Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis

Yusuf Umut Batı; Mert Sezer; Mustafa Makav; Enes Akyüz; Mushap Kuru; Ali Haydar Kırmızıgül

doi:10.30607/kvj.1868070

EN TR

Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis

Abstract

Traumatic pericarditis most commonly develops during the course of traumatic reticuloperitonitis and is associated with an increased oxidative burden; however, the diagnostic contribution of oxidative biomarkers remains unclear. In this study, the oxidative stress profile and dynamic thiol–disulfide homeostasis were evaluated in cattle with traumatic pericarditis. Comparisons between the pericarditis and control groups were performed within the same sex, and sex-related differences were additionally investigated within the pericarditis subgroup. A total of 100 cattle were included in the pericarditis and control group comparisons. Serum malondialdehyde (MDA), reduced glutathione (GSH), total and native thiol (TT, NT), and disulfide (Ds) levels were measured spectrophotometrically, and Ds/TT, Ds/NT, and NT/TT ratios were calculated. Within the same sex, MDA levels were higher in the pericarditis group than in controls in both females (P<0.01) and males (P<0.05). GSH levels were lower in males with pericarditis (P<0.001), whereas no significant difference was detected in females (P>0.05). TT and NT decreased in the pericarditis group in both sexes (females: TT P<0.01, NT P<0.05; males: both P<0.0001). No significant change in Ds was observed in within-sex comparisons between pericarditis and control groups; however, in the sex comparison within the pericarditis subgroup, Ds levels were higher in females (P<0.01). In the traumatic pericarditis group, ratio biomarkers differed significantly between sexes: Ds/TT and Ds/NT were higher in females than in males, whereas the NT/TT ratio was higher in males (P<0.01). These findings suggest that pericarditis is accompanied by increased lipid peroxidation and a reduction in the antioxidant/thiol pool, and that impairment in thiol–disulfide balance exhibits a sex-specific pattern. The evaluated panels may provide complementary value to clinical assessment; however, prospective, multicenter validation and determination of threshold values are required for clinical implementation.

Keywords

Supporting Institution

There is no supporting organization.

Ethical Statement

This study was approved by the Kafkas University Animal Experiments Local Ethics Committee (KAU HADYEK) (Approval No: KAU HADYEK/2024-065).

Thanks

There are no individuals or organizations to thank within the scope of this study.

Travmatik Perikarditisli Sığırlarda Malondialdehit, Redükte Glutatyon ve Tiyol-Disülfid Homeostazının Değişimleri

Abstract

Travmatik perikarditis, çoğunlukla retikuloperitonitis travmatikayı izleyen süreçte gelişir ve artmış oksidatif yükle ilişkilidir; ancak tanıda oksidatif biyobelirteçlerin katkısı net değildir. Bu çalışmada, travmatik perikarditisli sığırlarda oksidatif stres profili ile dinamik tiyol–disülfid dengesi değerlendirilmiştir. Aynı cinsiyet içinde perikarditis ve kontrol grubu karşılaştırmaları yapılmış ve perikarditis alt grubunda dişi-erkek farkları ayrıca incelenmiştir. Perikarditis ve kontrol grupları karşılaştırmalarında toplam 100 sığır incelenmiştir. Serum malondialdehit (MDA), redükte glutatyon (GSH), toplam ve natif tiyol (TT, NT) ile disülfid (Ds) spektrofotometrik olarak ölçülmüş; Ds/TT, Ds/NT ve NT/TT oranları hesaplanmıştır. Aynı cinsiyet içinde, perikarditis varlığında MDA düzeyleri kontrol grubuna kıyasla her iki cinsiyette yüksek bulunmuştur (dişi P<0,01; erkek P<0,05). GSH düzeyleri perikarditisli erkeklerde düşük bulunmuştur (P<0,001), dişilerde ise anlamlı farklılık saptanmamıştır (P>0,05). TT ve NT her iki cinsiyette de perikarditisli grupta azalmıştır (dişide TT için P<0,01, NT için P<0,05; erkekte her ikisi için P<0,0001). Cinsiyet içi perikarditis ve kontrol grubu karşılaştırmalarında Ds’de anlamlı değişim saptanmamış; buna karşılık perikarditis alt grubunda dişi–erkek karşılaştırmasında Ds düzeyi dişilerde daha yüksek bulunmuştur (P<0,01). Perikarditis (TP) grubunda, cinsiyetler arası karşılaştırmada oran belirteçleri istatistiksel olarak anlamlı farklılık göstermiştir: Ds/TT ve Ds/NT değerleri dişilerde erkeklere kıyasla daha yüksek, NT/TT oranı ise erkeklerde daha yüksek bulunmuştur (P<0,01). Bulgular, perikarditisin artmış lipid peroksidasyonu ve antioksidan/tiyol havuzunda daralma ile seyrettiğini ve tiyol-disülfid dengesindeki bozulmanın cinsiyete özgü bir patern sergilediğini düşündürmektedir. Değerlendirilen paneller klinik değerlendirmeye tamamlayıcı katkı sağlayabilir; ancak klinik kullanım için prospektif, çok merkezli doğrulama ve eşik değerlerinin belirlenmesi gereklidir.

Keywords

Supporting Institution

Destekleyen herhangi bir kurum yoktur.

Ethical Statement

Bu çalışma, Kafkas Üniversitesi Hayvan Deneyleri Yerel Etik Kurulu (KAÜ HADYEK) tarafından onaylanmıştır (Karar No: KAÜ HADYEK/2024-065).

Thanks

Bu çalışma kapsamında teşekkür edilecek kişi/kurum bulunmamaktadır.

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Details

Primary Language

English

Subjects

Veterinary Internal Medicine

Journal Section

Research Article

Authors

Yusuf Umut Batı ^*
0000-0001-7528-4376
Türkiye

Mert Sezer
0000-0003-1691-7764
Türkiye

Mustafa Makav
0000-0003-1879-8180
Türkiye

Enes Akyüz
0000-0002-3288-2058
Türkiye

Mushap Kuru
0000-0003-4409-251X
Türkiye

Ali Haydar Kırmızıgül
0000-0002-9283-1391
Türkiye

Early Pub Date

June 11, 2026

Publication Date

June 15, 2026

Submission Date

January 21, 2026

Acceptance Date

April 13, 2026

Published in Issue

Year 2026 Volume: 19 Number: 2

DOI

https://doi.org/10.30607/kvj.1868070

IZ

https://izlik.org/JA29KW95TP

Cite

RIS / Bibtex

APA

Batı, Y. U., Sezer, M., Makav, M., Akyüz, E., Kuru, M., & Kırmızıgül, A. H. (2026). Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis. Kocatepe Veterinary Journal, 19(2), 112-125. https://doi.org/10.30607/kvj.1868070

AMA

1.Batı YU, Sezer M, Makav M, Akyüz E, Kuru M, Kırmızıgül AH. Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis. kvj. 2026;19(2):112-125. doi:10.30607/kvj.1868070

Chicago

Batı, Yusuf Umut, Mert Sezer, Mustafa Makav, Enes Akyüz, Mushap Kuru, and Ali Haydar Kırmızıgül. 2026. “Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle With Traumatic Pericarditis”. Kocatepe Veterinary Journal 19 (2): 112-25. https://doi.org/10.30607/kvj.1868070.

EndNote

Batı YU, Sezer M, Makav M, Akyüz E, Kuru M, Kırmızıgül AH (June 1, 2026) Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis. Kocatepe Veterinary Journal 19 2 112–125.

IEEE

[1]Y. U. Batı, M. Sezer, M. Makav, E. Akyüz, M. Kuru, and A. H. Kırmızıgül, “Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis”, kvj, vol. 19, no. 2, pp. 112–125, June 2026, doi: 10.30607/kvj.1868070.

ISNAD

Batı, Yusuf Umut - Sezer, Mert - Makav, Mustafa - Akyüz, Enes - Kuru, Mushap - Kırmızıgül, Ali Haydar. “Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle With Traumatic Pericarditis”. Kocatepe Veterinary Journal 19/2 (June 1, 2026): 112-125. https://doi.org/10.30607/kvj.1868070.

JAMA

1.Batı YU, Sezer M, Makav M, Akyüz E, Kuru M, Kırmızıgül AH. Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis. kvj. 2026;19:112–125.

MLA

Batı, Yusuf Umut, et al. “Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle With Traumatic Pericarditis”. Kocatepe Veterinary Journal, vol. 19, no. 2, June 2026, pp. 112-25, doi:10.30607/kvj.1868070.

Vancouver

1.Yusuf Umut Batı, Mert Sezer, Mustafa Makav, Enes Akyüz, Mushap Kuru, Ali Haydar Kırmızıgül. Changes in Malondialdehyde, Reduced Glutathione, and Thiol–Disulfide Homeostasis in Cattle with Traumatic Pericarditis. kvj. 2026 Jun. 1;19(2):112-25. doi:10.30607/kvj.1868070