TY  - JOUR
T1  - Cross-interactions between Norepinephrine, Methicillin-Resistant Staphylococcus aureus and Human Osteoblast Cells in Culture Conditions
AU  - Kalaycı Yüksek, Fatma
AU  - Gümüş, Defne
AU  - Gündoğan, Gül İpek
AU  - Uyanık Öcal, Aysun
AU  - Elagül, Nur
AU  - Anğ Küçüker, Mine
PY  - 2022
DA  - August
DO  - 10.26650/experimed.1124000
JF  - Experimed
PB  - Istanbul University
WT  - DergiPark
SN  - 2667-5846
SP  - 85
EP  - 93
VL  - 12
IS  - 2
LA  - en
AB  - Objective: The role of norepinephrine (NE) on growth, adhesion and invasion of methicillin-resistant Staphylococcus aureus (MRSA) ATTC 43300  was  examined  in  human  osteoblast  (HOB)  cells.  The  effects  of  NE  and/or  MRSA  on  the  viability  and  cell  death  pathways  of  HOB  cells were also investigated. Furthermore, the alterations of bacterial response to oxidative stress (H2O2) were analyzed in the presence/absence of NE. Materials and Methods: Bacterial growth was detected spectrophotometrically. The colony counting method was examined for adhesion-invasion.  The  alteration  of  HOB  cell  viability  was  determined  by  methyl  thiazolyl  diphenyl-tetrazolium  bromide  (MTT)  assay.  The  death  pathways  of  HOB  cells  were  examined  microscopically  using  acridine  orange-ethidium  bromide  dual  staining  and  dichlorofluorescein-diacetate (DCF-DA) dye. The bacterial response to H2O2 was investigated by agar dilution.Results: The growth of bacterium was not affected in the presence of NE. Bacterial adhesion was decreased by NE (p<0.0001) while high-level NE induced invasion (p=0.013). HOB cell viability was reduced by MRSA and/or NE (p<0.001). MRSA and co-existence of MRSA and NE caused necrosis more than apoptosis in HOB cells (p<0.05). NE did not alter the bacterial response to oxidative stress. Conclusion: Norepinephrine has different effects on the biological properties of both MRSA and HOB cells.
KW  - Norepinephrine
KW  - MRSA
KW  - growth
KW  - adhesion-invasion
KW  - oxidative stress response
KW  - HOB cell viability
KW  - apoptosis-necrosis
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UR  - https://doi.org/10.26650/experimed.1124000
L1  - https://dergipark.org.tr/en/download/article-file/2458361
ER  -