TY  - JOUR
T1  - Ratlarda Oluşturulan Kritik Boyutlu Kalvaryal Defektlerde Bakteriyel Selüloz Temelli Hidroksiapatit (BS-HAp) Nanokompozitinin Kemik Oluşumu Üzerine Etkisi
TT  - The Effect of Bacterial Cellulose Based Hydroxyapatite (BC-HAp) Nanocomposite on Bone Formation in Critical Sized Calvarial Defects in Rats
AU  - Lektemür Alpan, Aysan
AU  - Dogan, Nazime
AU  - Denizli, Tuğba Hilal
AU  - Özmen, Özlem
PY  - 2022
DA  - December
DO  - 10.7212/karaelmasfen.1103092
JF  - Karaelmas Fen ve Mühendislik Dergisi
PB  - Zonguldak Bulent Ecevit University
WT  - DergiPark
SN  - 2146-7277
SP  - 242
EP  - 250
VL  - 12
IS  - 2
LA  - tr
AB  - Bu çalışmada, sıçanlarda kritik boyuttaki kalvaryal defektlerde bakteriyel selüloz-hidroksiapatit (BS-HAp) kompozitinin kemik rejenerasyonundaki potansiyelinin değerlendirilmesi amaçlanmıştır. Komagataibacter xylinus S4 izolatından elde edilen BS-HAp taramalı elektron mikroskobunda (SEM) incelenerek içeriğindeki Ca ve P iyonlarının yoğunluğu tespit edilmiştir. Ratlarda oluşturulan kalvaryal defektlere elde edilen BS-HAp dondurulup kurutularak uygulanmıştır. 4. ve 8. haftanın sonunda alınan örnekler histopatolojik olarak incelenmiştir. SEM sonuçlarına göre BS lifleri, ince demetler halinde olup fibril çapı 42,11olarak tespit edilmiştir. HAp, BS pelikülüne dahil olarak içeriğinde yüksek oranda Ca ve P elementleri saptanmıştır. Yapılan in-vivo deneylerin sonunda 4. Haftada herhangi bir kemik oluşumuna rastlanmamıştır. BS-HAp grubunda düzgün bağ doku oluşumu gözlenmiştir. 8. haftanın sonunda BS-HAp grubunda biyomateryal komşuluğunda yeni kemik alanları izlenmiştir. Komagataibacter xylinus S4 izolatından elde edilen BS-HAp biyomateryal olarak kullanıldığında 8. haftada yeni kemik oluşumunu indüklemiştir
KW  - Bakteriyel selüloz
KW  - biyomateryal
KW  - hidroksiapatit
KW  - kemik rejenerasyonu
KW  - Bacterial cellulose
KW  - Biomaterial
KW  - Hydroxyapatite
KW  - Bone regeneration
N2  - In this study, it was aimed to evaluate the potential bone regeneration effect of bacterial cellulose-hydroxyapatite (BC-HAp) composite in calvarial defects of critical size in rats. BC-HAp obtained from Komagataibacter xylinus S4 isolate was examined under a scanning electron microscope (SEM), and the concentration of Ca and P ions in its content was determined. BC-HAp obtained from calvarial defects in rats was freeze-dried and applied. Samples taken at the end of the 4th and 8th weeks were examined histopathologically. According to SEM results, BC fibers were in thin bundles and the fibril diameter was determined as 42.11. As HAp is included in the BC pellicle, high levels of Ca and P elements were detected in its content. At the end of the in-vivo experiments, no bone formation was found in the 4th week. Uniform connective tissue formation was observed in the BS-HAp group. At the end of the 8th week, new bone areas were observed in the BS-HAp group adjacent to the biomaterial. When BS-HAp obtained from Komagataibacter xylinus S4 isolate was used as a biomaterial, it induced new bone formation at 8 weeks
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UR  - https://doi.org/10.7212/karaelmasfen.1103092
L1  - https://dergipark.org.tr/en/download/article-file/2372801
ER  -