@article{article_1091707, title={Effect of geraniol and vitamin C on diethylnitrosamine induced experimental hepatocellular carcinogenesis}, journal={Cukurova Medical Journal}, volume={47}, pages={738–746}, year={2022}, DOI={10.17826/cumj.1091707}, author={Taşkın Şenol, Gamze and Kaplan, Halil Mahir and Boyan, Neslihan and Oğuz, Özkan and Şingirik, Ergin}, keywords={Cancer, cell culture, hepatocytes, liver, mediators, pathway}, abstract={Purpose: This study aimed to investigate the protective effect of geraniol and vitamin C on the experimental hepatocellular carcinogenesis (HCC) model by inducing FL83B hepatocyte cell lines with diethylnitrosamine (DENA). Materials and Methods: The cells prepared in the medium were incubated with DENA (5 μM), geraniol (5 μM), and vitamin C (50 μM) for 48 hours in an incubator at 37 °C and 5% CO2. Groups were designed as follows: Group 1 (Control), group 2 (DENA Control), group 3 (DENA+Geraniol), group 4 (DENA+Vitamin C), and group 5 (DENA+Geraniol+Vitamin C) on standard cell culture plates. Six plates from each experimental group were studied. After the homogenization was centrifuged, analyses of pathway mediators NF-ĸB, AIF, caspase-3, BCL-2, bax, gadd153, GRP78, and COX were performed by the Elisa method. Results: The expression of Bax, caspase-3, COX-2, NFkB, GADD153, AIF, and GRP78 increased in cancer cells when compared to group 1 and decreased in other groups where antiproliferative agents were applied. Bcl-2 expression is decreased when compared to group 1, and expression is increased in other groups where antiproliferative agents are applied. Conclusion: There was a significant hepatoprotective effect in the groups administered geraniol+vitamin C on pathway mediators in a DENA-induced HCC model.}, number={2}, publisher={Cukurova University}, organization={Çukurova Üniversitesi Bilimsel Araştırmalar Projeleri Bİrimi}