Year 2018, Volume 39, Issue 1, Pages 169 - 180 2018-03-16

Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri
Cytotoxic, Genotoxic and Oxidative Effects of Cladonia furcata (Huds.) Schrad. on Human Peripheral Lymphocytes

Buğrahan EMSEN [1] , Ali ASLAN [2] , Abdullah KAYA [3]

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Mevcut çalışmada, Cladonia furcata (Huds.) Schrad.  likeninden elde edilen metanol ve su ekstraktlarının (sırasıyla CME ve CSE) insan lenfositleri üzerindeki aktivitelerinin değerlendirilmesi amaçlanmıştır. Lenfositlerdeki sitotoksik etkiyi test etmek için 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz testleri kullanılmıştır. Ayrıca, hücrelerdeki oksidatif stres ve genotoksisite değişimleri sırasıyla toplam oksidan durum ve 8-hidroksi-2'-deoksiguanozin seviyeleri ile incelenmiştir. Bu uygulamalara ek olarak, ekstraktlar ile muamele edilen lenfositlerdeki toplam antioksidan kapasite (TAK) değişimleri de belirlenmiştir. CSE'nin düşük konsantrasyonlarının yüksek seviyelerde sitotoksik etki göstermedikleri ortaya çıkarılmıştır (IC50=221,14 mg/L). Gerçekleştirilen korelasyon analizleri sitotoksisitenin oksidatif stres ile ilişkili (p < 0.01) olduğunu göstermiştir. Tüm uygulamalar göz önüne alındığında, özellikle CSE'nin negatif kontrole kıyasla hücreler üzerinde istatistiksel olarak (p >0,0,5) genetik hasar meydana getirmediği tespit edilmiştir. Hücrelerdeki TAK temel alındığında, ekstraktların belli konsantrasyonlarının (12,5-50 mg/L) TAK'yi anlamlı derecede (p <0,05) yükselttiği dikkat çekmektedir. Sonuç olarak elde edilen veriler, özellikle CSE'nin lenfositler üzeride genotoksik ve oksidatif strese neden olmayarak, yüksek antioksidan özellikleri sayesinde yeni bir tedavi maddesi kaynağı olabileceğini ortaya çıkarmıştır.

In the present study, it was aimed the evaluation of activities of methanol and water extracts (respectively, CME and CSE) obtained from Cladonia furcata (Huds.) Schrad. on human lymphocytes. In order to test cytotoxic effect in lymphocytes, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and lactate dehydrogenase tests were used. Oxidative stress and genotoxicity changes in the cells were also examined with total oxidant status and 8-hydroxy-2-deoxyguanosine levels, respectively. In addition to these assays, total antioxidant capacity (TAC) changes in lymphocytes treated with extracts were also determined. It was revealed that low concentrations of CSE did not show cytotoxic effect at high levels (IC50=221,14 mg/L). Performed correlation analyzes showed that cytotoxicity was associated with oxidative stress (p < 0,01). Considering all applications, it was determined that CSE did not statistically (p > 0,05) cause genetic damage on cells compared to negative control. Based on TAC in cells, it is notable that certain concentrations of the extracts (12,5-50 mg/L) significantly increased TAC (p < 0,05). Consequently, the resulting data reveal that especially CSE can be a source of new therapeutic agents due to its high antioxidant properties, without causing genotoxic and oxidative stress on lymphocytes.

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Primary Language tr
Subjects Basic Sciences
Journal Section Natural Sciences
Authors

Author: Buğrahan EMSEN (Primary Author)

Author: Ali ASLAN

Author: Abdullah KAYA

Bibtex @research article { csj405735, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2018}, volume = {39}, pages = {169 - 180}, doi = {10.17776/csj.405735}, title = {Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri}, key = {cite}, author = {EMSEN, Buğrahan and ASLAN, Ali and KAYA, Abdullah} }
APA EMSEN, B , ASLAN, A , KAYA, A . (2018). Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri. Cumhuriyet Science Journal, 39 (1), 169-180. DOI: 10.17776/csj.405735
MLA EMSEN, B , ASLAN, A , KAYA, A . "Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri". Cumhuriyet Science Journal 39 (2018): 169-180 <http://dergipark.org.tr/csj/issue/36110/405735>
Chicago EMSEN, B , ASLAN, A , KAYA, A . "Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri". Cumhuriyet Science Journal 39 (2018): 169-180
RIS TY - JOUR T1 - Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri AU - Buğrahan EMSEN , Ali ASLAN , Abdullah KAYA Y1 - 2018 PY - 2018 N1 - doi: 10.17776/csj.405735 DO - 10.17776/csj.405735 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 169 EP - 180 VL - 39 IS - 1 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.405735 UR - https://doi.org/10.17776/csj.405735 Y2 - 2017 ER -
EndNote %0 Cumhuriyet Science Journal Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri %A Buğrahan EMSEN , Ali ASLAN , Abdullah KAYA %T Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri %D 2018 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 39 %N 1 %R doi: 10.17776/csj.405735 %U 10.17776/csj.405735
ISNAD EMSEN, Buğrahan , ASLAN, Ali , KAYA, Abdullah . "Cladonia furcata (Huds.) Schrad.'nın İnsan Periferal Lenfositleri Üzerindeki Sitotoksik, Genotoksik ve Oksidatif Etkileri". Cumhuriyet Science Journal 39 / 1 (March 2018): 169-180. https://doi.org/10.17776/csj.405735