Year 2019, Volume 7, Issue 3, Pages 1879 - 1891 2019-07-31

The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress
L-Triptofan ve Melatonin’in Tuz Stresi Altında Bazı Serin Iklim Sebze Türlerinin Tohum Çimlenmesine Etkileri

Fatih HANCI [1]

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This study was carried out with the objective to evaluate the effects of different concentrations of L-Tryptophan and Melatonin pretreatment on germination behavior of onion, leek, black carrot, and radish seeds exposed to saline stress. Seeds of samples were soaked for 24 hours in distilled water, 50,100, 150 ppm L-tryptophan, and 1.5, 3, and 4.5 µM melatonin solutions at 20° C in darkness. Seeds were placed in Petri dishes and watered to moisten the filter paper with different concentrations of NaCl (0, 150, 300 or 450 mM). The experiment was planned in a completely randomized design with four replications. Maximum germination percentage, germination index, time to complete 50% germination and mean germination time was measured at the end of the study. The results indicated that the effect of pre-treatment was significant on all studied traits in all species except for mean germination time for black carrot seeds. In addition, all germination parameters of seeds were delayed and decreased by salinity increasing from 0 mM to 450 mM NaCl. Overall, all doses of melatonin increased the maximum germination ratio and germination index values slightly under 300 mM NaCl stress conditions. In general, results which obtained from all doses of melatonin were close to each other, whereas increasing doses of L-tryptophan caused a negative effect in some cases. 

Bu çalışma, farklı konsantrasyonlarda L-Triptofan ve Melatonin ön uygulamalarının, tuz stresine maruz bırakılan soğan, pırasa, siyah havuç ve turp tohumlarının çimlenme özellikleri üzerine etkilerini değerlendirmek amacıyla gerçekleştirilmiştir. Örneklere ait tohumlar, 24 saat boyunca, distile su, 50, 100, 150 ppm L-triptofan;  1.5, 3 ve 4.5 µM Melatonin çözeltilerinde karanlıkta ve 20 ° C' de çözeltilerinde bekletilmiştir. Tohumlar petri kaplarına yerleştirilmiş ve filtre kağıtları farklı NaCl konsantrasyonlarında (0, 150, 300 veya 450 mM) su ile nemlendirilmiştir. Deneme, dört tekerrürlü  tesadüf parselleri şeklinde planlanmıştır. Çalışmanın sonunda maksimum çimlenme yüzdesi, çimlenme indeksi, % 50 çimlenmeyi tamamlama süresi ve ortalama çimlenme süresi değerleri ölçülmüştür. Sonuçlar, ön uygulamaların etkilerinin, siyah havuç tohumlarının ortalama çimlenme süresi hariç, tüm türlerde ve değerlendirilen tüm özellikler üzerinde önemli etkilere neden olduğunu göstermiştir. Buna ek olarak, tuzluluk derecesinin 0 mM' den 450 mM' ye yükselmesi, tohumların tüm çimlenme parametrelerini azaltmış ve geciktirmiştir. Genel olarak, tüm melatonin dozları, 300 mM NaCl stres koşulları altında çimlenme oranını ve çimlenme endeksini bir miktar artırmıştır. Genel olarak, tüm melatonin dozlarından elde edilen sonuçlar birbirine yakın olmasına rağmen, artan L-Triptofan dozları bazı durumlarda olumsuz etkilere neden olmuştur.

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Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-2015-0351
Author: Fatih HANCI (Primary Author)
Institution: ERCIYES UNIVERSITY
Country: Turkey


Supporting Institution Erciyes Üniversitesi, Ziraat Fakültesi
Project Number FLO-2018-8572
Thanks This work is supported by Research Fund of the Erciyes University. Project Number: FLO-2018-8572.
Dates

Publication Date: July 31, 2019

Bibtex @research article { dubited567005, journal = {Düzce Üniversitesi Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2446}, address = {Duzce University}, year = {2019}, volume = {7}, pages = {1879 - 1891}, doi = {10.29130/dubited.567005}, title = {The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress}, key = {cite}, author = {HANCI, Fatih} }
APA HANCI, F . (2019). The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress. Düzce Üniversitesi Bilim ve Teknoloji Dergisi, 7 (3), 1879-1891. DOI: 10.29130/dubited.567005
MLA HANCI, F . "The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1879-1891 <http://dergipark.org.tr/dubited/issue/46290/567005>
Chicago HANCI, F . "The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 (2019): 1879-1891
RIS TY - JOUR T1 - The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress AU - Fatih HANCI Y1 - 2019 PY - 2019 N1 - doi: 10.29130/dubited.567005 DO - 10.29130/dubited.567005 T2 - Düzce Üniversitesi Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 1879 EP - 1891 VL - 7 IS - 3 SN - -2148-2446 M3 - doi: 10.29130/dubited.567005 UR - https://doi.org/10.29130/dubited.567005 Y2 - 2019 ER -
EndNote %0 Duzce University Journal of Science and Technology The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress %A Fatih HANCI %T The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress %D 2019 %J Düzce Üniversitesi Bilim ve Teknoloji Dergisi %P -2148-2446 %V 7 %N 3 %R doi: 10.29130/dubited.567005 %U 10.29130/dubited.567005
ISNAD HANCI, Fatih . "The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress". Düzce Üniversitesi Bilim ve Teknoloji Dergisi 7 / 3 (July 2019): 1879-1891. https://doi.org/10.29130/dubited.567005
AMA HANCI F . The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress. DUBİTED. 2019; 7(3): 1879-1891.
Vancouver HANCI F . The Effect of L-Tryptophan and Melatonin on Seed Germination of Some Cool Season Vegetable Species Under Salinity Stress. Düzce Üniversitesi Bilim ve Teknoloji Dergisi. 2019; 7(3): 1891-1879.