TY  - JOUR
T1  - METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
TT  - METHYLATION AND EXPRESSION VALIDATION RESULTS OF GPRC5C GENE OBTAINED FROM EPIGENOMICS AND TRANSCRIPTOMICS DATA IN ORAL CAVITY TUMORS
AU  - Esentürk, Gizem
AU  - Doruk, Can
AU  - Ulusan, Murat
AU  - Olgaç, Nejat Vakur
AU  - Demokan, Semra
PY  - 2022
DA  - August
DO  - 10.26650/JARHS2021-1138086
JF  - Journal of Advanced Research in Health Sciences
JO  - SABİAD
PB  - Istanbul University
WT  - DergiPark
SN  - 2651-4060
SP  - 46
EP  - 46
VL  - 5
IS  - S-1
LA  - en
AB  - Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression andmethylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expressionstatus of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker wasevaluated for early diagnosis of OSCC by non-invasive methods.Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation andexpression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated bystatistical analysis.Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared tocorresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%.We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degreeand expression in OSCC.Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Othermechanisms need to be explored to understand the differences in expression levels.
KW  - gene expression
KW  - DNA methylation
KW  - OSCC
KW  - GPRC5C
N2  - Objective: Demokan et.al idetified G/Protein/Coupled/Receptor/Class-C/Group-5/Member-C (GPRC5C) as a candidate gene in OSCC via expression andmethylation array results (logFC:0.59663; DeltaBeta: -0.130984561) from their previous unpublished data. In our study, the methylation and expressionstatus of GPRC5C gene was investigated in a larger group of OSCC patients and healthy cohort for further validation. Its potential candidate biomarker wasevaluated for early diagnosis of OSCC by non-invasive methods.Materials and Methods: DNA and RNA were isolated from tissues/body fluid samples of 30 patients OSCC and 15 healthy individuals. The methylation andexpression levels were analyzed by using QMSP and QRT-PCR methods, respectively. The data were compared with clinical parameters and evaluated bystatistical analysis.Results: Decreased expression levels were observed in 43% and increased expression levels were observed in 33% in the tumor tissues compared tocorresponding-normal tissues of OSCC patients. Methylation-based expression loss was observed in 4 patients having the ratio between 3.4 and 12.2%.We also observed a decrease in expression in OSCC patients with observed methylation. There was statistical significance between differentiation degreeand expression in OSCC.Conclusion: It was concluded that GPRC5C may play a role as a potential methylation biomarker candidate of specific subgroups of the oral cavity. Othermechanisms need to be explored to understand the differences in expression levels.
CR  - Grozescu T, Popa F. Prostate cancer between prognosis and adequate/proper therapy. J Med Life. 2017;10(1):5-12
UR  - https://doi.org/10.26650/JARHS2021-1138086
L1  - https://dergipark.org.tr/en/download/article-file/2516157
ER  -