negative control and Mitomycin C (MMC) (0,25 μg/ml) was used as positive control in experiments. Cytotoxic effect of extracts on lymphocytes was determined by calculating mitotic index. Also, chromosome aberrations micronucleus formations were determined. 

All extracts considerably decreased the cell division in lymphocytes depending on the increased concentration in comparison with control groups (p<0,01). Also cells in metaphase decreased. Thus, no chromosomal aberration was observed in lymphocytes. However, extract treatments induced the formation of micronucleus in lymphocytes when compared to control groups (p<0,01). 3 mg/ml decoction extracts of L. stoechas and T. cordata showed the highest cytotoxic effect on lymphocytes, and the highest genotoxic effect appeared after 48 h treatment with 1.2 mg/ml decoction extract of L. stoechas

Bu çalışmanın amacı Rosmarinus officinalis, Lavandula stoechas L. ve Tilia cordata Mill'den elde edilen sulu ekstrelerin (infüzyon ve dekoksiyon), insan periferal kan lenfositleri üzerindeki in vitro sitotoksik ve genotoksik etkisinin araştırılmasıdır.

Bu amaçla, 3 kadından alınan lenfositler, R. officinalis (1.5 ve 3 mg / ml), L. stoechas (0,4 ve 1,2 mg/ ml) ve T. cordata (1 mg / ml ve 3 mg / ml) sulu ekstreleri ile 48 saat süreyle muamele edilmiştir. Denemelerde PBS negatif kontrol olarak, Mitomisin C (MMC) (0,25 μg / ml) ise pozitif kontrol olarak kullanılmıştır. Ekstrelerin lenfositlerdeki sitotoksik etkisi, mitotik indeks hesaplanarak belirlenmiştir. Ayrıca, lenfositlerideki kromozom aberasyonları ve mikronukleus oluşumları da belirlenmiştir.

Deneyde kullanılan sulu ekstreler, kontrol gruplarına kıyasla konsantrasyon artışına bağlı olarak, periferal lenfosit hücrelerinde bölünmeyi önemli ölçüde azaltmıştır (p <0,01). Yine, metafazdaki hücrelerin sayısı da azalmıştır. Bu nedenle, periferal lenfositlerde kromozom incelemesi yapılamamıştır. Bununla birlikte, sulu ekstre muameleleri periferal lenfositlerde kontrol gruplarına kıyasla mikronukleus oluşumuna neden olmıuştur (p <0,01).

L. stoechas ve T. cordata'nın 3 mg / ml dekoksiyon ekstreleri, 48 saatlik muameleden sonra lenfositler üzerinde en yüksek sitotoksik etkiyi göstermiştir. En yüksek genotoksik etki ise 1.2 mg / ml L. stoechas dekoksiyon ekstresi ile muameleden sonra ortaya çıkmıştır.

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Primary Language en
Subjects Basic Sciences
Journal Section Natural Sciences
Authors

Author: Tülay AŞKIN ÇELİK (Primary Author)

Author: Özlem Sultan ASLANTÜRK

Bibtex @research article { csj405629, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2018}, volume = {39}, pages = {127 - 143}, doi = {10.17776/csj.405629}, title = {Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes}, key = {cite}, author = {AŞKIN ÇELİK, Tülay and ASLANTÜRK, Özlem Sultan} }
APA AŞKIN ÇELİK, T , ASLANTÜRK, Ö . (2018). Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes. Cumhuriyet Science Journal, 39 (1), 127-143. DOI: 10.17776/csj.405629
MLA AŞKIN ÇELİK, T , ASLANTÜRK, Ö . "Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes". Cumhuriyet Science Journal 39 (2018): 127-143 <http://dergipark.org.tr/csj/issue/36110/405629>
Chicago AŞKIN ÇELİK, T , ASLANTÜRK, Ö . "Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes". Cumhuriyet Science Journal 39 (2018): 127-143
RIS TY - JOUR T1 - Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes AU - Tülay AŞKIN ÇELİK , Özlem Sultan ASLANTÜRK Y1 - 2018 PY - 2018 N1 - doi: 10.17776/csj.405629 DO - 10.17776/csj.405629 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 127 EP - 143 VL - 39 IS - 1 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.405629 UR - https://doi.org/10.17776/csj.405629 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes %A Tülay AŞKIN ÇELİK , Özlem Sultan ASLANTÜRK %T Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes %D 2018 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 39 %N 1 %R doi: 10.17776/csj.405629 %U 10.17776/csj.405629
ISNAD AŞKIN ÇELİK, Tülay , ASLANTÜRK, Özlem Sultan . "Cytotoxic and Genotoxic Effects of Aqueous Extracts of Rosmarinus officinalis L., Lavandula stoechas L. and Tilia cordata Mill. on in vitro Human Peripheral Blood Lymphocytes". Cumhuriyet Science Journal 39 / 1 (March 2018): 127-143. https://doi.org/10.17776/csj.405629