TY  - JOUR
T1  - TRANSCRIPTOMIC DIFFERENCES IN MONOZYGOTIC TWINS WITH OVARIAN CANCER
TT  - TRANSCRIPTOMIC DIFFERENCES IN MONOZYGOTIC TWINS WITH OVARIAN CANCER
AU  - Kuru Türkcan, Gözde
AU  - Akdeniz Ödemiş, Demet
AU  - Kurt, Büşra
AU  - Tuncer, Buğra
AU  - Kılıç Erciyas, Seda
AU  - Şükrüoğlu Erdoğan, Özge
AU  - Yazıcı, Hülya
PY  - 2022
DA  - August
DO  - 10.26650/JARHS2021-1137404
JF  - Journal of Advanced Research in Health Sciences
JO  - SABİAD
PB  - Istanbul University
WT  - DergiPark
SN  - 2651-4060
SP  - 36
EP  - 36
VL  - 5
IS  - S-1
LA  - en
AB  - Objectives: The most significant barrier to treating epithelial ovarian cancer(EOC), is the late diagnosis and the emergence of chemotherapy resistance inthe early stages.The ineffectiveness of standard treatment protocols in advanced-stage cases highlights the value of individualized EOC treatment.Evenmonozygotic(MZ) twins, with almost the same genotype in nature, can develop different phenotypic characteristics throughout life.Materials-Methods: In this context, the peripheral blood and ovarian tissues of monozygotic twins who are discordant in terms of ovarian cancer and theother healthy sibling who is a BRCA1 mutation carrier were compared in the study to analyze all RNA molecules transcribed at the genome level usingnext-generation sequencing technology.Through analyzing the molecules transcribed in the whole genome,the genetic variations underlying thedevelopment of ovarian cancer in a high-risk family bearing the BRCA1 mutation but only one of whom was found to have ovarian cancer were examined.Results: As a result of the transcriptomic analysis of the total RNA of 6 samples obtained from the ovarian tissues and peripheral blood samples of MZtwins with ovarian cancer and healthy sibling, the protein-encoding protein that shows expression changes in tissue and blood compared to healthy peoplegenes,lncRNAs,precursor-miRNAs and fusion-transcripts have been identified.Filtering procedures and expression change coefficients(log2Fc) werecalculated based on the expression data of identified ovarian cancer tumor markers CA125 and CA15-3.Conclusions: According to our study the expression levels of 66 protein-coding genes,23 novel lncRNAs,9 miRNAs and,2 fusion transcripts were found tovary in three groups and,these molecules are thought to be candidate molecules associated with epithelial ovarian cancer.
KW  - monozygotic twins
KW  - Epithelial Ovarian Cancer
KW  - RNA Sequence Analysis
KW  - Gene Expression Profiling
N2  - Objectives: The most significant barrier to treating epithelial ovarian cancer(EOC), is the late diagnosis and the emergence of chemotherapy resistance inthe early stages.The ineffectiveness of standard treatment protocols in advanced-stage cases highlights the value of individualized EOC treatment.Evenmonozygotic(MZ) twins, with almost the same genotype in nature, can develop different phenotypic characteristics throughout life.Materials-Methods: In this context, the peripheral blood and ovarian tissues of monozygotic twins who are discordant in terms of ovarian cancer and theother healthy sibling who is a BRCA1 mutation carrier were compared in the study to analyze all RNA molecules transcribed at the genome level usingnext-generation sequencing technology.Through analyzing the molecules transcribed in the whole genome,the genetic variations underlying thedevelopment of ovarian cancer in a high-risk family bearing the BRCA1 mutation but only one of whom was found to have ovarian cancer were examined.Results: As a result of the transcriptomic analysis of the total RNA of 6 samples obtained from the ovarian tissues and peripheral blood samples of MZtwins with ovarian cancer and healthy sibling, the protein-encoding protein that shows expression changes in tissue and blood compared to healthy peoplegenes,lncRNAs,precursor-miRNAs and fusion-transcripts have been identified.Filtering procedures and expression change coefficients(log2Fc) werecalculated based on the expression data of identified ovarian cancer tumor markers CA125 and CA15-3.Conclusions: According to our study the expression levels of 66 protein-coding genes,23 novel lncRNAs,9 miRNAs and,2 fusion transcripts were found tovary in three groups and,these molecules are thought to be candidate molecules associated with epithelial ovarian cancer.
CR  - Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
UR  - https://doi.org/10.26650/JARHS2021-1137404
L1  - https://dergipark.org.tr/en/download/article-file/2513515
ER  -