TY  - JOUR
T1  - Diagnosis of bacterial fish diseases and classification of serotypes with slide agglutination method
TT  - Lam aglütinasyon metodu ile bakteriyel balık hastalıklarının teşhisi ve serotiplerin sınıflandırılması
AU  - Kumaş, Kaan
AU  - Tanrıkul, Prof.dr. T. Tansel
PY  - 2022
DA  - June
DO  - 10.12714/egejfas.39.2.02
JF  - Ege Journal of Fisheries and Aquatic Sciences
JO  - EgeJFAS
PB  - Ege University
WT  - DergiPark
SN  - 2148-3140
SP  - 97
EP  - 103
VL  - 39
IS  - 2
LA  - en
AB  - Bacterial fish pathogens cause significant losses in rainbow trout farms. In fish farms, bacterial pathogens cause threatening diseases which have made necessary to develop rapid methods for disease diagnosis. Serological techniques which are applied with a small amount of antiserum and sample, are preferred for the rapid diagnosis of fish diseases. In this study, formalin-killed antigens prepared from reference strains of Lactococcus garvieae, Yersinia ruckeri, and Vibrio (Listonella) anguillarum were injected intravenously in consecutive doses to New Zealand rabbits. One week after the last injection, the sera separated to use in the slide agglutination tests. A total of 42 strains were studied, including Y. ruckeri (18 isolates), V. anguillarum (14 isolates), and L. garvieae (7 isolates) and 3 reference (ATCC 43305, ATCC 29473, ATCC 49156) strains. Serotype O1 determined predominant serotype (86%) in V. anguillarum and Y. ruckeri (84%) strains examined by slide agglutination method. L. garvieae strains did not react against Japanese antisera but positively reacted against Turkish L. garvieae antisera.
KW  - Bacterial fish pathogens
KW  - diagnosis
KW  - serological characterization
N2  - Bakteriyel balık patojenleri gökkuşağı alabalığı yetiştiriciliği yapan balık çiftliklerinde ciddi kayıplara sebep olmaktadır. Su ürünleri yetiştiriciliğinde hastalık etkeni bakteriyel patojenler, oluşan hastalıkların teşhisi için hızlı teşhis yöntemlerinin gelişmesine yol açmıştır. Hastalıklar yetiştiricilik tesislerinde görüldükten sonra balık patojenlerinin hızlı teşhis edilmesi, hastalıkların tedavi edilerek oluşacak ekonomik kayıpların önüne geçilmesi için önemlidir. Balık hastalıklarının hızlı teşhisinde az miktarda antiserum ve örnek ile uygulanabilen serolojik teknikler tercih edilmektedir. Bu çalışmada Lactococcus garvieae, Vibrio (Listonella) anguillarum ve Yersinia ruckeri’nin referans suşlarından antijenler kullanılmak üzere formalin ile inaktive edilerek, Yeni Zelanda tavşanlarına ardışık dozlarda intravenöz enjeksiyonlar gerçekleştirilmiştir. Son enjeksiyon yapıldıktan bir hafta sonra antiserum elde edilmiştir. 18 Y. ruckeri, 14 V. anguillarum ve 7 L. garvieae ve referans (ATCC 43305, ATCC 29473, ATCC 49156) suşlar dahil olmak üzere toplamda 42 suş ile çalışma yapılmıştır. Lam aglütinasyon metodu ile incelenen V. anguillarum suşlarında baskın olarak (%86) serotip O1, Y. ruckeri suşlarında (%84) serotip O1 tespit edilmiştir. L. garvieae suşlarının Japon KG- antiserumu ile aglütinasyon vermediği, ancak Türk KG- antiserumu ile pozitif reaksiyon oluşturduğu tespit edilmiştir.
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UR  - https://doi.org/10.12714/egejfas.39.2.02
L1  - https://dergipark.org.tr/en/download/article-file/2205759
ER  -