@article{article_1056629, title={Investigation of antioxidant and antimicrobial activities of walnut (Juglans regia L.) kernel septum}, journal={The European Research Journal}, volume={9}, pages={87–96}, year={2023}, DOI={10.18621/eurj.1056629}, author={Özşahin Delibaş, Elif Azize and Kıray, Esin}, keywords={Juglans regia L, walnut kernel septum, antioxidant, total phenolic content, antimicrobial activity}, abstract={<p> <b>Objectives: </b>Walnut ( <i>Juglans regia </i> L.) kernel septum (or septa) (WKS), a traditional nutraceutical material in China, has not been explored in detail. In this study, antimicrobial activity, total phenolic content (TPC) and antioxidant-oxidant status of WKS was investigated in case it may be clinically important in the management of various complications. </p> <p> <b>
Methods: </b> The WKS was extracted with ethanol in a Soxhlet device. TPC of WKS was analysed by using Folin-Ciocalteu’s method. Antioxidant activity was obtained by using Rel Assay Diagnostics kits. The antimicrobial activity of WKS was evaluated against two Gram-positive ( <i>Staphylococcus aureus, Bacillus subtilis </i>), one Gram-negative bacteria ( <i>Escherichia coli </i>) and one fungus ( <i>Candida albicans </i>) strains using the agar diffusion method. </p> <p> <b>Results: </b>The TPC of WKS was found to be 119.42 ± 2.39 mg GAE/gDW. It was determined that total antioxidant status (TAS), total oxidant status (TOS) and oxidative stress index (OSI) values were 7.542 ± 0.389 mmol/L, 3.718 ± 0.287 µmol/L and 0.049 ± 0.001, respectively. WKS selectively inhibited the growth of Gram-positive bacteria and fungus, while <i>S. aureus </i> was the most susceptible one with 16 mm of inhibition zone. Gram-negative bacteria was resistant to the extract. </p> <p> <b>Conclusions: </b>As far as we know, this paper is the first work that demonstrates the antioxidant-oxidant status of WKS by using the method described above, and moreover there are no scientific reports which have examined WKS in such a multidisciplinary experimental design. This study strongly supports the reported traditional use of WKS. Results indicated that WKS can be used as a pharmacological natural agent due to its high antioxidant and antimicrobial activities. </p>}, number={1}, publisher={Prusa Medical Publishing}