Year 2019, Volume 7, Issue 3, Pages 1806 - 1812 2019-07-31

Elektrokimyasal Biyosensörlerin Geliştirilmesinde Alkanetiol Kendiliğinden Oluşan Tek Katmanlar ile Peptid Nükleik Asit (PNA) Probu
Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors

Zehra Banu Bahsi Oral [1]

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Peptid Nükleik Asit (PNA) içeren alkanetiol kendiliğinden oluşan tek katmanlar (SAM), elektrokimyasal biyosensörlerde prob olarak önerilmiştir. Bu makale, PNA probunun optimizasyonunu iki farklı SAM konsantrasyonu, immobilizasyon sıcaklığı ve iki aşamalı immobilizasyon prosesi kullanarak araştırmıştır. Alkanetiol kendiliğinden oluşan tek katmanlar ile PNA prob immobilizasyonu ve cDNA (tamamlayıcı DNA) hibridizasyonu AC voltametre metodu ile ölçülmüştür. Yüksek iyonik kuvvet konsantrasyonunda, cDNA hibridizasyonundan sonra formal potansiyelde küçük negatif kayma (-17 mV) meydana gelmiştir

Alkanethiol self-assembled monolayers (SAMs) with Peptide Nucleic Acid (PNA) were proposed as a novel probe design in electrochemical biosensors. This paper investigates the optimization of PNA probe by using two different concentrations of SAMs, immobilization temperature, and two-stage immobilization process. Alkanethiol self-assembled monolayers with PNA probe immobilization and cDNA (complementary DNA) hybridization were measured via AC voltammetry method. The small negative shift in formal potential (-17 mV) was occurred after cDNA hybridization at high ionic strength concentration.

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Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0001-5513-0847
Author: Zehra Banu Bahsi Oral (Primary Author)
Institution: GEBZE TEKNİK ÜNİVERSİTESİ, BİYOTEKNOLOJİ ENSTİTÜSÜ
Country: Turkey


Dates

Publication Date: July 31, 2019

Bibtex @research article { dubited558155, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {Duzce University}, year = {2019}, volume = {7}, pages = {1806 - 1812}, doi = {10.29130/dubited.558155}, title = {Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors}, key = {cite}, author = {Bahsi Oral, Zehra Banu} }
APA Bahsi Oral, Z . (2019). Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 7 (3), 1806-1812. DOI: 10.29130/dubited.558155
MLA Bahsi Oral, Z . "Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1806-1812 <http://dergipark.org.tr/dubited/issue/46290/558155>
Chicago Bahsi Oral, Z . "Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1806-1812
RIS TY - JOUR T1 - Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors AU - Zehra Banu Bahsi Oral Y1 - 2019 PY - 2019 N1 - doi: 10.29130/dubited.558155 DO - 10.29130/dubited.558155 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 1806 EP - 1812 VL - 7 IS - 3 SN - -2148-2446 M3 - doi: 10.29130/dubited.558155 UR - https://doi.org/10.29130/dubited.558155 Y2 - 2019 ER -
EndNote %0 Duzce University Journal of Science and Technology Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors %A Zehra Banu Bahsi Oral %T Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors %D 2019 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 7 %N 3 %R doi: 10.29130/dubited.558155 %U 10.29130/dubited.558155
ISNAD Bahsi Oral, Zehra Banu . "Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 / 3 (July 2019): 1806-1812. https://doi.org/10.29130/dubited.558155
AMA Bahsi Oral Z . Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors. DUBİTED. 2019; 7(3): 1806-1812.
Vancouver Bahsi Oral Z . Self -Assembled Monolayers with Peptide Nucleic Acid (PNA) Probe to Develop Electrochemical Biosensors. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2019; 7(3): 1812-1806.