TY  - JOUR
T1  - Gestasyonel Diyabet ile IL8/CXCL8 rs4073 Gen Polimorfizmi Arasındaki İlişkinin Araştırılması
TT  - Investigation of the Relationship Between Gestational Diabetes Mellitus and IL8/CXCL8 rs4073 Gene Polymorphism
AU  - Çakmak Genç, Güneş
AU  - Karakaş Çelik, Sevim
AU  - Hazaloğlu, Mürsel
AU  - Harma, Müge
AU  - Dursun, Ahmet
PY  - 2023
DA  - April
Y2  - 2023
DO  - 10.25048/tudod.1198053
JF  - Turkish Journal of Diabetes and Obesity
JO  - Turk J Diab Obes
PB  - Zonguldak Bulent Ecevit University
WT  - DergiPark
SN  - 2587-0335
SP  - 66
EP  - 71
VL  - 7
IS  - 1
LA  - tr
AB  - Amaç: Gestasyonel Diyabet (GDM) ilk defa gebelikte tanı konulan glukoz intoleransıdır. Hem anne hem de bebekte komplikasyonriskini artıran GDM için ileri gebelik yaşı, çok sayıda parite, önceki gebelikte GDM öyküsü, genetik faktörler gibi birçok risk faktörütanımlanmıştır. Ayrıca bazı inflamatuar mediatörler, kemokinler ve onları kodlayan genlerin polimorfizmlerinin GDM ile ilişkisigösterilmiştir. Biz bu çalışmada GDM tanısı konulan gebelerle sağlıklı gebelerdeki genotip ve allel frekans dağılımlarını karşılaştırarak,GDM yatkınlığı ile IL8/CXCL8 rs4073 (251A/T) gen polimorfizminin ilişkisini belirlemeyi amaçladık.Gereç ve Yöntemler: Çalışmamıza GDM tanılı 100 gebe ve kontrol grubu olarak 100 sağlıklı gebe dahil edilmiştir. Tüm katılımcılarZonguldak Bülent Ecevit Üniversitesi Tıp Fakültesi Hastanesi Kadın Hastalıkları ve Doğum Anabilim Dalı’nda takip edilmiştir. IL8/CXCL8 rs4073 gen polimorfizminin genotiplenmesi, polimeraz zincir reaksiyonu bazlı restriksiyon fragman uzunluk polimorfizmi(PCR-RFLP) yöntemi kullanılarak belirlenmiştir. GDM hastalarında ve kontrollerde her bir gen polimorfizminin genotip sıklığınıkarşılaştırmak için χ2 testi kullanıldı.Bulgular: Gestasyonel diyabetli gebelerle sağlıklı gebeler arasında rs4073 polimorfizmi genotip ve allel frekans dağılımları açısındanistatistiksel olarak anlamlı bir farklılık saptanmamıştır (sırasıyla; p=0,260, p=1,000).Sonuç: Bulgularımız IL8/CXCL8 rs4073 gen polimorfizminin GDM’ye yatkınlıkla ilişkili olmadığını göstermekle birlikte bulgularımızındoğrulanması için farklı etnik grupları içeren geniş örneklem büyüklükleriyle, katılımcıların vücut kütle indeksleri, gebelik öncesikiloları gibi klinik parametreler de dahil edilerek yapılacak ileri çalışmalara ihtiyaç vardır
KW  - Gestasyonel Diyabet
KW  - IL-8
KW  - CXCL8
KW  - rs4073
KW  - gen polimorfizm
N2  - Aim: Gestational Diabetes (GDM) is glucose intolerance that is first diagnosed during pregnancy. Many risk factors such as advancedgestational age, multiparity, history of GDM in a previous pregnancy, and genetic factors have been defined for GDM, which increasesthe risk of complications in both mother and baby. In addition, some inflammatory mediators, chemokines, and polymorphisms of thegenes encoding them are associated with GDM. In this study, we aimed to determine the relationship between GDM susceptibility andIL8/CXCL8 rs4073 (-251A/T) gene polymorphism by comparing the genotype and allele frequency distributions in pregnant womendiagnosed with GDM and healthy pregnant women.Material and Methods: One hundred pregnant patients diagnosed with GDM and 100 healthy pregnant as the control group, wereincluded in our study. All participants were followed up in Zonguldak Bülent Ecevit University Medical Faculty Hospital, Department of Obstetrics and Gynecology. Genotyping of the IL8/CXCL8 rs4073 gene polymorphism was determined using the polymerase chainreaction-based restriction fragment length polymorphism (PCR-RFLP) method. The χ2 test was used to compare the genotype frequencyof each gene polymorphism in GDM patients and controls.Results: There was no statistically significant difference between pregnant women with GDM and healthy pregnant women in terms ofrs4073 polymorphism genotype and allele frequency distributions (p=0.260, p=1.000, respectively).Conclusion: Although our findings show that IL8/CXCL8 rs4073 gene polymorphism is not associated with susceptibility to GDM,further studies with large sample sizes including different ethnic groups and clinical parameters of participants such as body mass indexand pre-pregnancy weight, are needed to confirm our findings
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UR  - https://doi.org/10.25048/tudod.1198053
L1  - https://dergipark.org.tr/en/download/article-file/2745279
ER  -