@article{article_1075016, title={The investigation of the presence of Listeria species in poultry farms and antimicrobial resistance profiles of Listeria monocytogenes strains}, journal={Journal of Istanbul Veterinary Sciences}, volume={6}, pages={26–34}, year={2022}, DOI={10.30704/http-www-jivs-net.1075016}, author={Çokal, Yavuz and Günaydın, Elçin and Goncagül, Gülşen}, keywords={Listeria spp., broiler, layer flock, prevalence, antimicrobial resistance}, abstract={<div style="text-align:justify;"> <i>Listeria </i> species are widespread in nature and found in various environments. In this study, the presence of <i>Listeria </i> species in poultry farms was investigated. For this purpose, a total of 332 samples including feces, feed, drinking water and nipple swab from 5 broiler flocks reared on the floor and 352 samples including feces, feed, drinking water and eggshell swab from 5 layer flocks reared in cages were obtained. A modified version of the USDA-FSIS MLG 8.13 method was used for the isolation of <i>Listeria </i> species from samples. As a result of the study, Listeria spp. was isolated from all broiler and layer flocks. Isolation of <i>Listeria </i> spp. was carried out from 18 of 190 feces samples (9.4%), 5 of 15 drinking water samples (33.3%), 3 of 102 nipple swab samples (2.9%) in broiler flocks, and 22 of 167 feces samples (13.1%), 2 of 25 feed samples (8%), 3 of 15 drinking water samples (20%), 3 of 145 eggshell swab samples (2%) in layer flocks. Isolates were identified by cultural and biochemical characters, and a total of 56 <i>Listeria </i> isolates were identified as 15 <i>L. monocytogenes </i>, 3 <i>L. ivanovii </i>, 19 <i> L. innocua </i>, 13 <i> L. seeligeri </i>, 2 <i>L. welshimeri </i>, and 4 <i>L. grayi </i>. The antibiotic resistance profiles of <i>L. monocytogenes </i> isolates to twelve antibiotics were detected by the disc diffusion method. <i>L. monocytogenes </i> isolates were found to exhibit the highest resistance to ciprofloxacin (33.3%) among twelve antibiotics, and three isolates (20%) were also multidrug resistant. Consequently, it was determined that <i> Listeria </i> species in poultry farms are common. </div>}, number={1}, publisher={The Chamber of Veterinary Surgeons, Istanbul}