Chemical Composition of Lamium purpureum L. and Determination of Anticancer Activity of Its Essential Oil on Melanoma

Yıl 2019, Cilt: 7 Sayı: 3, 1755 - 1763, 31.07.2019

Ayşegül Akkoyunlu Gorkem Dulger

https://doi.org/10.29130/dubited.553793

Öz

The current study aimed to evaluate Lamium purpureum L. in terms of its chemical composition and the potential of its essential oil for anticancer activity. The profiling analysis of its chemical composition was carried out via GC-MS. For this purpose, the ethanol extract was subjected to GC-MS analysis to determine chemical compounds. To obtain essential oil, steam distillation of the whole plant (aerial, leaf, stem) was carried out in a Clevenger apparatus. The anticancer activitiy was determined via MTT assay. We observed that the maximum cell death was 14% at 50 μg/mL concentration. As a result of GC-MS analysis, palmitic acid, 7-tetradecenal, octadecadienoic acid, acetic acid, ethyl 9,12,15-octadecatrienoate, hexatriacontane, stearate and benzyl benzoate were identified as major components. We determined that the essential oil of L. purpureum L. had essential compounds which showed cytotoxic activities.

Anahtar Kelimeler

GC-MS analysis, Anticancer activity, MTT, Essential oil, Lamium purpureum L.

Lamium purpureum L.’nin Kimyasal Bileşimi ve Uçucu Yağının İnsan Melanom Kanseri Üzerine Antikanser Aktivitesinin Belirlenmesi

Öz

Bu çalışma Lamium purpureum L.'yi kimyasal bileşimi ve antikanser aktivitesi için uçucu yağ potansiyeli açısından değerlendirmeyi amaçlamıştır. Kimyasal bileşiminin profil analizi GC-MS ile yapılmıştır. Bu amaçla, etanol ekstresi kimyasal bileşiklerin belirlenmesi için GC-MS analizine tabi tutuldu. Esansiyel yağ elde etmek için, tüm bitkinin (hava, yaprak, gövde) buharla damıtılması bir Clevenger aparatında gerçekleştirildi. Antikanser aktivitesi MTT testi ile belirlendi. 50 μg/mL konsantrasyonunda maksimum hücre ölümünün% 14 olduğunu gözlemledik. GC-MS analizi sonucunda palmitik asit, 7-tetradekenal, oktadecadienoik asit, asetik asit, etil 9,12,15-oktadekatrienoat, heksatriakontan, stearat ve benzil benzoat ana bileşenler olarak tanımlandı. L. purpureum L.'ın uçucu yağının sitotoksik aktivite gösteren temel bileşiklere sahip olduğunu belirledik.

Anahtar Kelimeler

GC-MS analizi, Antikanser aktivite, MTT, Esansiyel yağ, Lamium purpureum L.

Kaynakça

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