@article{article_1124000, title={Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions}, journal={Experimed}, volume={12}, pages={85–93}, year={2022}, DOI={10.26650/experimed.1124000}, author={Kalaycı Yüksek, Fatma and Gümüş, Defne and Gündoğan, Gül İpek and Uyanık Öcal, Aysun and Elagül, Nur and Anğ Küçüker, Mine}, keywords={Norepinephrine, MRSA, growth, adhesion-invasion, oxidative stress response, HOB cell viability, apoptosis-necrosis}, abstract={<p>Objective: The role of norepinephrine (NE) on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300 was examined in human osteoblast (HOB) cells. The effects of NE and/or MRSA on the viability and cell death pathways of HOB cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress (H2O2) were analyzed in the presence/absence of NE. </p> <p>Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesion-invasion. The alteration of HOB cell viability was determined by methyl thiazolyl diphenyl-tetrazolium bromide (MTT) assay. The death pathways of HOB cells were examined microscopically using acridine orange-ethidium bromide dual staining and dichlorofluorescein-diacetate (DCF-DA) dye. The bacterial response to H2O2 was investigated by agar dilution. </p> <p>Results: The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE (p<0.0001) while high-level NE induced invasion (p=0.013). HOB cell viability was reduced by MRSA and/or NE (p<0.001). MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells (p<0.05). NE did not alter the bacterial response to oxidative stress. </p> <p>Conclusion: Norepinephrine has different effects on the biological properties of both MRSA and HOB cells. </p>}, number={2}, publisher={Istanbul University}