Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species

Hakan Işık; Ceylan Özsoy; Uğur Tutar; Emine Dinçer

doi:10.30708/mantar.1469051

EN TR

Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species

Abstract

In this study, ethanol extracts of some wild mushroom species, in the phylum Basidiomycota, (Armillaria mellea (Vahl) P. Kumm., Infundibulicybe geotropa (Bull.) Harmaja, Leucopaxillus gentianeus (Quél.) Kotl. and Trametes versicolor (L.) Lloyd) were tested for their antioxidant, antimicrobial, antibiofilm, and cytotoxic activities. Mushroom samples showed low antimicrobial activity on Enterococcus faecalis and Staphylococcus aureus, while biofilm inhibitory activity on test microorganisms ranged from 24.6% to 80.5%. At the end of the antioxidant activity studies, A. mellea was the mushroom sample having the highest DPPH radical scavenging capacity (0.105±0.001 mg/mL) whereas T. versicolor was the one having the highest iron (III) ion reducing power (40.709±0.003 µg TE/100g). The highest polyphenol content was observed in T. versicolor (29.916±0.002 mg GAE/100g) samples, and the lowest in A. mellea (9.5±0.006 mg GAE/100g) samples. The cytotoxic effects of the samples were tested on MCF-7, MDA-MB-231 (breast cancer) and L929 (mouse fibroblast) cell lines using the MTT method. As a result, it was observed that A. mellea and T. versicolor samples were more effective on the MCF-7 cell line, and A. mellea on MDA-MB-231 cell line.

Keywords

Project Number

ECZ-076

Bazı Doğal Mantar Türlerinin Antibiyofilm, Antimikrobiyal, Sitotoksik ve Antioksidan Etkilerinin Değerlendirilmesi

Abstract

Bu çalışmada Basidiomycota şubesinde yer alan bazı doğal mantar türlerinin (Armillaria mellea (Vahl) P. Kumm., Infundibulicybe geotropa (Bull.) Harmaja, Leucopaxillus gentianeus (Quél.) Kotl. ve Trametes versicolor (L.) Lloyd) etanol ekstraktları, antioksidan, antimikrobiyal, antibiyofilm ve sitotoksik aktiviteleri açısından test edildi. Mantar örnekleri Enterococcus faecalis ve Staphylococcus aureus üzerinde düşük antimikrobiyal aktivite gösterirken, test mikroorganizmaları üzerinde %24.6 ile %80.5 arasında değişen biyofilm önleyici aktivite gösterdi. Antioksidan aktivite çalışmaları sonucunda, A. mellea en yüksek DPPH radikal temizleme kapasitesine (0.105±0.001 mg/mL), T. versicolor ise en yüksek demir (III) iyonu indirgeme gücüne (40.709± 0.003 µg TE/100g) sahip mantar türü olmuştur . En yüksek polifenol içeriği T. versicolor (29,916±0,002 mg GAE/100g) örneklerinde, en düşük ise A. mellea (9,5±0,006 mg GAE/100g) örneklerinde gözlendi. Örneklerin sitotoksik etkileri MCF-7, MDA MB-231 (meme kanseri) ve L929 (fare fibroblast) hücre hatlarında MTT yöntemi kullanılarak test edildi. Sonuç olarak A. mellea ve T. versicolor örneklerinin MCF-7 hücre hattında, A. mellea'nın MDA-MB-231 hücre hattında daha etkili olduğu görüldü.

Keywords

Project Number

ECZ-076

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Details

Primary Language

English

Subjects

Mycology

Journal Section

Research Article

Authors

Hakan Işık ^*
0000-0001-8241-0078
Türkiye

Ceylan Özsoy
0000-0001-6397-1291
Türkiye

Uğur Tutar
0000-0002-8058-0994
Türkiye

Emine Dinçer
0000-0002-6361-4419
Türkiye

Publication Date

October 14, 2024

Submission Date

April 21, 2024

Acceptance Date

May 23, 2024

Published in Issue

Year 2024 Volume: 15 Number: 2

DOI

https://doi.org/10.30708/mantar.1469051

IZ

https://izlik.org/JA93ZP89JG

Cite

RIS / Bibtex

APA

Işık, H., Özsoy, C., Tutar, U., & Dinçer, E. (2024). Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species. Mantar Dergisi, 15(2), 60-69. https://doi.org/10.30708/mantar.1469051

AMA

1.Işık H, Özsoy C, Tutar U, Dinçer E. Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species. Mantar Dergisi. 2024;15(2):60-69. doi:10.30708/mantar.1469051

Chicago

Işık, Hakan, Ceylan Özsoy, Uğur Tutar, and Emine Dinçer. 2024. “Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species”. Mantar Dergisi 15 (2): 60-69. https://doi.org/10.30708/mantar.1469051.

EndNote

Işık H, Özsoy C, Tutar U, Dinçer E (October 14, 2024) Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species. Mantar Dergisi 15 2 60–69.

IEEE

[1]H. Işık, C. Özsoy, U. Tutar, and E. Dinçer, “Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species”, Mantar Dergisi, vol. 15, no. 2, pp. 60–69, Oct. 2024, doi: 10.30708/mantar.1469051.

ISNAD

Işık, Hakan - Özsoy, Ceylan - Tutar, Uğur - Dinçer, Emine. “Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species”. Mantar Dergisi 15/2 (October 14, 2024): 60-69. https://doi.org/10.30708/mantar.1469051.

JAMA

1.Işık H, Özsoy C, Tutar U, Dinçer E. Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species. Mantar Dergisi. 2024;15:60–69.

MLA

Işık, Hakan, et al. “Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species”. Mantar Dergisi, vol. 15, no. 2, Oct. 2024, pp. 60-69, doi:10.30708/mantar.1469051.

Vancouver

1.Hakan Işık, Ceylan Özsoy, Uğur Tutar, Emine Dinçer. Evaluation of Antibiofilm, Antimicrobial, Cytotoxic and Antioxidant Effects of Some Wild Mushroom Species. Mantar Dergisi. 2024 Oct. 14;15(2):60-9. doi:10.30708/mantar.1469051