@article{article_1058051, title={Experimental Design Optimized Chromatographic Determination of Pseudoephedrine and Cetirizine in Bulk and Tablets}, journal={Hacettepe University Journal of the Faculty of Pharmacy}, volume={42}, pages={165–174}, year={2022}, DOI={10.52794/hujpharm.1058051}, author={Reid, Imad}, keywords={Pseudoephedrine, Cetirizine, HPLC, Experimental design}, abstract={Chromatographic separation of cetirizine and pseudoephedrine was established using cyanopropyl column and mobile phase composed of aqueous phase (20 mM ammonium acetate buffer, pH 5.5) and acetonitrile (44:56v/v) pumped at 1 mL/minute, analytes were detected at 240 nm. The linearity of the method was between 25- 135 μg/mL and 1.5 –7.5 μg/mL for pseudoephedrine and cetirizine, respectively. Pseudoephedrine and cetirizine detection limits were 5.34 and 0.13 μg/mL, respectively, whereas their quantitation limits were 16.17 μg/mL and 0.40 μg/mL for cetirizine. The method’s recoveries were (99.81% ± 0.60 for pseu- doephedrine and 100.08 ± 0.52 for cetirizine), indicating consistent good and consistent recoveries. Also low relative standard deviations (< 2%) for repeatabil- ity and intermediate precision were obtained supporting method’s high precision. Quantification of pseudoephedrine and cetirizine in bulk and combined tablet formulation was successfully carried out using the validated method.}, number={3}, publisher={Hacettepe University}, organization={None}