Year 2018, Volume , Issue 14, Pages 388 - 392 2018-12-31

Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels

Murat Uygun [1] , Melis Bayraktaroğlu [2] , Gülşen Güven [3] , Sinem Evli [4] , Fatma Akpınar [5] , Deniz Aktaş Uygun [6]

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In this presented work, poly(2-hydroxyethyl methacrylate-glycidyl methacrylate) [poly(HEMA-GMA)] cryogels were prepared by using the free radical cryopolymerization technique. These cryogels were then modified with peroxidase enzyme by covalent bonding. These peroxidase immobilized cryogels were successfully used for the removing of phenol and bisphenol A and decolorization of a model dye molecule.

Peroxidase, cryogel, phenol removal, decolorization
  • [1].Bayramoğlu, G., Arıca, M.Y. (2008) Enzymatic removal o phenol and p-chlorophenol in enzyme reactor: Horseradish peroxidase immobilized on magnetic beads. Journal of Hazardous Materials, 156, 148-155.
  • [2].De Queiroz, A. A. A., Vargas, R. R., Higa, O. Z., Barrak, É. R., Bechara, E. J., Wlasdislaw, B., & Marzorati, L. (1999). Graft copolymers with immobilized peroxidase for organic synthesis. Radiation Physics and Chemistry, 55(3), 345-352.
  • [3].H.B. Dunford, Heme Peroxidases, John Wiley & Sons, Inc., New York, 1999
  • [4].Nicell, J. A., Bewtra, J. K., Biswas, N., & Taylor, E. (1993). Reactor development for peroxidase catalyzed polymerization and precipitation of phenols from wastewater. Water Research, 27(11), 1629-1639.
  • [5].Jeng, F. Y., & Lin, S. C. (2006). Characterization and application of PEGylated horseradish peroxidase for the synthesis of poly (2-naphthol). Process Biochemistry, 41(7), 1566-1573.
  • [6].Buchanan, I. D., & Nicell, J. A. (1997). Model development for horseradish peroxidase catalyzed removal of aqueous phenol. Biotechnology and bioengineering, 54(3), 251-261.
  • [7].Choi, Y. J., Chae, H. J., & Kim, E. Y. (1999). Steady-state oxidation model by horseradish peroxidase for the estimation of the non-inactivation zone in the enzymatic removal of pentachlorophenol. Journal of bioscience and bioengineering, 88(4), 368-373.
  • [8].Wu, Y., Taylor, K. E., Biswas, N., & Bewtra, J. K. (1999). Kinetic model‐aided reactor design for peroxidase‐catalyzed removal of phenol in the presence of polyethylene glycol. Journal of Chemical Technology & Biotechnology: International Research in Process, Environmental & Clean Technology, 74(6), 519-526.
  • [9].Van Haandel, M. J., Saraber, F. C., Boersma, M. G., Laane, C., Fleming, Y., Weenen, H., & Rietjens, I. M. (2000). Characterization of different commercial soybean peroxidase preparations and use of the enzyme for N-demethylation of methyl N-methylanthranilate to produce the food flavor methylanthranilate. Journal of agricultural and food chemistry, 48(5), 1949-1954.
  • [10].Dembitsky, V. M. (2003). Oxidation, epoxidation and sulfoxidation reactions catalysed by haloperoxidases. Tetrahedron, 26(59), 4701-4720.
  • [11].Wagner, M., & Nicell, J. A. (2005). Evaluation of horseradish peroxidase for the treatment of estrogenic alkylphenols. Water Quality Research Journal, 40(2), 145-154.
  • [12].Vojinović, V., Carvalho, R. H., Lemos, F., Cabral, J. M. S., Fonseca, L. P., & Ferreira, B. S. (2007). Kinetics of soluble and immobilized horseradish peroxidase-mediated oxidation of phenolic compounds. Biochemical engineering journal, 35(2), 126-135.
  • [13].Zai, R., Zhang, B., Wan, Y., Li, C., Wang, J., & Liu, J. (2013). Chitosan-halloysite hyride-nanotubes: Horseradish peroxidase immobilization and application in phenol removal. Chemical Engineering Journal, 214, 304-309.
  • [14].Xu, R., Chi, C., Li, F., Zhang, B. (2013). Immobilization of horseradish peroxidase on electrospun microfibrous membranes for biodegradation and adsorption of bisphenol A. Bioresource Technology, 149, 111-116.
  • [15].Qiu, H., Lu, L., Huang, X., Zhang, Z., Qu, Y. (2010). Immobilization of horseradish peroxidase on nanoporous copper and its potential applications. Bioresource Technology, 101, 9415-9420.
  • [16].Mohamed, S. A., Aly, A. S., Mohamed, T. M., Salah, H. A. (2008). Immobilization of horseradish peroxidase on nonwoven polyester fabric coated with chitosan. Applied Biochemistry and Biotechnology, 144, 169-179.
  • [17].Chouyyok, W., Panpranot, J., Thanachayanant, C., Prichanont, S. (2009). Effects of pH and pore characters of mesoporous silicas on horseradish peroxidase immobilization. Journal of Molecular Catalysis B: Enzymatic, 56, 246-252.
  • [18].Uygun, M. (2014). Dye-attached cryogels for reversible alcohol dehydrogenase immobilization. Journal of Chromatography B, 959, 42-48.
  • [19].Follmer, C., Wassermann, G. E., & Carlini, C. R. (2004). Separation of jack bean (Canavalia ensiformis) urease isoforms by immobilized metal affinity chromatography and characterization of insecticidal properties unrelated to ureolytic activity. Plant science, 167(2), 241-246.
  • [20].Dainiak, M. B., Galaev, I. Y., & Mattiasson, B. (2006). Affinity cryogel monoliths for screening for optimal separation conditions and chromatographic separation of cells. Journal of Chromatography A, 1123(2), 145-150.
  • [21].Galaev, I. Y., & Mattiasson, B. (2008). Affinity Processing of Cell-Containing Feeds Using Monolithic Macroporous Hydrogels, Cryogels. In Affinity Chromatography (pp. 247-256). Humana Press.
  • [22].Akduman, B., Uygun, M., Uygun, D. A., Akgöl, S., & Denizli, A. (2013). Purification of yeast alcohol dehydrogenase by using immobilized metal affinity cryogels. Materials Science and Engineering: C, 33(8), 4842-4848.
  • [23].Wong, Y., & Yu, J. (1999). Laccase-catalyzed decolorization of synthetic dyes. Water research, 33(16), 3512-3520.
  • [24].Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical biochemistry, 72(1-2), 248-254.
  • [25].Trinder, P. (1969). Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Annals of clinical Biochemistry, 6(1), 24-27.
  • [26].Gomez, J. L., Bodalo, A., Gomez, E., Bastida, J., Hidalgo, A. M., & Gomez, M. (2006). Immobilization of peroxidases on glass beads: an improved alternative for phenol removal. Enzyme and microbial technology, 39(5), 1016-1022.
  • [27].Uygun, M., Akduman, B., Akgöl, S., & Denizli, A. (2013). A new metal-chelated cryogel for reversible immobilization of urease. Applied biochemistry and biotechnology, 170(8), 1815-1826.
  • [28].Lai, Y. C., & Lin, S. C. (2005). Application of immobilized horseradish peroxidase for the removal of p-chlorophenol from aqueous solution. Process Biochemistry, 40(3-4), 1167-1174.
  • [29].Kalaiarasan, E., & Palvannan, T. (2014). Removal of phenols from acidic environment by horseradish peroxidase (HRP): aqueous thermostabilization of HRP by polysaccharide additives. Journal of the Taiwan Institute of Chemical Engineers, 45(2), 625-634.
  • [30].Kunamneni, A., Ghazi, I., Camarero, S., Ballesteros, A., Plou, F. J., & Alcalde, M. (2008). Decolorization of synthetic dyes by laccase immobilized on epoxy-activated carriers. Process Biochemistry, 43(2), 169-178.
  • [31].Murugesan, K., Arulmani, M., Nam, I. H., Kim, Y. M., Chang, Y. S., & Kalaichelvan, P. T. (2006). Purification and characterization of laccase produced by a white rot fungus Pleurotus sajor-caju under submerged culture condition and its potential in decolorization of azo dyes. Applied Microbiology and Biotechnology, 72(5), 939-946.
  • [32].Murugesan, K., Nam, I. H., Kim, Y. M., & Chang, Y. S. (2007). Decolorization of reactive dyes by a thermostable laccase produced by Ganoderma lucidum in solid state culture. Enzyme and Microbial Technology, 40(7), 1662-1672.
  • [33].Peralta-Zamora, P., Pereira, C. M., Tiburtius, E. R., Moraes, S. G., Rosa, M. A., Minussi, R. C., & Durán, N. (2003). Decolorization of reactive dyes by immobilized laccase. Applied Catalysis B: Environmental, 42(2), 131-144.
  • [34].Claus, H., Faber, G., & König, H. (2002). Redox-mediated decolorization of synthetic dyes by fungal laccases. Applied Microbiology and Biotechnology, 59(6), 672-678.
Primary Language en
Subjects Engineering
Journal Section Makaleler
Authors

Orcid: 0000-0002-5879-8022
Author: Murat Uygun (Primary Author)
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
Country: Turkey


Orcid: 0000-0000-0000-0000
Author: Melis Bayraktaroğlu
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ
Country: Turkey


Orcid: 0000-0000-0000-0000
Author: Gülşen Güven
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
Country: Turkey


Orcid: 0000-0000-0000-0000
Author: Sinem Evli
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ
Country: Turkey


Orcid: 0000-0000-0000-0000
Author: Fatma Akpınar
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN BİLİMLERİ ENSTİTÜSÜ
Country: Turkey


Orcid: 0000-0002-5633-2704
Author: Deniz Aktaş Uygun
Institution: ADNAN MENDERES ÜNİVERSİTESİ, FEN-EDEBİYAT FAKÜLTESİ
Country: Turkey


Dates

Publication Date: December 31, 2018

Bibtex @research article { ejosat490358, journal = {Avrupa Bilim ve Teknoloji Dergisi}, issn = {}, eissn = {2148-2683}, address = {Osman SAĞDIÇ}, year = {2018}, volume = {}, pages = {388 - 392}, doi = {10.31590/ejosat.490358}, title = {Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels}, key = {cite}, author = {Uygun, Murat and Bayraktaroğlu, Melis and Güven, Gülşen and Evli, Sinem and Akpınar, Fatma and Aktaş Uygun, Deniz} }
APA Uygun, M , Bayraktaroğlu, M , Güven, G , Evli, S , Akpınar, F , Aktaş Uygun, D . (2018). Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels. Avrupa Bilim ve Teknoloji Dergisi, (14), 388-392. DOI: 10.31590/ejosat.490358
MLA Uygun, M , Bayraktaroğlu, M , Güven, G , Evli, S , Akpınar, F , Aktaş Uygun, D . "Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels". Avrupa Bilim ve Teknoloji Dergisi (2018): 388-392 <http://dergipark.org.tr/ejosat/issue/40225/490358>
Chicago Uygun, M , Bayraktaroğlu, M , Güven, G , Evli, S , Akpınar, F , Aktaş Uygun, D . "Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels". Avrupa Bilim ve Teknoloji Dergisi (2018): 388-392
RIS TY - JOUR T1 - Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels AU - Murat Uygun , Melis Bayraktaroğlu , Gülşen Güven , Sinem Evli , Fatma Akpınar , Deniz Aktaş Uygun Y1 - 2018 PY - 2018 N1 - doi: 10.31590/ejosat.490358 DO - 10.31590/ejosat.490358 T2 - Avrupa Bilim ve Teknoloji Dergisi JF - Journal JO - JOR SP - 388 EP - 392 VL - IS - 14 SN - -2148-2683 M3 - doi: 10.31590/ejosat.490358 UR - https://doi.org/10.31590/ejosat.490358 Y2 - 2018 ER -
EndNote %0 European Journal of Science and Technology Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels %A Murat Uygun , Melis Bayraktaroğlu , Gülşen Güven , Sinem Evli , Fatma Akpınar , Deniz Aktaş Uygun %T Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels %D 2018 %J Avrupa Bilim ve Teknoloji Dergisi %P -2148-2683 %V %N 14 %R doi: 10.31590/ejosat.490358 %U 10.31590/ejosat.490358
ISNAD Uygun, Murat , Bayraktaroğlu, Melis , Güven, Gülşen , Evli, Sinem , Akpınar, Fatma , Aktaş Uygun, Deniz . "Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels". Avrupa Bilim ve Teknoloji Dergisi / 14 (December 2019): 388-392. https://doi.org/10.31590/ejosat.490358
AMA Uygun M , Bayraktaroğlu M , Güven G , Evli S , Akpınar F , Aktaş Uygun D . Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels. Avrupa Bilim ve Teknoloji Dergisi. 2018; (14): 388-392.
Vancouver Uygun M , Bayraktaroğlu M , Güven G , Evli S , Akpınar F , Aktaş Uygun D . Environmental Applications of Immobilized Peroxidase onto Epoxy Bearing Cryogels. Avrupa Bilim ve Teknoloji Dergisi. 2018; (14): 392-388.