Year 2019, Volume 7, Issue 3, Pages 2057 - 2073 2019-07-31

Soğuk Aktif ve Alkali Subtilisin Benzeri Proteaz Enziminin Bacillus sp. strain EL-GU1’ den İzolasyonu, Saflaştırılması ve Karekterizasyonu
Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1

Elif Guduk [1] , Gulhan Yasar [2] , Unzile Guven Gulhan [3] , Fatih Aktaş [4]

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One of the important hydrolytic enzymes are proteases that slice peptide bonds between amino acid residues. Proteases have various industrial applications including detergent, food, pharmaceutical, leather and diagnostic reagent industries. Among them, the most commercialized enzymes are alkaline proteases in the industry. Due to their potential applications in the detergent industry as cleaning additives, they are of particular interest. In this study, a novel protease from Bacillus sp. strain EL-GU1 was reported showing highest activity at pH 6 and 20°C. The novel protease was purified by using ammonium sulfate precipitation and identified by 16S rDNA sequencing. Highest activity was observed as 3300 µmol/min-1mg-1 when casein used as a substrate. Kinetic parameters of the enzyme were determined; KM, Vmax, kcat and catalytic efficiency values were calculated as 1.4 mM, 1 mM/s, 2.10-7 s-1, 0.14 10-7 s-1M-1, respectively. These results indicated that the novel cold active protease from Bacillus sp. strain EL- GU1 can be a good candidate for the detergent industry.

 

Proteases are hydrolytic enzymes that slice peptide bonds between amino acid residues and these enzymes have various industrial applications including detergent, food, pharmaceutical, leather and diagnostic reagent industries. Among them, alkaline proteases, the most commercialized enzymes in the industry, are of particular interest due to their potential applications in the detergent industry as cleaning additives. In this study, a novel alkaline protease from Bacillus sp. strain EL-GU1 was reported showing highest activity at pH 6 and 25°C. The novel protease was purified by using ammonium sulfate precipitation and identified by 16S rDNA sequencing. Highest activity was observed as 3300 µmol/min-1mg-1 when casein used as a substrate. Kinetic parameters of the enzyme were determined; KM, Vmax, kcat and catalytic efficiency values were calculated as 1.4 mM, 1 mM/s, 2.10-7 s-1, 0.14 10-7 s-1M-1, respectively. These results indicated that the novel cold active protease from Bacillus sp. strain EL- GU1 can be a good candidate for the detergent industry

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Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-9724-0566
Author: Elif Guduk
Country: United States


Orcid: 0000-0001-9759-7410
Author: Gulhan Yasar
Institution: ISTANBUL UNIVERSITY
Country: Turkey


Orcid: 0000-0002-5608-0447
Author: Unzile Guven Gulhan
Institution: GEBZE TECHNICAL UNIVERSITY
Country: Turkey


Orcid: 0000-0002-2031-298X
Author: Fatih Aktaş (Primary Author)
Institution: DUZCE UNIVERSITY
Country: Turkey


Dates

Publication Date: July 31, 2019

Bibtex @research article { dubited537340, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {Duzce University}, year = {2019}, volume = {7}, pages = {2057 - 2073}, doi = {10.29130/dubited.537340}, title = {Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1}, key = {cite}, author = {Guduk, Elif and Yasar, Gulhan and Guven Gulhan, Unzile and Aktaş, Fatih} }
APA Guduk, E , Yasar, G , Guven Gulhan, U , Aktaş, F . (2019). Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 7 (3), 2057-2073. DOI: 10.29130/dubited.537340
MLA Guduk, E , Yasar, G , Guven Gulhan, U , Aktaş, F . "Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 2057-2073 <http://dergipark.org.tr/dubited/issue/46290/537340>
Chicago Guduk, E , Yasar, G , Guven Gulhan, U , Aktaş, F . "Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 2057-2073
RIS TY - JOUR T1 - Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1 AU - Elif Guduk , Gulhan Yasar , Unzile Guven Gulhan , Fatih Aktaş Y1 - 2019 PY - 2019 N1 - doi: 10.29130/dubited.537340 DO - 10.29130/dubited.537340 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 2057 EP - 2073 VL - 7 IS - 3 SN - -2148-2446 M3 - doi: 10.29130/dubited.537340 UR - https://doi.org/10.29130/dubited.537340 Y2 - 2019 ER -
EndNote %0 Duzce University Journal of Science and Technology Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1 %A Elif Guduk , Gulhan Yasar , Unzile Guven Gulhan , Fatih Aktaş %T Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1 %D 2019 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 7 %N 3 %R doi: 10.29130/dubited.537340 %U 10.29130/dubited.537340
ISNAD Guduk, Elif , Yasar, Gulhan , Guven Gulhan, Unzile , Aktaş, Fatih . "Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 / 3 (July 2019): 2057-2073. https://doi.org/10.29130/dubited.537340
AMA Guduk E , Yasar G , Guven Gulhan U , Aktaş F . Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. DUBİTED. 2019; 7(3): 2057-2073.
Vancouver Guduk E , Yasar G , Guven Gulhan U , Aktaş F . Isolation, Purification and Characterization of new cold active subtilisin-like protease from Bacillus sp. strain EL-GU1. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2019; 7(3): 2073-2057.