Year 2019, Volume 40, Issue 1, Pages 150 - 157 2019-03-22

Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines
Baicalein’in Farklı Meme Kanseri Hücre hatlarındaki Kinetik Etkilerinin Değerlendirilmesi

Mehmet Rıfkı TOPÇUL [1]

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The aim of this study was to compare the antiproliferative effects of baicalein which is a flavonoide on MCF-7 and MDA-MB-231 cell line. The experiments were carried out with the evaluation of the parameters. Including cell viability, cell index, mitotic index, labelling index and apoptotic index. With the cell viability test, IC50 concentrations of baicalein for MCF-7 and MDA-MB-231 cells were determined as 10 µM and 30 µM, respectively and these concentrations were used in all experiments. The results showed that the IC50 concentrations decreased the values of cell viability, cell index, mitotic index and labelling index and increased the apoptotic index value for both cell types. These decreases and increases are statistically significant (p<0.01). In addition, curves of the graphs obtained from the xCelligence Real-Time Cell Analysis System showed that baicalein has cytoskeletal effects on MCF-7 cells and cytostatic effects on MDA-MB-231 cells. The results obtained from this study suggest that baicalein can be used in patients with luminal A and triple negative breast cancer in the clinic by supporting the future studies due to its antiproliferative effects on MCF-7 and MDA-MB-231 cells.

Bu çalışmada, bir flavonoid olan baicalein’in MCF-7 ve MDA-MB-231 hücre hatları üzerinde meydana getireceği antiproliferatif etkilerin karşılaştırılması amaçlanmıştır. Hücre canlılığı, hücre indeksi, mitotik indeks, işaretlenme indeksi ve apoptotik indeks gibi hücre kinetiği parametrelerinin değerlendirilmesi ile deneyler gerçekleştirilmiştir.

Yapılan hücre canlılığı testi ile baicalein’in MCF-7 ve MDA-MB-231 hücreleri için IC50 konsantrasyonları sırasıyla 10 µM ve 30 µM olarak belirlenmiş ve tüm deneylerde bu konsantrasyonlar kullanılmıştır. Elde edilen sonuçlar kullanılan IC50 konsantrasyonların her hücre tipi için de hücre canlılığı, hücre indeksi, mitotik indeks ve işaretlenme indeksi değerlerini azaltırken apoptotik indeks değerini yükselttiğini göstermiştir. Bu azalma ve artışlar istatistiksel olarak anlamlıdır (p<0.01). Ayrıca xCelligence Gerçek Zamanlı Hücre Analiz Sisteminden elde edilen grafiklere ait eğriler, baicalein’in MCF-7 hücrelerinde sitoskeletal etkiler meydana getirirken MDA-MB-231 hücrelerinde sitostatik etki meydana getirdiğini göstermiştir. Bu çalışmadan elde edilen sonuçlar baicalein’in MCF-7 ve MDA-MB-231 hücreleri üzerine antiproliferatif etkilerinden dolayı ileride yapılacak çalışmalarla desteklenerek, klinikte luminal A ve üçlü negatif meme kanseri hastaları için de kullanılabileceğini düşündürmektedir.

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Primary Language en
Subjects Basic Sciences
Journal Section Natural Sciences
Authors

Orcid: 0000-0002-9685-9404
Author: Mehmet Rıfkı TOPÇUL (Primary Author)
Institution: FACULTY OF SCIENCE
Country: Turkey


Dates

Publication Date: March 22, 2019

Bibtex @research article { csj511141, journal = {Cumhuriyet Science Journal}, issn = {2587-2680}, eissn = {2587-246X}, address = {Cumhuriyet University}, year = {2019}, volume = {40}, pages = {150 - 157}, doi = {10.17776/csj.511141}, title = {Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines}, key = {cite}, author = {TOPÇUL, Mehmet Rıfkı} }
APA TOPÇUL, M . (2019). Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines. Cumhuriyet Science Journal, 40 (1), 150-157. DOI: 10.17776/csj.511141
MLA TOPÇUL, M . "Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines". Cumhuriyet Science Journal 40 (2019): 150-157 <http://dergipark.org.tr/csj/issue/43798/511141>
Chicago TOPÇUL, M . "Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines". Cumhuriyet Science Journal 40 (2019): 150-157
RIS TY - JOUR T1 - Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines AU - Mehmet Rıfkı TOPÇUL Y1 - 2019 PY - 2019 N1 - doi: 10.17776/csj.511141 DO - 10.17776/csj.511141 T2 - Cumhuriyet Science Journal JF - Journal JO - JOR SP - 150 EP - 157 VL - 40 IS - 1 SN - 2587-2680-2587-246X M3 - doi: 10.17776/csj.511141 UR - https://doi.org/10.17776/csj.511141 Y2 - 2019 ER -
EndNote %0 Cumhuriyet Science Journal Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines %A Mehmet Rıfkı TOPÇUL %T Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines %D 2019 %J Cumhuriyet Science Journal %P 2587-2680-2587-246X %V 40 %N 1 %R doi: 10.17776/csj.511141 %U 10.17776/csj.511141
ISNAD TOPÇUL, Mehmet Rıfkı . "Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines". Cumhuriyet Science Journal 40 / 1 (March 2019): 150-157. https://doi.org/10.17776/csj.511141
AMA TOPÇUL M . Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines. CSJ. 2019; 40(1): 150-157.
Vancouver TOPÇUL M . Evaluation of Kinetic Effects of Baicalein in Different Breast Cancer Cell Lines. Cumhuriyet Science Journal. 2019; 40(1): 157-150.