Bazı 2-aminotiazol Türevlerine Maruz Kalmış Glutatyon Redüktaz Aktivitesindeki Değişimlerinin Belirlenmesi

Yıl 2019, Cilt: 40 Sayı: 1, 136 - 140, 22.03.2019

Hasan Karadağ Emine Eroğlu Cumhur Kırılmış

https://doi.org/10.17776/csj.504690

Cited By: 1

Öz

Bu çalışmada, 2-Aminotiazol türevleri olan
4,4’-(disulfanediylbis(methylene))bis(thiazol-2-amine) dihyrochloride (DMTA) ve
2-amino-4-(chloromethyl)thiazole hydrochloride (ACT)’in 0 dan 500 mg/L ye
değişen derişimlerinin ekmek mayası (Saccharomyces
cerevisiae) glutatyon redüktazı (GR) üzerine olan etkileri araştırılmıştır.
25, 50, 100, 250 ve 500 mg/L derişimlere maruz bırakılma ile % GR
aktivitesindeki değişimler, DMTA uygulamalarında sırasıyla -5,29 ; -3,85; -2,40
; -6,73 ve -10,58 olarak hesaplanırken, ACT uygulamalarında sırasıyla +0,98;
0,00; -0,49; -2,45 ve 0,00 olarak hesaplanmıştır. Bu çalışma, DMTA
derişimlerinin artışı ile GR aktivitesinde hafif bir düşüş olduğunu ve ACT
derişimlerinin artışı ile GR aktivitesinde önemli bir değişimin olmadığını
göstermiştir. Ancak kontrol aktivitelerine göre, bu 2-aminotiazol türevlerinin
etkileştiği GR aktivitelerinde herhangi bir istatistiksel değişim
gözlemlenmemiştir   (p >  0,05, n = 3).

Anahtar Kelimeler

Glutatyon Redüktaz, 2-Aminotiazol, 4, 4’-(disulfanediylbis(methylene)) bis(thiazol-2-amine) dihyrochloride, 2-amino-4-(chloromethyl)thiazole hydrochloride

Determination of Glutathione Reductase Activity Changes Exposed to Some 2-Aminothiazole Derivatives

Öz

In this work, effects of concentrations ranging
from 0 to 500 mg/L of some 2-aminothiazole derivatives such as
4,4’-(disulfanediylbis(methylene))bis(thiazol-2-amine) dihyrochloride (DMTA)
and 2-amino-4-(chloromethyl)thiazole hydrochloride (ACT) on glutathione
reductase from baker's yeast (Saccharomyces
cerevisiae) (GR) were investigated. With exposure of 25, 50, 100, 250 and
500 mg/L concentrations, % GR activity changes were calculated as -5.29 ; -3.85
; -2.40 ; -6.73 and -10.58 in DMTA applications, while these changes were
calculated as +0.98 ; 0.00 ; -0.49 ; -2.45 and 0.00  in ACT applications, respectively. This work
indicated that there was a slight decrease in GR activity with the increase of
DMTA concentrations and there was no significant change in GR activity with the
increase of ACT concentrations.  But
according to control activities, no statitistical changes were observed in GR
activities with exposure of these 2-aminothiazole derivatives (p > 0.05,
n=3).

Anahtar Kelimeler

Glutathione Reductase, 2-Aminothiazole, 2-amino-4-(chloromethyl)thiazole hydrochloride, 4, 4’-(disulfanediylbis(methylene)) bis(thiazol-2-amine) dihyrochloride

Kaynakça

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