TY - JOUR T1 - Determination of reticular fibers in tissues fixed with sugarcane molasses AU - Kuloğlu, Hatice Yaren PY - 2022 DA - December Y2 - 2022 DO - 10.31797/vetbio.1164030 JF - Journal of Advances in VetBio Science and Techniques JO - J Ad VetBio Sci Tech PB - İlker ÇAMKERTEN WT - DergiPark SN - 2548-1150 SP - 361 EP - 365 VL - 7 IS - 3 LA - en AB - Reticular fiber consists of one or more types of very thin and delicately woven type III collagen strands, which form a highly ordered cellular network and provide a supportive network. Since most of these collagen types are combined with carbohydrates, they react with silver stains and periodic acid-Schiff reagent, but are not shown by ordinary histological stains like those using hematoxylin. In this study, it was aimed to determine the reticular fibers in tissues fixed with sugarcane molasses and formol using silver staining technique (Gordon Sweet –GS-). The reticular fibers in the tissues fixed with sugarcane molasses were compared with those in the tissues fixed with formol. According to the findings, the staining quality of the liver and spleen tissues fixed with sugarcane molasses showed similar characteristics with the tissues fixed with formol. Very weak staining was observed in kidney and testicle tissues. The fact that the tissues fixed in the same fixation solution show different results in the Gordon Sweet (GS) staining method shows us that this issue needs to be supported by more detailed studies. KW - Reticular fibers KW - fixing KW - sugarcane molasses KW - formol CR - Alvarenga, H. G. and Marti, L. (2014). Multifunctional Roles of Reticular Fibroblastic Cells: More Than Meets the Eye?, Journal of Immunology Research, Vol. 10, pp 11-15 CR - Gelse, K. (2003). Collagens-structure, function, and biosynthesis, Advanced Drug Delivery Reviews, Vol. 55, No.12, pp 1531-1546 CR - Kumar, V., Abbas, K. A., Aster, C. J. Robbins and Cotran, Pathologic Basis of Disease, 9th edition, Elsevier Saunders Mescher Anthony L. Junqueira’s Basic Histology: Text and Atlas, 13th edition, McGraw-Hill Education CR - Pepin, M. et al. (2000). Clinical and Genetic Features of Ehlers–Danlos Syndrome Type IV, the Vascular Type, New England Journal of Medicine, Vol. 342, No. 10, pp 673-680 CR - Ross, M. H. and Pawlina, W. Histology: A text and atlas: With Correlated Cell and Molecular Biology, 6th edition, Lippincott Williams & Wilkins CR - Williams, P. A. (1947). Creative Reading, The English Journal, Vol. 36, No. 9 (Nov, 1947), pp 454-459 CR - Young B., Woodford P., O’Dowd G. Wheater’s Functional Histology: A Text and Colour Atlas, 6th edition, Churchill Livingstone Elsevier UR - https://doi.org/10.31797/vetbio.1164030 L1 - https://dergipark.org.tr/en/download/article-file/2604432 ER -