TY - JOUR T1 - The Effect of Whey Proteins on the Brain and Small Intestine Nitric Oxide Levels: Protein Profiles in Methotrexate-Induced Oxidative Stress AU - Tunalı-akbay, Tuğba AU - Yılmaz, Sümeyye AU - Tufan, Elif AU - Sivas, Güzin Göksun AU - Gürel Gökmen, Begüm AU - Dursun, Ercan AU - Özbeyli, Dilek AU - Şener, Göksel PY - 2022 DA - December DO - 10.26650/experimed.1189898 JF - Experimed PB - Istanbul University WT - DergiPark SN - 2667-5846 SP - 113 EP - 118 VL - 12 IS - 3 LA - en AB - Objectives: The aim of this study was to determine the effects of whey proteins on methotrexate (MTX)-induced brain and small intestine damage.Materials and Methods: 30 Sprague Dawley rats (200-300 g) were divided into four groups: Control, control + whey, MTX, and MTX+whey. MTX was administered at 20 mg/kg (single dose) intraperitoneally to the MTX group rats, and 2 mg/kg of whey protein were administered by oral gavage for 10 days to the whey groups. Lipid peroxidation, glutathione, and nitric oxide (NO) levels, as well as glutathione-S-transferase and superoxide dismutase activities were measured in the brain and small intestine. SDS-polyacrylamide gel electrophoresis of the brain and intestine tissues were also carried out.Results: While MTX treatment caused oxidative damage in the brain and small intestine, whey protein administration ameliorated MTX-induced oxidative stress. MTX administration did not change the brain’s NO level, while an increase in intestinal NO level was detected.Conclusion: MTX induced oxidative stress in the brain and small intestine changed the protein metabolism in these tissues regardless of reduced food intake. Consecutive 10-day administration of whey proteins has shown its therapeutic effect on MTX-induced brain and small intestine oxidative damage. KW - Methotrexate KW - whey protein KW - brain KW - small intestine KW - nitric oxide KW - oxidative stress CR - 1. Hannoodee M, Mittal M. Methotrexate. 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