The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle

Muhammed Furkan Çiftçi; Maide Gölbaşi; Ömer Faruk Yeşilkaya; Ayşe Sari; Sakine Ülküm Çizmeci; Dursun Ali Dinç

doi:10.30607/kvj.1548550

EN TR

The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle

Abstract

The aim of this study was to determine the effect of antral follicle number on oocyte yield and IVEP in Holstein breed donors. The study groups were constituted following ultrasonographic examinations conducted on 23 Holstein heifers at one-week intervals. The Low-AFC group (n = 8) comprised animals with an average antral follicle counts in the ovary of ≤ 15, as determined by ultrasonographic examination. The High-AFC group (n = 9) comprised animals with an average antral follicle number of ≥30. Oocytes were collected from animals in both groups four times, one week apart, any day of the estrous cycle. The oocytes were classified according to their morphological characteristics and subsequently incorporated into the in vitro embryo production process. The total and viable oocyte numbers were found to be significantly greater in the High-AFC group than in the Low-AFC group (P < 0.01). The number of blastocysts obtained per session was also greater in the High-AFC group (P < 0.01). No statistically significant difference was observed between the blastocyst and viable oocyte rates in the two groups. Consequently, oocytes and blastocysts were obtained from Holsetin donors with high antral follicle counts in greater numbers. It was thought that the number of antral follicles in Holstein breed donors could be employed as a parameter in donor animal selection to increase the success of OPU/IVEP.

Keywords

Holstein Sığırlarda OPU/IVEP için Donör Seçiminde Antral Folikül Sayısının Rolü

Abstract

Bu çalışmada Holstein ırkı donörlerde antral folikül sayısının oosit verimine ve in vitro embriyo üretimine etkisinin belirlenmesi amaçlandı. Çalışma grupları 23 baş Holstein ırkı düveye 1 hafta arayla yapılan ultrasonografik muayeneler sonrasında oluşturuldu. Ultrasonografik muayene sonrasında ovaryumundaki ortalama antral folikül sayısı ≤ 15 olan hayvanlar Düşük-AFS grubuna (n=8) alındı. Ortalama antral folikül sayısı ≥ 30 olan hayvanlar ise Yüksek-AFS grubuna (n=9) alındı. Her iki gruptaki hayvanlardan östrüs siklusunun rastgele bir gününde birer hafta arayla 4 kez oosit toplandı. Toplanan oositler morfolojik özelliklerine göre sınıflandırılarak in vitro embriyo üretim sürecine alındı. Toplam ve in vitro embriyo üretimine uygun oosit sayısı Yüksek-AFS grubunda Düşük-AFS grubundan yüksek bulundu (P < 0.01). Seans başına elde edilen blastosist sayısı da Yüksek-AFS grubunda daha fazlaydı (P < 0.01). Blastosist oranı ve in vitro embriyo üretimine uygun oosit oranı iki grup arasında istatistiksel farklılık göstermedi. Sonuç olarak Holstein ırkı donörlerde yüksek antral folikül sayısına sahip hayvanlardan daha fazla sayıda oosit ve blastosist elde edildi. Holstein ırkı donörlerde antral folikül sayısının OPU/IVEP başarısını artırmak için donör hayvan seçiminde bir parametre olarak kullanılabileceği düşünüldü.

Keywords

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Details

Primary Language

English

Subjects

Veterinary Obstetrics and Gynecology

Journal Section

Research Article

Authors

Muhammed Furkan Çiftçi ^*
0000-0001-8333-6500
Türkiye

Maide Gölbaşi
0000-0002-0218-8313
Türkiye

Ömer Faruk Yeşilkaya
0000-0002-7721-2576
Türkiye

Ayşe Sari
0000-0003-4181-9509
Türkiye

Sakine Ülküm Çizmeci
0000-0003-2939-8019
Türkiye

Dursun Ali Dinç
0000-0002-9597-227X
Türkiye

Early Pub Date

March 13, 2025

Publication Date

March 28, 2025

Submission Date

September 11, 2024

Acceptance Date

December 27, 2024

Published in Issue

Year 2025 Volume: 18 Number: 1

DOI

https://doi.org/10.30607/kvj.1548550

IZ

https://izlik.org/JA59ZE67KW

Cite

RIS / Bibtex

APA

Çiftçi, M. F., Gölbaşi, M., Yeşilkaya, Ö. F., Sari, A., Çizmeci, S. Ü., & Dinç, D. A. (2025). The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle. Kocatepe Veterinary Journal, 18(1), 1-6. https://doi.org/10.30607/kvj.1548550

AMA

1.Çiftçi MF, Gölbaşi M, Yeşilkaya ÖF, Sari A, Çizmeci SÜ, Dinç DA. The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle. kvj. 2025;18(1):1-6. doi:10.30607/kvj.1548550

Chicago

Çiftçi, Muhammed Furkan, Maide Gölbaşi, Ömer Faruk Yeşilkaya, Ayşe Sari, Sakine Ülküm Çizmeci, and Dursun Ali Dinç. 2025. “The Role of Antral Follicle Count in OPU IVEP Donor Selection in Holstein Cattle”. Kocatepe Veterinary Journal 18 (1): 1-6. https://doi.org/10.30607/kvj.1548550.

EndNote

Çiftçi MF, Gölbaşi M, Yeşilkaya ÖF, Sari A, Çizmeci SÜ, Dinç DA (March 1, 2025) The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle. Kocatepe Veterinary Journal 18 1 1–6.

IEEE

[1]M. F. Çiftçi, M. Gölbaşi, Ö. F. Yeşilkaya, A. Sari, S. Ü. Çizmeci, and D. A. Dinç, “The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle”, kvj, vol. 18, no. 1, pp. 1–6, Mar. 2025, doi: 10.30607/kvj.1548550.

ISNAD

Çiftçi, Muhammed Furkan - Gölbaşi, Maide - Yeşilkaya, Ömer Faruk - Sari, Ayşe - Çizmeci, Sakine Ülküm - Dinç, Dursun Ali. “The Role of Antral Follicle Count in OPU IVEP Donor Selection in Holstein Cattle”. Kocatepe Veterinary Journal 18/1 (March 1, 2025): 1-6. https://doi.org/10.30607/kvj.1548550.

JAMA

1.Çiftçi MF, Gölbaşi M, Yeşilkaya ÖF, Sari A, Çizmeci SÜ, Dinç DA. The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle. kvj. 2025;18:1–6.

MLA

Çiftçi, Muhammed Furkan, et al. “The Role of Antral Follicle Count in OPU IVEP Donor Selection in Holstein Cattle”. Kocatepe Veterinary Journal, vol. 18, no. 1, Mar. 2025, pp. 1-6, doi:10.30607/kvj.1548550.

Vancouver

1.Muhammed Furkan Çiftçi, Maide Gölbaşi, Ömer Faruk Yeşilkaya, Ayşe Sari, Sakine Ülküm Çizmeci, Dursun Ali Dinç. The Role of Antral Follicle Count in OPU/IVEP Donor Selection in Holstein Cattle. kvj. 2025 Mar. 1;18(1):1-6. doi:10.30607/kvj.1548550