@article{article_1081989, title={DETERMINATION OF CHROMATOGRAPHIC PROTONATION CONSTANTS OF SOME IMIDAZOLE ANTIMYCOTIC DRUGS IN ACETONITRILE-WATER BINARY MIXTURES}, journal={Journal of Faculty of Pharmacy of Ankara University}, volume={46}, pages={405–417}, year={2022}, DOI={10.33483/jfpau.1081989}, author={Seçilmiş, Hayrettin and Çubuk Demiralay, Ebru and Poturcu, Kader}, keywords={Combined effect, Imidazole, Nonlinear regression, RPLC}, abstract={<div style="text-align:justify;"> <i>Objective: In this study, the thermodynamic protonation constant ((_s^s)〖pK〗_a ) of antifungal azole derivative miconazole and tioconazole were investigated by the reversed-phase liquid chromatography (RPLC) method. Although classical stationary phases are the most commonly used column-packed materials in the analyses of these compounds by the RPLC method, the determination of the pKa value of miconazole and tioconazole with a cyano-based column has not been reported so far. </i> </div> <i> </i> <div style="text-align:justify;"> <i> <i>Material and Method: Chromatographic behavior of the selected compounds was determined with Ultra Cyano column (Restek®, 150×3.0 mm I.D, 3µm) which is compatible with hydrophobic compounds. The dependence of the retention times on the acetonitrile content of the mobile phase and the effect of the mobile phase pH on the chromatographic retention was determined for each compound by changing the pH in the range of 3.0-7.50. </i> </i> </div> <i> </i> <div style="text-align:justify;"> <i> <i>Result and Discussion: The (_s^s)〖pK〗_a values were determined by a nonlinear regression program. The thermodynamic aqueous pKa ((_w^w)〖pK〗_a ) value of the studied compounds was calculated from the (_s^s)〖pK〗_a value by using the macroscopic parameters (dielectric constant and mole fraction) that play an important role in the solvent properties. The calculated (_w^w)〖pK〗_a values were compared with literature values. </i> </i> </div> <i> </i>}, number={2}, publisher={Ankara University}